Evaluation of Cell Behaviour on Atmospheric Plasma Deposited Siloxane and Fluorosiloxane Coatings

Ardhaoui, Malika; Naciri, Mariam; Mullen, Tracy; Brugha, Cathal M.; Keenan, A. K.; Al‐Rubeai, Mohamed; Dowling, Denis P.

doi:10.1163/016942409x12598231567943

Cited by 14 publications

(9 citation statements)

References 43 publications

(24 reference statements)

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“…Both of which have not been reported before. These measurements allow the reported plasma processing outcome [12][13][14][15][16][17][18][19] to be revaluated in terms of discharge power and heat transfer. Outside the nozzle the gas temperature drops towards the surface to such a degree that allows heat sensitive treatment of polymeric and composite materials and many medical applications.…”

Section: Discussionmentioning

confidence: 99%

“…Outside the nozzle the gas temperature drops towards the surface to such a degree that allows heat sensitive treatment of polymeric and composite materials and many medical applications. By selecting an appropriate gap distance of 2-3 mm and 50% of the power supply setting (*5 W discharge power), Ardhaoui et al [16] has shown that the effective temperature and abundance of activated species can be actively engineered for cell adhesion. This conclusion also has relevance to the adhesive bonding issue highlighted in the introduction where high adhesive bond strength for siloxane elastomers bonded to coated steel were obtained at the more energetic smaller gap distances between the nozzle and the substrate.…”

Section: Discussionmentioning

confidence: 99%

“…The main difference between this system and the plasma needle, pencil and microplasma jet systems is the electrode structure, the increased electrode to treated surface distance and the increased nozzle area. Amongst the applications reported to-date of this source are: enhancing silicone adhesion to steel [14,15], control of cell adhesion [16] and the deposition of plasma polymerized coatings [17,18]. From these studies it has been concluded that parameters such as applied power and the gap distance between the substrate and the jet nozzle significantly influence deposited coating properties.…”

Section: Introductionmentioning

confidence: 99%

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Electrical, Thermal and Optical Diagnostics of an Atmospheric Plasma Jet System

Nwankire

Law

Nindrayog³

et al. 2010

Plasma Chem Plasma Process

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Plasma diagnostics of atmospheric plasmas is a key tool in helping to understand processing performance issues. This paper presents an electrical, optical and thermographic imaging study of the PlasmaStream atmospheric plasma jet system. The system was found to exhibit three operating modes; one constricted/localized plasma and two extended volume plasmas. At low power and helium flows the plasma is localized at the electrodes and has the electrical properties of a corona/filamentary discharge with electrical chaotic temporal structure. With increasing discharge power and helium flow the plasma expands into the volume of the tube, becoming regular and homogeneous in appearance. Emission spectra show evidence of atomic oxygen, nitric oxide and the hydroxyl radical production. Plasma activated gas temperature deduced from the rotational temperature of nitrogen molecules was found to be of order of 400 K: whereas thermographic imaging of the quartz tube yielded surface temperatures between 319 and 347 K.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrical, Thermal and Optical Diagnostics of an Atmospheric Plasma Jet System

Nwankire

Law

Nindrayog³

et al. 2010

Plasma Chem Plasma Process

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“…These measurements were taken one week after the coatings were deposited in order to minimise activation effects of the plasma on the silicon wafer substrate. [28] Surface energies were determined using deionised water, diodomethane and ethylene glycol. The surface energy of the deposited coatings was then calculated using the OWRK method.…”

Section: Surface Characterisationmentioning

confidence: 99%

“…This technique in which a liquid precursor is nebulized into a plasma jet has previously been used for the deposition of a range of plasma polymerised coatings such as siloxanes and fluoropolymers. [28,29] In this study the QAS precursor investigated was dimethyloctadecyl [3-trimethoxysilyl) propyl] ammoniumchloride (C 26 H 58 ClNO 3 Si), also known as ODAMO. This precursor was selected for the protein adhesion studies due to its low toxicity, which is an important consideration in the nebulisation of this precursor into a plasma at atmospheric pressure.…”

Section: Introductionmentioning

confidence: 99%

Protein Adhesion on Atmospheric Plasma Deposited Quaternary Ammonium Salt Coatings

