Evaluation of Commercially Available Serologic Diagnostic Tests for Chikungunya Virus

Prat, Christine; Flusin, Olivier; Panella, Amanda; Tenebray, Bernard; Lanciotti, Robert S.; Leparc-Goffart, Isabelle

doi:10.3201/eid2012.141269

Cited by 110 publications

(99 citation statements)

References 14 publications

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“…Our results indicated that antibody cross-reactivity with reference mouse and rabbit antisera correlated with antigens derived from closely related species within the same antigen serocomplex. Because previous reports suggested that human MAYV serum samples cross-react with CHIKV and RRV antigens in ELISAs (38)(39)(40), another goal of the study presented here was to provide a more detailed analysis of antigen cross-reactivity. In contrast to the results obtained with animal reference antisera, we observed a high degree of specificity for the three human infection groups examined that was primarily based on independently printed E1 and E2 antigens.…”

Section: Discussionmentioning

confidence: 99%

Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages

Smith

Pugh

Cisney

et al. 2018

mSphere

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Mayaro virus (MAYV), Venezuelan equine encephalitis virus (VEEV), and chikungunya virus (CHIKV) are vector-borne alphaviruses that cocirculate in South America. Human infections by these viruses are frequently underdiagnosed or misdiagnosed, especially in areas with high dengue virus endemicity. Disease may progress to debilitating arthralgia (MAYV, CHIKV), encephalitis (VEEV), and death. Few standardized serological assays exist for specific human alphavirus infection detection, and antigen cross-reactivity can be problematic. Therefore, serological platforms that aid in the specific detection of multiple alphavirus infections will greatly expand disease surveillance for these emerging infections. In this study, serum samples from South American patients with PCR-and/or isolation-confirmed infections caused by MAYV, VEEV, and CHIKV were examined by using a protein microarray assembled with recombinant capsid, envelope protein 1 (E1), and E2 from nine New and Old World alphaviruses. Notably, specific antibody recognition of E1 was observed only with MAYV infections, whereas E2 was specifically targeted by antibodies from all of the alphavirus infections investigated, with evidence of cross-reactivity to E2 of o'nyong-nyong virus only in CHIKV-infected patient serum samples. Our findings suggest that alphavirus structural protein microarrays can distinguish infections caused by MAYV, VEEV, and CHIKV and that this multiplexed serological platform could be useful for high-throughput disease surveillance.IMPORTANCE Mayaro, chikungunya, and Venezuelan equine encephalitis viruses are closely related alphaviruses that are spread by mosquitos, causing diseases that produce similar influenza-like symptoms or more severe illnesses. Moreover, alphavirus infection symptoms can be similar to those of dengue or Zika disease, leading to underreporting of cases and potential misdiagnoses. New methods that can be used to detect antibody responses to multiple alphaviruses within the same assay would greatly aid disease surveillance efforts. However, possible antibody cross-reactivity between viruses can reduce the quality of laboratory results. Our results demonstrate that antibody responses to multiple alphaviruses can be specifically quantified within the same assay by using selected recombinant protein antigens and further show that Mayaro virus infections result in unique responses to viral envelope proteins.

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Section: Discussionmentioning

confidence: 99%

Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages

Smith

Pugh

Cisney

et al. 2018

mSphere

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“…IgM antibody levels peak around 3 to 5 weeks and may persist for 3 months (Prat et al, 2014;Labadie et al, 2010). These tests show variable sensitivity and without a more advanced test kit like ELISA being easily available, standardizing the IgM kits in the field becomes difficult.…”

Section: Resultsmentioning

confidence: 99%

“…In acute phase of the febrile illness, most patients may present with similar clinical symptoms and to alleviate, the clinician has to depend on rapid tests. The rapid POC tests are relatively cheaper, widely available, easy to perform, produce faster results but are subjective and can only be used as screening tests due to low specificity (Stacey et al, 2015;Prat et al, 2014). These rapid tests are critical in differentiating Chikungunya from other arboviral infections to institute the correct emergency procedures.…”

