Evaluation of Bone Tissue Response to a Sealer Containing Mineral Trioxide Aggregate

Assmann, Eloísa; Böttcher, Daiana Elisabeth; Hoppe, Carolina Bender; Grecca, Fabiana Soares; Kopper, Patrícia Maria Poli

doi:10.1016/j.joen.2014.09.019

Cited by 44 publications

(54 citation statements)

References 34 publications

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“…However, it has demonstrated insufficient biocompatibility effects on bone and subcutaneous connective tissue (15,16). In addition, the antibacterial activity of MTAbased sealers remains a limitation, since the material presents an Different letters indicate statistically significant difference between materials within the experimental times (p<0.05).…”

Section: Discussionmentioning

confidence: 99%

Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

et al. 2017

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This study assessed the pH, radiopacity, antimicrobial effect, cytotoxicity and biocompatibility of endodontic filling materials for primary teeth. Zinc oxide eugenol (ZOE), Vitapex and Calen paste thickened with zinc oxide (ZO) were evaluated in comparison to an experimental MTA-based material. Radiopacity was tested using a graduated aluminum stepwedge with a digital sensor (n=5). The materials pH was recorded at 1, 4, 12 h; 1, 3, 7, 15 and 30 days (n=5). Direct contact test was used to assess the antimicrobial efficacy against Enterococcus faecalis after 1, 4, 12, 24 h (n=5). Cytotoxicity assay used MTT test for cell viability after incubation for 1, 3 and 7 days (n=5). For biocompatibility test, Wistar rats had received implants containing each material (n=5). The biopsied tissues were histologically analyzed after 15, 30 and 60 days. The results of radiopacity, pH, antimicrobial capacity and cytotoxicity were analyzed using ANOVA and Tukey tests. The histological data were submitted to Kruskal-Wallis test. The experimental material presented the lowest radiopacity (3.28 mm Al) and had a pH>12.0 throughout the test period. The experimental material showed the highest antibacterial effect, killing over 99.97% bacteria in 4 h. Vitapex presented the highest cell viability. Initially, biocompatibility test showed moderate to severe inflammation in all groups. After 60 days, Calen+ZO group showed moderate inflammation, while the others showed predominantly mild inflammatory reaction. The present results demonstrated that the experimental MTA-based material exhibited satisfactory behavior regarding the studied properties. Additional in vivo studies are necessary for a better evaluation of the material. Physicochemical and Biological Evaluation of Endodontic Filling M a t e r i a l s f o r P r i m a r y Te e t h

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Section: Discussionmentioning

confidence: 99%

Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

et al. 2017

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“…It was created in an effort to combine a material with excellent biocompatibility and bioactive potential such as MTA with another material with very good physical properties such as synthetic resins. However, recent research has provided contradictory results for this sealer regarding cytotoxicity and genotoxicity [17–20]. …”

Section: Introductionmentioning

confidence: 99%

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Victoria-Escandell

Ibáñez-Cabellos

Cutanda

et al. 2017

Stem Cells International

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Human dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative and root canal therapies. This study explores the cytotoxicity and genotoxicity mediated by oxidative stress of three endodontic materials that are widely used on HDPSCs: a mineral trioxide aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an MTA-based cement sealer (MTA-Fillapex). Cell viability and cell death rate were assessed by flow cytometry. Oxidative stress was measured by OxyBlot. Levels of antioxidant enzymes were evaluated by Western blot. Genotoxicity was studied by quantifying the expression levels of DNA damage sensors such as ATM and RAD53 genes and DNA damage repair sensors such as RAD51 and PARP-1. Results indicate that AH-Plus increased apoptosis, oxidative stress, and genotoxicity markers in HDPSCs. MTA-Fillapex was the most cytotoxic oxidative stress inductor and genotoxic material for HDPSCs at longer times in preincubated cell culture medium, and MTA-Angelus was less cytotoxic and genotoxic than AH-Plus and MTA-Fillapex at all times assayed.

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“…Several studies have reported that MTA yielded a significantly higher frequency of mineralized bridge formation, thicker and less porous dentin, and less periapical inflammation than calcium hydroxide 5,6) . It has also been reported that MTA induces migration and proliferation of osteogenic and odontoblastic cells, which, in turn, promotes mineralized tissue deposition, reducing inflammation in its site of action 4,6,8) . Such a protocol, combining the use of these two biomaterials (calcium hydroxide and MTA) in treating teeth with incomplete root formation, has been reported to offer favorable outcomes [1][2][3] , and the present results support the findings of these earlier studies.…”

Section: Discussionmentioning

confidence: 96%

Mineral Trioxide Aggregate for Intruded Teeth with Incomplete Apex Formation

Oliveira

Carvalho

Gonçalves

et al. 2018

Bull. Tokyo Dent. Coll.

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The axial displacement of a tooth within the alveolar bone is called traumatic intrusive luxation. The treatment of immature permanent teeth with incomplete root formation is a challenging procedure, as the prognosis is uncertain. The objective of the present article is to report the successful treatment of traumatic intrusive luxation in teeth with incomplete root formation, where mineral trioxide aggregate (MTA) was used as an apical plug to induce apexification. A 10-year-old boy was referred to our department for emergency treatment of dentoalveolar trauma to the maxillary central incisors. After clinical and radiographic examination, the teeth were surgically repositioned and rigidly fixed. Three months later, a pulp vitality test of both teeth elicited a negative response. Endodontic therapy with an MTA plug was used to induce apexification as root formation was incomplete. The root canals were then filled. Clinical and radiographic examination was then performed again at 2 and 4 months later. The MTA apical plug was effective in inducing apexification and maintaining both teeth.

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Evaluation of Bone Tissue Response to a Sealer Containing Mineral Trioxide Aggregate

Cited by 44 publications

References 34 publications

Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Mineral Trioxide Aggregate for Intruded Teeth with Incomplete Apex Formation

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