Evaluation of Different Advanced Approaches to Simulation of Dynamic In Vitro Digestion of Polyphenols from Different Food Matrices—A Systematic Review

Abstract

Phenolic compounds have become interesting bioactive antioxidant compounds with implications for obesity, cancer and inflammatory gastrointestinal pathologies. As the influence of digestion and gut microbiota on antioxidant behavior is yet to be completely elucidated, and due to limitations associated to in vivo studies, dynamic in vitro gastrointestinal models have been promoted. A systematic review was conducted of different databases (PubMed, Web of Science and Scopus) following PRISMA guidelines to assess different dynamic digestion models and assay protocols used for phenolic compound research regarding bioaccesibility and interaction with colonic microbiota. Of 284 records identified, those including dynamic multicompartmental digestion models for the study of phenolic compound bioaccesibility, bioactivity and the effects of microbiota were included, with 57 studies meeting the inclusion criteria. Different conditions and experimental configurations as well as administered doses, sample treatments and microbiological assays of dynamic digestion studies on polyphenols were recorded and compared to establish their relevance for the dynamic in vitro digestion of phenolic compounds. While similarities were observed in certain experimental areas, a high variability was found in others, such as administered doses. A description of considerations on the study of the digestion of phenolic compounds is proposed to enhance comparability in research.

Wild and Micropropagated Artemisia eriantha Infusions: In Vitro Digestion Effects on Phenolic Pattern and Antioxidant Activity

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Modulation of Swine Gut Microbiota by Phytogenic Blends and High Concentrations of Casein in a Validated Swine Large Intestinal In Vitro Model

“…In this study, we assessed the effects of two phytogenic blends (PB1 and PB2), and their combination with casein on swine gut microbiota in an artificial, dynamic, computer-controlled in vitro large intestine system. Artificial GIT systems are intended to mimic the intestinal physiology of humans and animals, which makes them suitable models for pre-clinical and in vitro studies of the digestibility of foods, feed additives, and drugs and their effects on gut microbiota [ 25 , 26 , 27 ]. Validated artificial GIT models allow for conducting controlled, reproducible, and cost-effective studies, which sometimes are not achievable with in vivo models due to unpredictable factors, such as infections, uncontrolled animal welfare violations, and death of laboratory animals.…”

The role of food structure in gastric-emptying rate, absorption and metabolism

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