Section: Resultsmentioning

confidence: 99%

Diagnosis of Chikungunya by a Rapid Immunochromatographic Method in a Tertiary Care Setting

Rajgopal¹

2017

Int.J.Curr.Microbiol.App.Sci

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“…The Euroimmun ELISA, which was used this study, had a specificity of 95% (IgG) and a sensitivity of 88% (IgG). The Euroimmun ELISA test was evaluated as to whether it might detect Mayaro and O'Nyong-Nyong virus and positivity was found (20). Until day 7 after disease onset genome detection by RTPCR is recommended for diagnosis.…”

Section: Discussionmentioning

confidence: 99%

A study of the chikungunya virus in humans in Turkey

Atalay¹,

Kaygusuz²,

Azkur³

2017

Turk J Med Sci

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IntroductionThe chikungunya virus (CHIKV) is a single-stranded, positive sense RNA virus that is a member of the genus Alphavirus of the family Togaviridae. The CHIKV is transmitted primarily by Aedes aegypti and Aedes albopictus mosquitoes (1). CHIKV infection led to more than 6 million confirmed cases worldwide (2). CHIKV has a wide geographic distribution including North and South America, Europe, Asia, the Pacific Islands, and Africa since the virus was first described in Tanzania in 1952 (3,4). A female patient who came from India to Turkey with symptoms of fever, arthralgia, and rashes was the first case of laboratory-confirmed CHIKV infection in Turkey (5). Clinical symptoms of CHIKV infection include high fever, fatigue, backache, headache, and polyarthralgia. Polyarthralgia is the most characteristic and common symptom of infection and also the origin of the name of the disease "chikungunya, " a local term in the Makonde language that means "disease that bends up the joints". In addition to these clinical manifestations, tenosynovitis, swollen joints, macular or maculopapular skin rashes, myalgia, nausea, vomiting, and diarrhea can be observed in CHIKV-infected patients (3,6). Differential diagnosis of CHIKV infection from dengue virus infection could be based on clinical manifestations and laboratory features. Fever (over 39 °C), arthritis, arthralgia, rash, and lymphopenia are more significant in a CHIKV infection. While hemoconcentration does not occur in CHIKV infection, it is present in 70%-100% of dengue virus-infected patients (2). Diagnosis of CHIKV infection is based on molecular detection of a viral genome and/or serological detection of virus-specific antibodies. RT-PCR and real-time RT-PCR (7-9) can be used for molecular detection while serological diagnostic tests include ELISA, immunofluorescence assay, and rapid immunochromatographic test (10-13). The aim of this study was to screen for possible exposure to CHIKV infections in humans using ELISA and IIFT in the city of Kırıkkale, which is located in the central Anatolia region of Turkey. Materials and methods SamplesBlood samples were taken from 500 healthy, randomly selected volunteer blood donors who live in Kırıkkale (39°50ʹN; 33°31ʹE; altitude 700 m) through July-November Background/aim: The chikungunya virus (CHIKV) is a mosquito-borne disease and has recently been causing explosive outbreaks. The CHIKV has spread throughout all continents. Although the first chikungunya case imported from India to Turkey was reported in 2012, there is no detailed epidemiologic study in Turkey yet. The aim of this study was to investigate the seroprevalence of the CHIKV in Turkey.Materials and methods: ELISA was used to screen 500 random serum samples of healthy people collected from Kırıkkale, which is located in central Anatolia in Turkey. The results were verified by indirect immunofluorescence test (IIFT). Results:The results showed that 0.4% samples were positive for CHIKV. In the verification study with IIFT, CHIKV IgG type antibodies were defi...

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Evaluation of Commercially Available Serologic Diagnostic Tests for Chikungunya Virus

Cited by 110 publications

References 14 publications

Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages

Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages

Diagnosis of Chikungunya by a Rapid Immunochromatographic Method in a Tertiary Care Setting

A study of the chikungunya virus in humans in Turkey

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