Evaluation of chemical constituents from Glehnia littoralis for antiproliferative activity against HT-29 human colon cancer cells

Medicinal halophytes: potent source of health promoting biomolecules with medical, nutraceutical and food applications

“…An invention related to the utilization of Cakile maritima extract in cosmetic composition, especially a moisturizing composition for skin was due to the presence of an active principle belonging to the glucosinolate group (Fabre and Dussert, 2000). Um et al (2010) isolated and identified four furanocoumarines (Bergapten, isopimpinellin, xanthotoxin, and imperatoxin) from 85% aqueous MeOH solvent fraction in Glehnia littoralis (Apiaceae). This fraction exhibited a high antiproliferative effect, induced apoptosis as determined by 4,6-diamidino-2-phenylindole (DAPI) staining, and reduced mRNA expression of Bcl-2, cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS).…”

“…The order of their antiproliferative effect varied according to the kinds of used cancer cells (Fujioka, 1999). For HT-29 human colon cancer cells, isopimpinellin and xanthotoxin isolated from Glehnia littoralis exhibited higher antiproliferative activity than two other furanocoumarine compounds (Um et al, 2010).…”

“…Considering the potential health benefits of falcarindiol, the results of these investigations suggest that C. maritimum could be used in food manufactures and cosmetology as preservative agents, and biopesticides, or in medicine as new antibiotics, confirming the interest in studying halophytic species as sources of bioactive compounds. Um et al (2010) isolated and identified 3 polyacetylenes (panaxidiol, falcarindiol, falcarinol) from n-hexane solvent fraction in Glehnia littoralis. This fraction exhibited a high antiproliferative effect, induced apoptosis as determined by 4,6-diamidino-2-phenylindole (DAPI) staining, and reduced mRNA expression of Bcl-2, cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS).…”

Analytical Methods for Isolation, Separation and Identification of Selected Furanocoumarins in Plant Material

“…The furanocoumarin determination from air-dried plant material was also performed using 75% methanol in an ultrasonic ice-water bath , with 100% methanol (Cardoso et al, 2000;Ojala, 2000), 70% methanol (Chen & Sheu, 1995), with hot (70˚C) pure methanol on a water bath (Bartnik & Głowniak, 2007), with pure ethanol in heated reflux (Yu et al, 2002), with 95% ethanol at 80˚C Zheng et al, 2010), with acetone at room temperature (Taniguchi et al, 2011), with ether at 40˚C (Liu et al, 2004a), with dichloromethane at room temperature (Um et al, 2010), with petroleum ether at room temperature (Tosun et al, 2008), with chloroform in a sonic bath (Cardoso et al, 2000).…”

“…The reader is referred to Rojas-Lima et al, 1999;Um et al, 2010;Taniguchi et al, 2011, andTesso et al, 2005 publications, for details of the principles of NMR and general interpretation of NMR spectra.…”