Evaluation of Electrostatically Endothelial Cell Seeded Expanded Polytetrafluoroethylene Grafts in a Canine Femoral Artery Model

Fields, Charles; Cassano, Anthony D.; Makhoul, Raymond G.; Allen, Cynthia M.; Sims, Rick L.; Bulgrin, Jeffrey P.; Meyer, Andrew; Bowlin, Gary L.; Rittgers, Stanley E.

doi:10.1106/088532802030556

Cited by 29 publications

(19 citation statements)

References 23 publications

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“…41, 60 Numerous techniques have been developed since then with the goal of enhancing EC coverage and retention on synthetic grafts such as cell settling, 16,25,33 hydrostatic delivery, 28,56,61 electrostatic attraction, 7,15 and pre-coating/surface modification. 2,27,29,42,48,50 While these techniques have improved short-term graft performance with varying degrees of success, long-term EC retention remains problematic for some of these approaches.…”

Section: Introductionmentioning

confidence: 99%

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

2006

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It is generally accepted that endothelialization and subsequent development of a functional endothelium are of paramount importance to the success of any bioartificial artery. In this study, we aimed to assess the ability of smooth muscle cell-remodeled, fibrin-based media-equivalents (MEs) to be endothelialized, examine the morphological changes of endothelial cells (ECs) associated with exposure to physiologically-relevant shear stress in a custom-built bioreactor, and determine if adherent ECs are capable of withstanding average physiological shear stresses. It was found that MEs could be readily endothelialized with surface coverages of 98.8 +/- 0.9% after two days, and the ECs expressed von Willebrand factor. Furthermore, EC retention remained high (steady: 96.5 +/- 4.4%, pulsatile: 94.3 +/- 4.3%) under exposure to physiologically relevant shear stresses for 48 h. The results indicate that these MEs are conducive to generating an EC monolayer, with the ECs possessing adhesion strength sufficient to withstand physiological shear stress and maintain a normal phenotype.

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Section: Introductionmentioning

confidence: 99%

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

2006

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“…More importantly, this technique of cell seeding had a significant ( p < 0.001) impact on acute healing of the specimens, reducing the migration of SMCs and likely decreasing the hyperplastic response. 47 On the other hand, there have not been any studies demonstrating the short-and long-term effects of this method (important before clinical use), and the system should be tested on other scaffolds, including biodegradable constructs.…”

Section: Electrostatic Cell Seedingmentioning

confidence: 99%

Cell-Seeding Techniques in Vascular Tissue Engineering

Villalona

Udelsman

Duncan

et al. 2010

Tissue Engineering Part B: Reviews

184

136

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“…It is speculated by the authors that this increased cellular retention may also reduce the need for large numbers of seeded ECs initially. Combining the canine in vivo results presented in this paper with those results previously obtained in vitro and in vivo [4][5][6][7]14], the electrostatic EC seeding technique shows promise in terms of a clinically acceptable protocol which could provide the necessary conditions to improve long-term patency rates of small caliber vascular grafts. Additional long-term ( > 18 weeks) in vivo animal data and subsequent clinical trials would be required to completely discern the vascular graft patency and the hyperplastic response associated with small diameter graft implantation.…”

Section: Amentioning

confidence: 65%

Endothelial Cell Seeding of a 4-mm I.D. Polyurethane Vascular Graft

Fields¹,

Cassano

Allen

et al. 2002

J Biomater Appl

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We evaluated the extent (luminal coverage) of the endothelial cell (EC) lining/neointimal development and the thromboresistance of electrostatically EC seeded small diameter ChronoFlex-polyurethane vascular grafts. The evaluation consisted of harvesting autologous, canine jugular vein ECs, electrostatically seeding the polyurethane grafts (4-mm I.D., length = 6 cm) with the harvested ECs, implanting the grafts in a canine femoral artery model for four to six weeks, and excising the grafts for histological and scanning electron microscopy evaluations. Results of the histological evaluation (mid-graft region only) indicated that electrostatic EC seeding led to neointimal development and to minimal to no thrombus formation within the EC seeded grafts. The unseeded control grafts resulted in no neointimal development and substantial thrombus formation on the graft luminal surfaces. Scanning electron microscopy examination demonstrated a mature, confluent endothelium with a "cobblestone" appearance on the EC seeded graft luminal surface after six weeks. We conclude that electrostatic EC seeding enhanced the development of a neointima and reduced the incidence of thrombosis in polyurethane grafts implanted in a canine femoral artery model.

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Evaluation of Electrostatically Endothelial Cell Seeded Expanded Polytetrafluoroethylene Grafts in a Canine Femoral Artery Model

Cited by 29 publications

References 23 publications

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

Cell-Seeding Techniques in Vascular Tissue Engineering

Endothelial Cell Seeding of a 4-mm I.D. Polyurethane Vascular Graft

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