Evaluation of chiral separation based on bovine serum albumin–conjugated carbon nanotubes as stationary phase in capillary electrochromatography

Abstract

In this work, we utilized adsorbed BSA and multiwalled carbon nanoparticles (BSA/MWCNTs) as a stationary phase in open tubular (OT) capillary for separation of chiral drugs. (3-Aminopropyl)triethoxysilane was used to assist fabrication of BSA/MWCNTscoated OT column by covalent bonding. Incorporation of MWCNTs nanomaterials into a polymer matrix could increase the phase ratio and take advantage of the easy preparation of an open tubular CEC column. SEM was carried out to characterize the BSA/MWCNTs OT columns. The electrochromatographic performance of the OT columns was evaluated by separation of ketoprofen, ibuprofen, uniconazole, and hesperidin. The effects of MWCNTs concentration, background solution pH and concentration, and applied voltage on separation were investigated. Chiral separations of ketoprofen, ibuprofen, uniconazole, and hesperidin were achieved using the BSA/MWCNTs-coated OT column with resolutions of 24.20, 12.81, 1.50, and 1.85, respectively. Their optimas were found in the 30 mM phosphate buffers at pH 5.0, 6.5, 7.0, and 6.5, respectively. In addition, the columns demonstrated good repeatability and stability with the run-to-run, day-today , and batch-to-batch RSDs of migration times less than 3.5%.

Recent applications and chiral separation development based on stationary phases in open tubular capillary electrochromatography (2019–2022)

“…Overall, we provide readers interested in OT-CEC with the most up-to-date overview of this field, and the related new SPs from 2019 to 2022 are listed in Table 1 [ 3 , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] ]. PubMed and Web of Science were used to search the following characters (also their acronyms) alone or in combination: “Open tubular”, “Capillary electrochromatography”, “Chiral separation”, “Stationary phase”, and “Coating” for relevant literature to analyze recent OT-CEC developments.…”

β‐Cyclodextrin covalent organic framework supported by polydopamine as stationary phases for electrochromatographic enantioseparation

“…However, due to the limited amount of stationary phase coating, the OT-CEC also suffers from some problems of relatively poor phase ratio and sample capacity, which results in the low separation capability of OT-CEC. To solve these problems and improve the separation effectiveness of the OT-CEC, some materials have been developed to increase the surface area of OT columns, such as metal-organic frameworks [10,11], carbon nanomaterial [12][13][14], gold nanoparticles [15,16] and covalent organic frameworks (COFs) [17][18][19][20].…”

Preparation of a polydopamine/β‐cyclodextrin coated open tubular capillary electrochromatography column and application for enantioseparation of five proton pump inhibitors

“…Chirality is one of the fundamental properties of nature, which can be seen in various fields such as medicine, pesticides, environment, and food [1–4]. Most of the existing drugs have chiral enantiomers to date.…”