Evaluation of different lignocellulosic substrates for the production of cellulases and xylanases by the basidiomycete fungi Bjerkandera adusta and Pycnoporus sanguineus

Quiroz-Castañeda, Rosa Estela; Pérez-Mejía, Nancy; Martínez‐Anaya, Claudia; Acosta-Urdapilleta, L.; Folch-Mallol, Jorge Luis

doi:10.1007/s10532-010-9428-y

Cited by 44 publications

(31 citation statements)

References 28 publications

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“…Aspergillus terreus expresses four forms of endoglucanase when cultured in solid fermentation using rice straw as carbon source; on the other hand, just two forms of endoglucanase are secreted by the fungus when it was cultured on wheat bran and sugarcane bagasse [28]. In the same way, it was previously verified that a different strain of P. sanguineus (CEIBMD01) and Bjerkandera adusta (UAMH 8258) expressed different xylanase and endoglucanase profiles when cultured on different carbon sources [24]. This suggests that P. sanguineus UFV-Ps1 could produce different enzymatic profiles if it was cultured under different conditions.…”

Section: Analysis Of the Multienzymatic Complex By Gel Filtrationmentioning

confidence: 84%

“…The total protein secreted by fungus P. sanguineus after 5 days of fermentation was 0.125 mg mL −1 and the values of specific activities to all enzymatic activities measured are also presented in Table 1. P. sanguineus has been studied extensively in recent years due its extraordinary capacity to produce laccases and other ligninases [ (21,22]; however, there are few papers [23,24] highlighting the potential of this fungus to produce cellulases and hemicellulases. The production of cellulases by P. sanguineus achieved in this work was comparable to those described for the fungi T. reesei [25] and Penicillium funiculosum [26] cultivated under submerged conditions employing steam-pretreated spruce and pretreated sugarcane bagasse as carbon sources, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…1c) were very similar, indicating that P. sanguineus secretes at least five different forms of enzymes with the capacity to hydrolyze glucose-based β-linked oligosaccharides, in natural and synthetic substrates. The secretion of multiple forms of cellulases and hemicellulases with different properties is a typical characteristic of saprophytic fungi [11,24,27,28]. Several factors, including biotic and abiotic agents, are responsible to drive the expression of a specific enzyme or a specific group of enzymes; however, the carbon source is generally indicated as one of the most important factors.…”

Section: Analysis Of the Multienzymatic Complex By Gel Filtrationmentioning

confidence: 99%

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Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification

Falkoski

Guimarães

Almeida

et al. 2012

Appl Biochem Biotechnol

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The aim of this work was to evaluate the biochemical features of the white-rot fungi Pycnoporus sanguineus cellulolytic complex and its utilization to sugarcane bagasse hydrolysis. When cultivated under submerged fermentation using corn cobs as carbon source, P. sanguineus produced high FPase, endoglucanase, β-glucosidase, xylanase, mannanase, α-galactosidase, α-arabinofuranosidase, and polygalacturonase activities. Cellulase activities were characterized in relation to pH and temperature. β-Glucosidase and FPase activities were higher at 55 °C, pH 4.5, and endoglucanase activity was higher at 60 °C, in a pH range of 3.5-4.0. All cellulase activities were highly stable at 40 and 50 °C through 48 h of pre-incubation. Crude enzymatic extract from P. sanguineus was applied in a saccharification experiment using acid-treated and alkali-treated sugarcane bagasse as substrate, and the hydrolysis yields were compared to that obtained by a commercial cellulase preparation. Reducing sugar yields of 60.4% and 64.0% were reached when alkali-treated bagasse was hydrolyzed by P. sanguineus extract and commercial cellulase, respectively. Considering the glucose production, it was observed that P. sanguineus extract and commercial cellulase ensured yields of 22.6% and 36.5%, respectively. The saccharification of acid-treated bagasse was lower than that of alkali-treated bagasse regardless of the cellulolytic extract. The present work showed that P. sanguineus has a great potential as an enzyme producer for biomass saccharification.

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Section: Analysis Of the Multienzymatic Complex By Gel Filtrationmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Section: Analysis Of the Multienzymatic Complex By Gel Filtrationmentioning

confidence: 99%

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Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification

Falkoski

Guimarães

Almeida

et al. 2012

Appl Biochem Biotechnol

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“…Instead of using PDA as medium for fungal growth, lignocellulosic biomass has been added in medium as substrate for fungal growth. Rice husk as substrate in agar medium can increase the growth rate (1.24 cm/day) of PS at growth conditions of 28 °C for seven days [14]. As compared to this study, using PDA alone and by increasing the incubation temperature (30 °C ) can increase the growth rate up to 1.27 cm/day for five days.…”

Section: Results and Discussion Effect Of Different Agar Media On Gromentioning

confidence: 42%

“…It shows that 3.93% decreased of CDW when the Sc PS-7 was applied for seed culture production. However, the mycelia growth based on CDW of fungal biomass did not have direct correlation with production of cellulolytic enzymes [14]. It has been proven in this study for Sc PS-5.…”