Donegan

Dowling

2013

Plasma Processes & Polymers

Self Cite

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This study investigates protein adhesion on nm thick helium atmospheric plasma deposited quaternary ammonium salt (QAS) coatings. The adhesion of the proteins BSA, IgG and Fg was evaluated on coated and uncoated silicon wafer substrates. This study was carried out in PBS solution, under flow conditions using ellipsometry. The QAS was found to exhibit a low level of solubility in PBS over time (approx. 2 nm / hour). On addition of both the IgG and Fg proteins, it was found that a protective protein layer of 7 and 2 nm respectively was formed, which prevented further dissolution of the QAS. In contrast the 1 nm thick BSA protein layer, which formed on the QAS, was insufficiently thick to prevent the slow dissolution of the salt. It was concluded that the charge and structure of the protein influences its adhesion on the QAS surface. IntroductionA key issue affecting the performance of medical devices in the body is protein adhesion. [1,2] This is because proteins dictate how the cell interacts with the device surface.[3] Although there is a degree of inconsistency within the literature as to the factors influencing protein adsorption on a surface, it is believed that chemical functionality and topography are important factors. [4] Amongst the techniques that have been investigated to alter the surfaces of polymers in order to modify protein adhesion have been micro patterning and monomer polymerisation by free radical solution, emulsion, grafting as well as plasma processes. [5][6][7][8][9] The focus of this study is to investigate how a plasma polymerised quaternary ammonium salt (QAS) coating deposited onto a silicon wafer substrate influences protein adhesion.Worldwide consumption of quaternary ammonium compounds is estimated to be approximately 700,000 tons per annum.[10] Applications of these salts vary from surfactants, corrosion inhibitors and pesticides to personal care products and fabric softeners. [10][11][12] QAS coatings were selected for this study as they have also been reported to exhibit anti-bacterial and anti-fungal properties. [13,14] QAS surfaces have been shown to exhibit a high positive charge density, which exerts a strong electrostatic interaction with negatively charged bacteria. [15] It is reported that after the microbial cell adsorbs onto the coating surface through electrostatic interactions, the alkyl chain of the QAS, if of sufficient length, penetrates the microbial cell wall. This can disrupt the cytoplasmic membrane and release toxins that lead to necrosis. It is suggested that positively charged surfaces can tightly bind the adsorbed microbial cells and prevent their subsequent growth and proliferation, including biofilm formation.[16]There have been relatively few reports on the interaction between QAS surfaces and proteins.There have also been some inconsistencies between the adhesion results obtained by different authors. [17][18][19] It has been shown that quaternary ammonium based polymer coatings provide effective resistance against the non-specific adsorption of proteins ...

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Evaluation of the physico‐mechanical properties, mineralization capability, degradability and biological behavior of PCL/SBG composite scaffolds fabricated by three‐dimensional fiber deposition

Li,

Liu,

Jiang

et al. 2024

Polymer Composites

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In bone tissue engineering, polycaprolactone (PCL) has been extensively employed as a bone substitute due to its good biocompatibility, outstanding mechanical properties, controllable degradation rate, and excellent processability. However, PCL is a bioinert polymer with poor hydrophilicity, and it fails to actively induce new bone formation after implantation into the lesion. Therefore, it is generally necessary to find a bioactive material to improve these deficiencies of PCL. In this study, we first prepared a silicate‐based bioactive glass (SBG, 48SiO2‐24Na2O‐24CaO‐4P2O5, in mol%) using the melt‐quenching method, and then fabricated a series of PCL/SBG composite scaffolds with varying SBG powder contents (0, 10, 20, 30, and 40 wt.%) by 3D fiber deposition. The physico‐mechanical properties, mineralization capability, degradability and biological behavior of PCL/SBG scaffolds were systematically characterized. The results showed that the contact angle of the scaffolds decreased from 121.90 ± 3.74° to 86.29 ± 3.46° with the increase of SBG content, implying that the hydrophilicity was improved, and meanwhile the porosity and mechanical strength of PCL/SBG scaffolds first increased and then decreased, but they were within the range of human cancellous bone. Additionally, the degradation rate of PCL/SBG groups can be regulated by adjusting the SBG content. Finally, compared to the pure PCL group, the cell viability and alkaline phosphatase activity of MC3T3‐E1 on PCL/SBG groups were significantly enhanced. In the present work, the PCL/30SBG group exhibited great potential as a viable alternative to autografts and offers promising clinical applications for bone defect repair in the future.Highlights The addition of SBG powders accelerates the degradation of PCL. The addition of SBG powders enhances the hydrophilicity of PCL scaffolds. The degradation rate and bioactivity of PCL/SBG scaffolds can be regulated. The mechanical strength is within the range of human cancellous bone. PCL/30SBG shows improved cell viability and ALP activity.

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Evaluation of Cell Behaviour on Atmospheric Plasma Deposited Siloxane and Fluorosiloxane Coatings

Abstract: Osteoblasts >Hepatocytes> CHO. The HEK and THP-1 inflammatory cells in contrast were not found to be sensitive to changes in water contact angle.

Cited by 14 publications

References 43 publications

Electrical, Thermal and Optical Diagnostics of an Atmospheric Plasma Jet System

Electrical, Thermal and Optical Diagnostics of an Atmospheric Plasma Jet System

Protein Adhesion on Atmospheric Plasma Deposited Quaternary Ammonium Salt Coatings

Evaluation of the physico‐mechanical properties, mineralization capability, degradability and biological behavior of PCL/SBG composite scaffolds fabricated by three‐dimensional fiber deposition

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