Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

Abstract

This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1; the storage duration of ovaries were 6, 9 and 12 h. Experiment -2; the techniques for oocytes recovery were slicing, aspiration and slicing + aspiration. The ovaries were collected from slaughterhouse and kept in saline solution during transportation to laboratory. A total of 136 oocytes were used for experiment-1, and 246 oocytes for experiment-2. This study found that the number and quality of oocytes were significantly different (P<0.05) as the effect of storage time. The average number of oocytes were 15.90±2.48; 17.10±1.38 and 13.60±3.00, in 6 h; 9 h and 12 h of storage time, respectively. The techniques of oocytes collection significantly (P<0.05) affect the quantity of oocytes but no significant (P>0.05) on oocytes quality. The average of oocytes of groups slicing, aspiration and slicing plus aspiration were 14.66±2.09; 9.46±2.99 and 16.40±6.86, respectively. Both experiment was significant effect (P<0.05) on immaturation rate of bovine. Conclusion of this study the storage time of ovaries was suitable in 9 h and the technique of oocytes collection was in slicing plus aspiration.

Pengaruh Keberadaan Corpus Luteum Terhadap Kualitas Oosit dan Tingkat Maturasi Oosit Kerbau Secara In Vitro

“…Terdapat berbagai faktor yang mempengaruhi kualitas dan jumlah oosit diantaranya umur, metode koleksi dan status reproduksi (Udin et al, 2020). Pada hakikatnya oosit yang terdapat pada lingkungan folikel yang berada pada ovarium pertumbuhannya mengikuti siklus tertentu (Parera dan Lenda, 2019).…”

“…Oosit yang telah dimaturasi kemudian didenudasi sel -sel kumulusnya dengan cara dipipet berulang -ulang dalam medium PBS dan 2% BSA (Udin et al, 2020), setelah itu dibuat preparat dan difiksasi dalam larutan ethanol absolute dan asam asetat (3:1) selama 48 jam (Nanda, 2017). Setelah itu preparat diwarnai dengan 2% aceto orcein selama lima menit dan status inti oosit diamati di bawah mikroskop dengan pembesaran 40x10.…”

Effect of oocytes collection methods on oocytes quantity and quality in local Iraqi ewes

“…The procedures for collecting oocytes from the ovaries of slaughtered animals include slicing, ovary puncture and follicle aspiration (Wani et al, 2000). Because the use of these oocytes allows for the acquisition of a large number of ovaries at a minimal cost (Udin et al, 2020;Gharban and and Al-Shaeli, 2021). It's also critical to improve IVEP technology's efficiency in the development of embryos derived from slaughterhouse animals' ovaries, as well as procedures that promote the harvesting of a large number of high-quality oocytes per ovary (Mohammadi, 2010).…”