Section: Effect Of Different Subculture Of Pycnoporus Sanguineus On Cmentioning

confidence: 45%

SEED CULTURE CONDITIONS FOR HIGH YIELD OF CELLULOLYTIC ENZYMES PRODUCTION FROM Pycnoporus sanguineus

Jalil

Kalil²,

Rahman³

et al. 2017

MJAS

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Low yields and productivity in fermentation industry usually due to poor quality of seed culture (inoculum). As the first stage in the fermentation process, inoculum consistency in terms of size and quality is clearly important for high yield of enzymes production. Selection of microorganism also plays an important role in improving the productivity. Fungi type of white rot basidiomycetes species is a well-known cellulolytic enzymes producer and capable to degrade many types of lignocellulosic biomass. Cellulolytic enzymes produced from white rot fungi, Pycnoporus sanguineus (PS) were investigated. The PS cultured on different agar media and parameter conditions of seed culture preparation in liquid medium broth were compared. Seed culture conditions of PS were influenced by many factors such as type of medium used for fungal growth, temperature, incubation time in liquid medium and subculture time on agar medium. PS was full-grown on potato dextrose agar (PDA) on day 5 compared to malt extract agar (MEA) on day 7. Seed culture conditions was determined using standard liquid medium, potato dextrose broth (PDB) at constant temperature (30 °C ), agitation speed (150 rpm) and pH (4.8) for duration of seven days using two different subculture of PS that were grown for five and seven days. It was found that the five days' subculture of PS shown 3.93% higher cell dry weight of fungal biomass (3.44 g/L on day 5) and higher cellulolytic enzymes activity; 95.49% (FPase), 3.14% (CMCase), 7.71% (exoglucanases) and 14.93% (xylanases). Maximum cellulolytic enzymes were found after 48 hours' incubation with filter paper activity (FPase) of 1.79 U/mL, carboxymethyl cellulase activity (CMCase) of 3.36 U/mL, exoglucanase activity (Avicelase) of 0.59 U/mL and xylanase enzymes activity of 0.66 U/mL. Reducing sugars concentration decreased from day 1 to 7 due to consumption of sugars for fungal growth. Seed culture conditions were strongly influenced by subculture and incubation time in liquid medium to produce high yield of cellulolytic enzymes from PS.Keywords: white rot fungi, Pycnoporus sanguineus, seed culture, cellulolytic enzymes, fermentation Abstrak Hasil dan produktiviti yang rendah dalam industri fermentasi biasanya disebabkan oleh kultur benih (inokulum) yang kurang berkualiti. Pada peringkat pertama proses fermentasi, konsistensi inokulum dari segi saiz dan kualiti sangat penting bagi pengeluaran enzim yang tinggi. Pemilihan mikroorganisma juga memainkan peranan penting dalam meningkatkan produktiviti. Jenis kulat pereput putih dari spesies basidiomycetes sangat terkenal sebagai pengeluar enzim selulolitik dan ia mampu untuk mengurai pelbagai jenis bahan lignoselulosa. Dalam kajian ini, enzim selulolitik dihasilkan daripada kulat pereput putih, Pycnoporus sanguineus (PS) yang dihidupkan di atas agar dan perbandingan parameter berbeza penyediaan kultur benih dalam medium cecair dikaji. Kultur benih PS dipengaruhi oleh pelbagai faktor seperti jenis medium yang digunakan untuk pertumbuhan kulat, suhu, masa pengera...

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Biocatalytic conversion of lignocellulose to platform chemicals

Jäger¹,

Büchs²

2012

Biotechnology Journal

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Naturally occurring lignocellulose can be used as a renewable resource for the sustainable production of platform chemicals that can in turn be converted to valuable fine chemicals, polymers, and fuels. The biocatalytic conversion of lignocellulose is a very promising approach due to its high selectivity, mild conditions, and low exergy loss. However, such biocatalytic processes are still seldom applied at the industrial scale since the single conversion steps (pretreatment, hydrolysis, and fermentation) may exhibit low conversion rates, low efficiencies, or high costs. The biocatalytic conversion of lignocellulose to platform chemicals is reviewed in this work. Structures and production rates of lignocellulose are described, and platform chemicals that may be produced from lignocellulose are summarized. Biocatalytic conversion of lignocellulose is distinguished from conventional non-selective approaches. All essential conversion steps used in biocatalytic approaches (pretreatment, hydrolysis, and fermentation) are reviewed in detail. Finally, potential interactions between these conversion steps are highlighted and the advantages as well as disadvantages of integrated process configurations are elucidated. In conclusion, a comprehensive understanding of the biocatalytic conversion of lignocellulose is provided in this review.

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Evaluation of different lignocellulosic substrates for the production of cellulases and xylanases by the basidiomycete fungi Bjerkandera adusta and Pycnoporus sanguineus

Cited by 44 publications

References 28 publications

Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification

Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification

SEED CULTURE CONDITIONS FOR HIGH YIELD OF CELLULOLYTIC ENZYMES PRODUCTION FROM Pycnoporus sanguineus

Biocatalytic conversion of lignocellulose to platform chemicals

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