Evaluation of different fungi and bacteria strains for production of cellulases by submerged fermentation using sugarcane bagasse as carbon source: Effect of substrate concentration and cultivation temperature

Pinotti, L.; Paulino, Larissa Bastos; Agnezi, Jacyele C.; Santos, PatrÃ­cia A. dos; Silva, Henrique N. L. da; Zavarise, Julio Pansiere; SalomÃ£o, Gabriella S. B.; Tardioli, Paulo Waldir

doi:10.5897/ajb2020.17210

Cited by 8 publications

(2 citation statements)

References 34 publications

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“…An attempt to solve this has led to increase in demand for enzymes to catalyze several industrial processes (Jadhav et al, 2013;Saratale et al, 2014;Mostafa et al, 2019). Microbial enzymes essentially fungal enzymes have been found applicable in various commercial production processes (Pinotti et al, 2020). Due to the importance associated with enzymes of microbial origin, selected microorganisms including bacteria, moulds, yeasts and insects have been globally studied for the biosynthesis of economically viable preparations of various enzymes for commercial applications (Chirumamilla et al, 2001;Jadhav et al, 2013;Behera et al, 2017;Banerjee et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Analytical study on Fungal Cellulase Produced by Penicillium Expansum grown on Malus Domestica (Apple Fruits)

Adedayo

Ayilara

2021

Nig Ann pure App Sci

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The rise in world industrialization and the cost of importing enzyme by local industries have led to arise in the search for novel and native enzyme producing microorganisms. Cellulase is an enzyme that catalyzes the breaking down of carbon chains in cellulose and hemicellulose, this research therefore aimed at studying fungal cellulase produced by Penicillium expansum grown on malus domestica (apple fruits). Fresh apple fruit was allowed to deteriorate under laboratory condition until there was visible mould growth. The mould with desired features of the organism of interest was subcultured by direct plating on PDA plates to which 10 % streptomycin has been added to prevent bacterial contaminants. The plates were incubated at 28±2 0C for 7 days until a visible mass of blue mycelia appear. The isolate was further subcultured onto freshly prepared media until pure culture was obtained. Characterization and identification of isolate were done using macroscopy and microscopy techniques. The isolate was re-inoculated into healthy apple fruits and the fruits were incubated at temperature of 28±2 oC for 8 days. Cellulolytic activity was examined every day throughout the incubation period. Crude enzyme was extracted each day using standard methods. Carboxyl methyl cellulose was used as standard for the crude cellulase activity assay after extraction from the infected apple fruits using Dinitrosalicylic acid (DNSA). Culture parameters like pH and temperature were also optimized to determine their effect on cellulolytic activity of the fungus. Cellulase activity was defined as the amount of glucose produced in μmol/mg/min under the assay condition. The highest cellulase activity of 86.84±0.52 μmol/mg/min was observed on day 6 of incubation at 28±2 oC and at pH 7. In conclusion, it is evident from this research that P. expansum isolated could be used as potential novel organism for industrial production of cellulase under optimized fermentation conditions.

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Section: Introductionmentioning

confidence: 99%

“…They are also used in animal feeds, in processing of fruit juice and in baking. Cellulases are also of immense importance in bioconversion of renewable organic biomass to usable biofuels and inorganic chemicals (Lynd et al, 2005;Imram et al, 2016;Sajith et al, 2016;Pinotti et al, 2020).…”

Section: Introductionmentioning

confidence: 99%