Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier

Abstract

In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery. The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined. The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro. The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype. Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo. Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.

Biological Role of Gellan Gum in Improving Scaffold Drug Delivery, Cell Adhesion Properties for Tissue Engineering Applications

“…In another study, GG/poloxamer-heparin (PoH) hydrogel was used as a carrier for bone marrow stem cell (BMSCs) delivery, in that experiment, they prepared a double network of GG hydrogel composed of PoH as a matrix platform for stem cell cultivation. They found that the hydrogel supported cell adhesion, distribution ( Figure 5), and ECM production in an in vivo model [65]. Shin et al [38] used GG/Silk fibroin (SF) for the chondrogenic differentiation ability of BMSCs, using miR-30.…”

“…Major routes for preparing gellan gum-based biomaterials[4,32,[34][35][36][37][38][39][65][66][67][68][69].…”

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

“…The DNH system was tested on bone marrow stem cells isolated from rabbits (rBMSCs) revealing that supported cell survival, maintained cell's morphology and phenotype. The in vivo results have demonstrated that PoH/GG DNH endorse the cell distribution, adherence, and ECM production [173].…”

Application of double network of gellan gum and pullulan for bone marrow stem cells differentiation towards chondrogenesis by controlling viscous substrates

“…The BMSCs were obtained following the previous reported study. (Choi et al, 2019) Briefly, the legs were extracted, and soft tissues were removed. The bones were rinsed with a 1.5% penicillin/streptomycin (PS, Gibco, Big Cabin, OK, USA) diluted in PBS.…”