Evaluation of bleeding on probing and gingival crevicular fluid enzyme activity for detection of periodontally active sites during supportive periodontal therapy

Abstract

This study aimed to analyze the enzyme activity in gingival crevicular fluid (GCF) and its association with clinical parameters, especially bleeding on probing (BOP), and thus reconsider the significance and accuracy of recording BOP. A total of 184 patients who had entered supportive periodontal therapy were selected and GCF was collected from 401 sites before recording the clinical parameters, probing pocket depth (PPD), BOP, clinical attachment level, gingival index and plaque index. The enzyme activity of neutrophil elastase and aspartate aminotransferase and amount of protein in GCF were also analyzed. In the clinical parameters for biochemical data, amount of GCF showed the most correlation. A cut-off value for BOP and PPD were determined by the ROC curve and Youden index. Analysis was performed with all clinical parameters and biochemical data. Of the 401 sites, 51 were less than the cut-off value and were BOP-negative. On the other hand, 29 sites had values more than the cut-off value, with 14 BOP-negative sites and 15 BOP-positive sites. A conclusion is as follows: twenty-nine sites with values more than the cut-off value were diagnosed as sites requiring periodontal management, however, 14 of these were BOP-negative. These results suggest that combining other biochemical tests with examination of BOP and PPD may improve the validity of periodontal disease diagnosis. In future studies, it will be essential to find a marker that can precisely detect periodontal disease activity, and to develop a diagnostic tool for chair-side use.

Association of chairside salivary aMMP-8 findings with periodontal risk assessment parameters in patients receiving supportive periodontal therapy

“…The observation of an association between aMMP-8 levels and gingival inflammation (mSBI) in the present study could support the latter possibility. However, the literature shows that aMMP-8 concentrations increase with the severity of periodontitis and that aMMP-8 levels are directly associated with BOP and PPD [3132333435]. This conflicts with the findings of the current study.…”

Accuracy of single molecular biomarkers in gingival crevicular fluid for the diagnosis of periodontitis: A systematic review and meta‐analysis

“…Those articles with at least one positive word and no negative word were selected as candidates for their full text to be assessed. The analysis of the positive and negative words was carried out using the tm package, version 0.7-5, and NLP package,version 0.1-11 (Feinerer, Hornik, & Meyer, 2008;Hornik, 2017).…”

Analysis of Subgingival Plaque Ability to Stimulate Toll‐Like Receptor 2 and 4

“…4). In particular, larger amounts of gingival crevicular fluid can be found at sites with BOP compared with sites without BOP 22 , 23 . As a result, it could lead to even fewer numbers of total bacteria in samples from sites with BOP (Fig.…”