Evaluation of Decellularization in Umbilical Cord Artery

Mallis, Panagiotis; Gontika, Ioanna; Poulogiannopoulos, T.; Zoidakis, Jérôme; Vlahou, Antonia; Michalopoulos, Efstathios; Chatzistamatiou, Theofanis; Papassavas, Andreas; Stavropoulos-Giokas, Catherine

doi:10.1016/j.transproceed.2014.10.027

Cited by 33 publications

(37 citation statements)

References 8 publications

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“…The decellularized HUC artery minimized immunogenicity, supported cell seeding and could be stored for a long term (Gui et al 2009). In addition, decellularized HUC artery lacking of cellular and nuclear residues thus make it suitable for developing nerve graft (Mallis et al 2014). Based on the results of H&E staining and nuclei counterstaining with DAPI in this study, it is concluded that by decellularization process, all residual blood, cells and other soluble components have been successfully removed.…”

Section: Hematoxylin and Eosin (Hande) Staining And Nuclei Counter Stainimentioning

confidence: 68%

“…Sun et al (2011) reported that the use of decellularized artery conduit seeded with differentiated adipose derive stem cell resulted in better nerve regeneration and functional recovery. Apparently, human umbilical cord (HUC) artery might represent a good source as a nerve conduit as it is easily obtainable, simply isolated and lack branches (Mallis et al 2014). The HUC collected from health centres were redundant and often disposed as biological samples.…”

Section: Introductionmentioning

confidence: 99%

“…The use of allograft might provoke immunological response and the recipients should be under immunosuppressive drugs for a certain time. This issue can be avoidable by decellularization process, where this process removes native cells, residual blood and other soluble components leaving the extracellular matrix (ECM) that remained intact (Mallis et al 2014;Quint et al 2011;Tuan-Mu et al 2014). Besides, decellularized blood vessels maintained their three dimensional structure and reduce immunogenicity (Rodríguez-Rodríguez et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Development of Nerve Conduit using Decellularized Human Umbilical Cord Artery Seeded with Centella asiatica Induced-Neurodiferrentiated Human Mesenchymal Stem Cell

Hussin

Idrus²,

Lokanathan³

2018

JSM

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Various natural biological conduits have been investigated to bridge peripheral nerve injury especially in critical gap (greater than 3 cm in human). Autograft, the current gold standard, has several drawbacks including limited availability of donor graft, donor-site morbidity and mismatch in size in clinical practices. The aim of this study was to analyze the development of nerve conduit using decellularized human umbilical cord (HUC) artery seeded with neurodifferentiated human MSCs (ndMSCs) in bridging peripheral nerve gap. Artery conduits obtained from HUC were decellularized to remove native cells (n=3), then characterized by Hematoxylin and Eosin (H&E) staining and nuclei counterstaining with DAPI. The decellularized artery conduit was measured for every 2 weeks until 12 weeks. Next, mesenchymal stem cells (MSCs) were differentiated into neural lineage using 400 µg/mL of Centella asiatica. Then, 1.5×10 6 of MSCs or ndMSCs were seeded into decellularized artery conduit to study cell attachment. H&E staining and nuclei counterstaining with DAPI showed that all cellular components were removed from the HUC arteries. The decellularized artery conduit did not collapse and the lumen remained rigid for 12 weeks. Immunocytochemistry analysis with neural markers namely S100β, P75 NGFR, MBP and GFAP showed that MSCs had differentiated into neural lineage cells. H&E staining showed that the seeded MSCs and ndMSCs attached to the lumen of the conduits as early as 2 days. In conclusion, this study showed that nerve conduit using decellularized HUC artery seeded with neurodifferentiated human MSCs was successfully developed and have the potential to bridge critical nerve gap.

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Section: Hematoxylin and Eosin (Hande) Staining And Nuclei Counter Stainimentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of Nerve Conduit using Decellularized Human Umbilical Cord Artery Seeded with Centella asiatica Induced-Neurodiferrentiated Human Mesenchymal Stem Cell

Hussin

Idrus²,

Lokanathan³

2018

JSM

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“…The aim of this study was to evaluate a previously described decellularization protocol, which was first used on tracheal tissue (8,13). The ultimate goal of this study was to decellularize rat trachea effectively, preserving the ultrastructure and minimizing the number of decellularization cycles required.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Decellularization Protocol for the Development of a Decellularized Tracheal Scaffold

et al. 2018

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“…With their high proliferative potential, karyotype and phenotype stability, differentiation plasticity, paracrine activity, and immunomodulatory properties, UC-derived MSCs can claim the title of the new “gold standard”, ousting the renowned bone marrow-derived MSCs [ 5 – 7 ]. Other examples of UC-derived biomedical products are decellularized UC vessels used as grafts for vascular surgery [ 8 – 10 ] and Wharton’s jelly-derived extracellular matrix for wound healing [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Umbilical cord tissue cryopreservation: a short review

2018

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In this review we present current evidence on the possibility of umbilical cord tissue cryopreservation for subsequent clinical use. Protocols for obtaining umbilical cord-derived vessels, Wharton’s jelly-based grafts, multipotent stromal cells, and other biomedical products from cryopreserved umbilical cords are highlighted, and their prospective clinical applications are discussed. Examination of recent literature indicates we should expect high demand for cryopreservation of umbilical cord tissues in the near future.

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Evaluation of Decellularization in Umbilical Cord Artery

Cited by 33 publications

References 8 publications

Development of Nerve Conduit using Decellularized Human Umbilical Cord Artery Seeded with Centella asiatica Induced-Neurodiferrentiated Human Mesenchymal Stem Cell

Development of Nerve Conduit using Decellularized Human Umbilical Cord Artery Seeded with Centella asiatica Induced-Neurodiferrentiated Human Mesenchymal Stem Cell

Evaluation of a Decellularization Protocol for the Development of a Decellularized Tracheal Scaffold

Umbilical cord tissue cryopreservation: a short review

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