Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts

Klančnik, Anja; Piskernik, Saša; Jeršek, Barbara; Možina, Sonja Smole

doi:10.1016/j.mimet.2010.02.004

Cited by 506 publications

(380 citation statements)

References 27 publications

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“…In the other hand, T. lanceolatus ethanol extract was active on all strains tested contrary to A. sativum methanol extract where 2 mg/mL of this extract was ineffective against S. aureus and C. albicans [20] . Furthermore, inhibition zone diameters of C. sinensis ethanol extract were up to 16 mm, 17 mm, 17 mm against B. subtilis, E. coli, and S. aureus respectively, as the strongest antimicrobial activity [21] .…”

Section: Discussionmentioning

confidence: 97%

“…We have used Kirby-Bauer's agar disc diffusion modified method [16] , as preliminary antimicrobial potency evaluation of extracts. In short, plates of Mueller-Hinton agar for bacteria and Sabouraud agar for fungi were firstly inoculated by swabbing of standardized microbial suspension (10 8 CFU/ mL), accordingly to Clinical And Laboratory Standards Institute 2006 recommendations [15] .…”

Section: Disc Diffusion Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Benbelaïd

Khadir

Abdoune

et al. 2013

Asian Pacific Journal of Tropical Disease

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Section: Discussionmentioning

confidence: 97%

Section: Disc Diffusion Methodsmentioning

confidence: 99%

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Benbelaïd

Khadir

Abdoune

et al. 2013

Asian Pacific Journal of Tropical Disease

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“…Fusarium oxysporum, Botrytis cinerea and Alternaria alternata were obtained from the department's library. To evaluate the antifungal potential of the essential oils, the modified Agar Dilution method [14] . The mixtures were then poured into 5-cm-diameter Petri dishes.…”

Section: Evaluation Of the Antifungal Activity Of Essential Oilsmentioning

confidence: 99%

Essential Oils from the Leaves and Flowers of <i>Callistemon viminalis</i>: Chemical Characterization and Evaluation of the Insecticide and Antifungal Activities

Sales¹,

Cardoso²,

Guimarães³

et al. 2017

AJPS

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The chemical compositions of the essential oils from the leaves and flowers of Callistemon viminalis and their insecticide and fungitoxic activities were determined. The essential oil was extracted by the hydrodistillation method using a modified Clevenger apparatus. The chemical characterization was performed by GC-MS and GC-FID. The evaluation of the insecticidal activity was performed with the Myzus persicae aphid, and the antifungal potential was determined via the inhibition of the mycelial growth of Alternaria alternata, Fusarium oxysorum and Botrytis cinérea phytopathogenic fungi. The principal components of the essential oils from the leaves and flowers were eucalyptol (84.60% and 61.47%), α-pinene (10.28% and 21.48%) and α-terpineol (2.59% and 2.79%), respectively. The use of a 0.5% concentration of the essential oil from the flowers influenced the preference of aphids and their reproduction. The number of adult aphids decreased within a period of 48 hours in the presence of the essential oil from the leaves. In the test with no chance of choice, the mean number of adults decreased with both oils within 48 hours.

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“…Preliminary antimicrobial activity of AN, LN, and folded orange oils (5×, 10×, 20×) was tested against four foodborne pathogens, using disk diffusion technique (Klančnik, Piskernik, Jeršek, & Možina, 2010). An aliquot (100 μL) of bacterial suspension (adjusted to 0.5 McFarland ≈ 10 8 ) was distributed homogeneously on MHA plates.…”

Section: Antimicrobial Activity Assay (Disk Diffusion Assay)mentioning

confidence: 99%

“…Cultures were incubated at 37°C for 24 h. After that, bacterial survival was determined by plate counting. MIC was defined as the lowest concentration of oil that decreased growth about 90% of the bacterial population after 24 h of incubation, while the MBC was defined as the lowest concentration that completely inhibited growth on the MHA plate after 24 h of incubation (Klančnik et al, 2010). L. monocytogenes was tested the same way under the conditions referred above.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning

confidence: 99%

Chemical composition, antimicrobial, and antioxidant activities of orange essential oil and its concentrated oils

Torres-Alvarez

González

Rodríguez

et al. 2016

CyTA - Journal of Food

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In this study, the chemical composition, antimicrobial, and antioxidant activities of orange essential oil (AN), its folded orange oils (5×, 10×, 20×) and d-limonene (LN) were investigated. The results observed in the chemical composition showed a decrease in the major component LN, in contrast to other minor components, which increased their concentration, such as decanal, linalool, and α-terpineol. The antimicrobial activity was determined for foodborne pathogens using the disk diffusion method followed by the minimum inhibitory concentration and minimum bactericidal concentration. Results showed that folded orange oils (5×, 10×, 20×) had better antimicrobial activity than AN and LN. The antioxidant activity was carried out by 2,2ʹ-azinobis-3-ethylbenzthiazoline-6-sulphonate and 1,1-diphenyl-2-picrylhydrazyl methods; folded orange oil 20× presented significantly better results (p ≤ 0.05) than other oils studied. Using folded oils from AN could be a natural alternative in food processing as ingredients with antimicrobial and antioxidant effect.Perfil químico, actividad antimicrobiana y antioxidante del aceite esencial de naranja y sus aceites concentrados RESUMEN En este estudio, se investigó la composición química, actividad antimicrobiana y antioxidante del aceite esencial de naranja (AN), sus concentrados (5×, 10×, 20×) y d-limoneno (LN). Los resultados en la composición química mostraron una disminución del componente mayoritario d-limoneno, contrario a otros componentes minoritarios que aumentaron su concentración como es el decanal, linalol y α-terpinol. La actividad antimicrobiana fue determinada para bacterias de importancia en alimentos por el método de difusión en disco seguido de la concentración mínima inhibitoria (CMI) y concentración mínima bactericida (CMB). Los resultados mostraron que los aceites concentrados (5×, 10×, 20×) presentaron significativamente mayor actividad antimicrobiana que el AN y LN. La actividad antioxidante se realizó por los métodos de ABTS y DPPH obteniendo que la fracción 20×, presentó actividad antioxidante significativamente mayor (p ≤ 0,05) que los demás aceites estudiados. Los aceites concentrados del aceite esencial de naranja podrían ser una alternativa en la aplicación en alimentos como ingredientes con efecto antimicrobiano y antioxidante ARTICLE HISTORY

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Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts

Cited by 506 publications

References 27 publications

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Essential Oils from the Leaves and Flowers of <i>Callistemon viminalis</i>: Chemical Characterization and Evaluation of the Insecticide and Antifungal Activities

Chemical composition, antimicrobial, and antioxidant activities of orange essential oil and its concentrated oils

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Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts

Cited by 506 publications

References 27 publications

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Essential Oils from the Leaves and Flowers of &lt;i&gt;Callistemon viminalis&lt;/i&gt;: Chemical Characterization and Evaluation of the Insecticide and Antifungal Activities

Chemical composition, antimicrobial, and antioxidant activities of orange essential oil and its concentrated oils

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Product

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Essential Oils from the Leaves and Flowers of <i>Callistemon viminalis</i>: Chemical Characterization and Evaluation of the Insecticide and Antifungal Activities