Evaluation of buffer solutions and urea addition for estimating the in vitro digestibility of feeds

Camacho, Larissa Frota; Silva, Tadeu E; Palma, Malber Nathan Nobre; Assunção, Amanda S.; Rodrigues, João Paulo Pacheco; Silva, Luiz Fernando Costa e; Detmann, Edênio

doi:10.1093/jas/sky464

Cited by 12 publications

(10 citation statements)

References 22 publications

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“…In each jar, 400 mL of ruminal inoculum and 1600 mL of McDougall’s buffer solution were added. The preparation of buffer solution followed the procedures described by Camacho et al [ 10 ]. Carbon dioxide was flushed into the headspace of each jar, which was closed and placed into the preheated (39 °C) artificial fermenter.…”

Section: Methodsmentioning

confidence: 99%

“…For the in vitro assay, test portions of 500 mg of each feed were weighed and stored in heat-sealed filter bags (non-woven textile 100 g/m 2 ; 4 × 4.5 cm; [ 12 ]). Moreover, all reagents necessary to compose 10 L of McDougall’s buffer solution [ 10 ] were weighed and stored in labelled plastic bags.…”

Section: Methodsmentioning

confidence: 99%

“…The in vitro digestibility can be affected by several alterations in the standard procedures, such as laboratory instruments, vessel and filter bag types, buffer solutions, headspace gas type, way of incubating samples, analyst working, inoculum sources, inoculum donor diet, sample grinding, and others [ 2 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Any change either in the number of steps or in any parameter of the analysis will result in different in vitro methods whose digestibility estimates cannot be directly compared with each other.…”

Section: Introductionmentioning

confidence: 99%

“…In order to do this, the study director’s laboratory at the Brazilian National Institute of Science and Technology in Animal Science (INCT-CA) developed several studies to establish standard methods of in vitro and in situ digestibility for ruminant feeds or diets (e.g., [ 2 , 10 , 12 , 13 ]). An integrated method using rumen fermenters was derived from those studies and has been successfully applied within the study director’s laboratory.…”

Section: Introductionmentioning

confidence: 99%

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A Standard Procedure for In Vitro Digestion Using Rumen Fermenters: A Collaborative Study

Camacho

Silva

Rodrigues

et al. 2022

Animals

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Our objective was to propose and evaluate a standard procedure for the evaluation of in vitro dry matter digestibility for ruminant feeds, using artificial fermenters. A collaborative study was performed with seven feed analysis laboratories and four feeds (Tifton 85 hay, corn silage, soybean hulls, and soybean meal). Two types of artificial fermenters were evaluated (DaisyII Ankom and TE-150 Tecnal). Each laboratory received 80 sealed filter bags with samples (20 per feed), eight blank filter bags, a plastic bag with buffer solution reagents, and instructions describing how to conduct a 48 h in vitro assay using an artificial fermenter and how to collect bovine ruminal inoculum. On average, the contribution of laboratory effect to the total random variance was 24%, being less than the contribution of equipment (42%) and error (34%). The repeatability ranged from 3.34 to 5.79%, across feeds. The reproducibility ranged from 5.93 to 8.94% across feeds, which implied Horwitz ratios ranging from 2.94 to 4.10. Due to the specific characteristics of the analytical entity evaluated here, which is defined by the method itself, the proposed method was considered reproducible. The results highlighted that, if the method is followed exactly, its results are precise and present adequate levels of repeatability and reproducibility.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Standard Procedure for In Vitro Digestion Using Rumen Fermenters: A Collaborative Study

Camacho

Silva

Rodrigues

et al. 2022

Animals

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“…Solution B (g/100 mL) was composed of 15.0 g sodium carbonate (Na 2 CO3) and 1.0 g sodium sulfide (Na 2 S.9H 2 O). The solutions were mixed in the ratio 1: 5 reaching pH 6.8 at the constant temperature of 39 °C ( Silva and Queiroz, 2002 ; Camacho et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Degradability, in vitro fermentation parameters, and kinetic degradation of diets with increasing levels of forage and chitosan

Jacaúna

Goes

Seno

et al. 2021

Translational Animal Science

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Chitosan is the second most important natural biopolymer in the world, extracted from crustaceans, shrimps, and crabs; and can modulate rumen fermentation. Our hypothesis is that the addition of chitosan alters the fermentation patterns of different diets for ruminants. This study aimed to evaluate the effects of different levels of chitosan and forage on in vitro dry degradation kinetics and fermentation in a gas production system. The chitosan levels (0, 1625, 3500 or 7500 mg/kg of DM) were arranged in a completely randomized block design, and for in vitro ruminal fermentation assay we used a split splot arrangement. Into the incubator, all chitosan levels were distributed in the four jars, and the forage levels varying on 100, 65, 50, 35 and 20 on DM basis. Chitosan and roughage levels interaction effect (P≤0.05) on IVDMD; IVOMD. IVDCP and IVDNDF. Chitosan negatively affected IVDMD in all roughage levels evaluated. The pH and ammonia concentration present effect only for roughage levels and incubation hours. The chitosan didn’t change (P=0.3631) the total short-chain fatty acid concentration (overall mean = 21.19 mmol/L) and the C2:C3 ratio (overall mean = 5.85). The IVDCP showed the same decreasing quadratic behavior (P<0.0001). The increasing chitosan addition increases (P<0.0001) the gas production and decreases the (P<0.0001) the lag time (parameter C) of diets with greater concentrate participation, characterizing greater efficiency in the degradability of the diet, confirming its potential use in diets for ruminants. Chitosan changes in vitro dry degradation kinetics and fermentation at the minimum dose of 1722 mg/kg DM for all diets. The roughage level influenced the in vitro nutrients degradability and cumulative gas production.

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In vitro Effects of Cellulose Acetate on Fermentation Profiles, the Microbiome, and Gamma-aminobutyric Acid Production in Human Stool Cultures

et al. 2023

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Evaluation of buffer solutions and urea addition for estimating the in vitro digestibility of feeds

Cited by 12 publications

References 22 publications

A Standard Procedure for In Vitro Digestion Using Rumen Fermenters: A Collaborative Study

A Standard Procedure for In Vitro Digestion Using Rumen Fermenters: A Collaborative Study

Degradability, in vitro fermentation parameters, and kinetic degradation of diets with increasing levels of forage and chitosan

In vitro Effects of Cellulose Acetate on Fermentation Profiles, the Microbiome, and Gamma-aminobutyric Acid Production in Human Stool Cultures

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