Evaluation of Dose Dependent Effects of Gamma Radiation on Rat Tongue Internal Structures: Histomorphometric and Immunohistochemical Study

El-Haddad, Khaled; Metwaly, Hamdy

doi:10.21608/edj.2018.77388

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…Regarding the effects of gamma radiation on the lingual tissues, gamma radiation, particularly 6 Gy dose, produced defective changes in the interior structures of the tongue. The most noticeably affected tissues are the serous acini of the lingual minor salivary glands while the least affected tissues are the intrinsic muscles ( El-Haddad and Metwaly, 2018 ) .…”

Section: Introductionmentioning

confidence: 99%

Effects of dose-dependent response to gamma radiation on circumvallate papilla by expression of caspase-3 in vivo

El-Haddad

El-Faramawy

2021

The Saudi Dental Journal

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Background Radiotherapy is one of the most significant treatment modality of head and neck cancers. However, it has various hazards on the normal tissues in the radiation field. One of these affected tissues is the lingual mucosa with their papillae such as circumvallate papilla. The effects of radiation on the lingual specialized mucosa may be represented by radiation-induced mucositis and taste alteration including partial or complete loss of taste. Objectives The aim of the study was to evaluate the dose-dependent response of circumvallate papillae to gamma radiation by immunohistochemical expression of caspase-3. Material and methods Twenty-four adult male albino rats were divided into 3 equal groups irradiated at 2.0, 4.0 and 6.0 Gy whole-body gamma radiation doses. Six non-irradiated rats were used as the control group. The radiation effects on circumvallate papillae were evaluated three days after irradiation via histomorphometric investigation of the papillary size and taste buds’ distortion in addition to an immunohistochemical assessment of the apoptotic activity using Caspase-3 marker. Results Dose-related changes were observed in the circumvallate papillae size and morphology and taste buds affection. The changes were obviously detected in rats irradiated at 4 Gy and 6 Gy doses. The detection of caspase-3 marker was evident in a dose-dependent manner in all the irradiated groups, more noticeably in the taste bud cells. Conclusions It could be concluded that circumvallate papillae are adversely affected in a dose-dependent manner by gamma radiation particularly in 4 Gy and 6 Gy doses.

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Section: Introductionmentioning

confidence: 99%

Effects of dose-dependent response to gamma radiation on circumvallate papilla by expression of caspase-3 in vivo

El-Haddad

El-Faramawy

2021

The Saudi Dental Journal

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The efficacy of using metformin and/or quercetin for amelioration of gamma-irradiation induced tongue toxicity in diabetic rats

Ahmed,

Bakr,

Rasmy

2024

BMC Oral Health

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Background Diabetes is a common disease that cancer patients may suffer from and may aggravate side effects of radiotherapy. This study aimed to detect whether metformin and/or quercetin will improve gamma-irradiation induced tongue toxicity in diabetic rats. Methods 35 male albino rats were divided into five groups; NOR no streptozotocin, no radiation and no treatment was given, DR rats were subjected to streptozotocin then gamma-irradiation, DRM rats were subjected to streptozotocin then gamma-irradiation then metformin, DRQ rats were subjected to streptozotocin then gamma-irradiation then quercetin, DRMQ rats were subjected to streptozotocin then gamma-irradiation then metformin and quercetin. Rats were euthanized 24 h after last treatment dose. Mean blood glucose level was recorded. Tongue specimens were stained with H&E and CD68. Histomorphometric analysis of length, diameter and taste buds of lingual papillae and epithelial, keratin and lamina propria thickness and CD68 positive cells were calculated. Results Blood glucose level of DRMQ was significantly lower than DR, DRM and DRQ, whereas higher than NOR. Metformin or quercetin partially restored tongue structure, papillae length and diameter and tongue layers thickness. The ameliorative effect was superior when metformin and quercetin were used together. Diabetes and irradiation significantly increased number of CD68 positive macrophages in submucosa and muscles. Metformin or quercetin significantly reduced number of lingual macrophages with more noticeable effect for quercetin. Treatment with metformin and quercetin significantly decreased number of macrophages. Conclusions Combined use of metformin and quercetin might help mitigate the harmful effects of radiotherapy and diabetes on lingual tissues.

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Influence of Nanocurcumin and Photodynamic Therapy Using Nanocurcumin in Treatment of Rat Tongue Oral Squamous Cell Carcinoma Through Histological Examination and Gene Expression of BCL2 and Caspase-3

Abdelgawad¹,

Abdelaziz²,

El-Begawey³

et al. 2023

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Evaluation of Dose Dependent Effects of Gamma Radiation on Rat Tongue Internal Structures: Histomorphometric and Immunohistochemical Study

Cited by 3 publications

References 42 publications

Effects of dose-dependent response to gamma radiation on circumvallate papilla by expression of caspase-3 in vivo

Effects of dose-dependent response to gamma radiation on circumvallate papilla by expression of caspase-3 in vivo

The efficacy of using metformin and/or quercetin for amelioration of gamma-irradiation induced tongue toxicity in diabetic rats

Influence of Nanocurcumin and Photodynamic Therapy Using Nanocurcumin in Treatment of Rat Tongue Oral Squamous Cell Carcinoma Through Histological Examination and Gene Expression of BCL2 and Caspase-3

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