This chapter explores the ethical implications of chatbots and AI assistants, focusing on privacy and data security, bias and fairness, transparency and accountability, user autonomy, employment impacts, manipulation and persuasion, user well-being, cultural sensitivity, and sustainability. Privacy and data security are paramount, emphasizing user consent and trust in data handling. Bias in AI systems can perpetuate discrimination, necessitating proactive measures for fairness. Transparency and accountability are critical for user trust, requiring clear communication and regulatory frameworks. User autonomy must be respected, allowing control over AI interactions. The impact on employment highlights the need for strategies to manage job displacement and skill development. AI's potential for manipulation necessitates ethical guidelines to prevent misuse. Ensuring user well-being involves addressing mental health risks and misinformation. Cultural sensitivity in AI design promotes inclusivity. Sustainability considerations address the environmental impact of AI systems.