The field experiment entitled “Assessment of bioefficacy by using biostimulants on growth, quality and yield of chilli (capsicum annum l.)”. was conducted during rabi season August 2022 to January 2023 at Horticulture Research Farm, Department of Horticulture, Naini Agricultural Institute, SHUATS, Prayagraj, (U. P). The experiment had sixteen treatments replicated thrice in a randomized block design. The results from the present investigation concluded that bio stimulant which is applied in T 8 Dollar 2.5 ml / L was found superior in all aspects viz, Average Plant height (62.80), Average number of Primary branches (4.33), Average number of secondary branches (8.36), Plant surface area (56.62 cm2), No of days of flowering (52.81), Average number of flowers/cluster (12.42), Average number of flower/ plant (61.96), no of fruits/plants (73.66), Fruit length (12.26 cm), fruit girth (2.85 cm), Average Yield (612.81 gm), yield/kg (3.68 kg), Yield tonn/ha (22.68), Total soluble solids (0Brix) (5.22), Ascorbic acid (118.52 mg/100 g).Among the treatment maximum net profit in T8 (Dollar 2.5 ml / L) @ Rs/ha is 314765.85 & B:C ratio is 3.26 were recorded significantly higher compared to other treatments.