Evaluation of different adjuvant formulations of trivalent Foot and Mouth Disease vaccine in cattle

Abstract

Background Foot-and-mouth disease is globally one of the most economically important viral diseases of cloven-hoofed animals that can be controlled by different strategies, where vaccination plays an important role. Selection of the adjuvants,that added to the vaccine is crucial in ensuring the protective effect of the vaccine. Aluminum hydroxide gel and saponin (AS) is widely used adjuvant, with its poor immune response in FMD vaccine. The present study was undertaken to evaluate different ingredients of adjuvants for inactivated trivalent (A, O and SAT 2) FMD vaccine and to demonstrate the effect of booster dose in cattle. Results Cattle were grouped into five; four experimental and one control, with six animals in each group and immunized with trivalent vaccine with various formulations of adjuvants. Immune response was measured using Solid Phase Competitive Enzyme Linked Immune Sorbent Assay (SPCE). The antibody level in cattle immunised with a vaccine formulation containing a mixture of aluminum hydroxide gel and saponin (AS) were significantly lower than AS boosted group for the three serotypes (p < 0.05, t. test), which directs the need for booster dose. Whereas the antibody response in the AS + oil group was higher followed by oil alone, AS boosted and AS at 95% CI. Conclusion The findings of this study could suggest that oil based and AS with oil could replace the conventional aluminum hydroxide gel and saponin adjuvants in FMD vaccine preparations. On different note, challenge test was not successful in this study indicating the need for further research on the virus infectivity.