Evaluation of errors during susceptibility reporting of glycopeptide antibiotics for enterococcal isolates on sole basis of widely used disk diffusion test

Saini, Vikas; Goyal, Nisha; Singh, Narendra; Goswami, Meenakshi

doi:10.4081/hls.2022.10806

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…One limitation of this study is that the disc diffusion assay has been reported to be relatively insensitive and, in some cases, inaccurate [52]. In particular, the disc diffusion assay for vancomycin is prone to significant errors, prompting the CLSI recommendation that MIC testing follow disc diffusion screens to differentiate vancomycin-susceptible from vancomycin-intermediate isolates [31,53]. Similarly, the disc diffusion assay for colistin is error-prone due to poor diffusion into agar, and currently, no standard disc diffusion assay for colistin exists [54].…”

Section: Discussionmentioning

confidence: 99%

Synergistic Antimicrobial Effects of Ibuprofen Combined with Standard-of-Care Antibiotics against Cystic Fibrosis Pathogens

Chen,

Ilanga,

Simbassa

et al. 2023

Biomedicines

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Cystic fibrosis (CF) is a common life-shortening genetic disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Lungs of CF patients are often colonized or infected with microorganisms requiring frequent courses of antibiotics. Antibiotic-resistant bacterial infections have been a growing concern in CF patients. Chronic bacterial infections and concomitant airway inflammation damage the lungs, ultimately leading to respiratory failure. Several clinical trials have demonstrated that high-dose ibuprofen reduces the rate of pulmonary function decline in CF patients. This beneficial effect has been attributed to the anti-inflammatory properties of ibuprofen. Previously, we have confirmed that high-dose ibuprofen demonstrates antimicrobial activity against P. aeruginosa both in vitro and in vivo. However, no study has examined the antimicrobial effect of combining ibuprofen with standard-of-care antimicrobials. Here, we evaluated the possible synergistic activity of combinations of common nonsteroidal anti-inflammatory drugs (NSAIDs), namely, ibuprofen, naproxen, and aspirin, with commonly used antibiotics for CF patients. The drug combinations were screened against different CF clinical isolates. Antibiotics that demonstrated increased efficacy in the presence of ibuprofen were further tested for potential synergistic effects between these NSAIDS and antimicrobials. Finally, a survival analysis of a P. aeruginosa murine infection model was used to demonstrate the efficacy of the most potent combination identified in in vitro screening. Our results suggest that combinations of ibuprofen with commonly used antibiotics demonstrate synergistic antimicrobial activity against drug-resistant, clinical bacterial strains in vitro. The efficacy of the combination of ceftazidime and ibuprofen against resistant P. aeruginosa was demonstrated in an in vivo pneumonia model.

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Section: Discussionmentioning

confidence: 99%

Synergistic Antimicrobial Effects of Ibuprofen Combined with Standard-of-Care Antibiotics against Cystic Fibrosis Pathogens

Chen,

Ilanga,

Simbassa

et al. 2023

Biomedicines

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Evaluation of Antibiotics by Disk Diffusion and Minimum Inhibitory Concentration Breakpoints in Urinary Tract Infections

Qadir,

Rehman,

Nasir

et al. 2024

PJHS

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Antibiotic resistance (ABR) has made it more challenging to treat uropathogenic organisms. It is impossible to compromise antimicrobial susceptibility testing (AST), which is essential and has a significant impact on infection treatment strategies. Although labor-intensive and technically challenging for everyday laboratory use, the agar dilution technique is appropriate for monitoring and assessing novel antimicrobials. Objective: To determine the minimum inhibitory concentration (MIC), Agar dilution technique and disk diffusion as susceptibility test methodologies. Methods: This study was carried out at Khyber Girls Medical College (KGMC) Peshawar. Keeping in view the Clinical and Laboratory Standards Institute (CLSI) guidelines AST was executed. BIOMÉRIEUX® API® kits and gram staining were utilized for identification of bacteria. The disk diffusion was performed using Thermo ScientificTM OxoidTM antibiotic discs of Co-trimoxazole, Levofloxacin, Nitrofurantoin and Fosfomycin. The MIC and zone of inhibitions for disk diffusion were noted according to the CLSI protocol. Results: 158 culture positive samples were isolated out of 680 total received. Esherechia Coli (E. coli) (74.1%) being the most isolated organism. In comparison of disk diffusion and agar dilution, categorical agreement for Levofloxacin, Cotrimoxazole, Nitrofurantoin and Fosfomycin were (82.28%, 72.15 %, 87.97% and 82.28%) respectively. Kappa coefficients of (0.64, 0.43, 0.57 and 0.37) (p < 0.0001) were calculated for Levofloxacin, Co-trimoxazole, Nitrofurantoin, and Fosfomycin respectively, revealing considerable level of agreement for these antibiotics. Conclusions: It was concluded that Agar dilution is more precise than disk diffusion but being more labor intensive and technical. Disk diffusion can still produce significantly accurate results with less resource consumption.

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Evaluation of errors during susceptibility reporting of glycopeptide antibiotics for enterococcal isolates on sole basis of widely used disk diffusion test

Cited by 2 publications

References 18 publications

Synergistic Antimicrobial Effects of Ibuprofen Combined with Standard-of-Care Antibiotics against Cystic Fibrosis Pathogens

Synergistic Antimicrobial Effects of Ibuprofen Combined with Standard-of-Care Antibiotics against Cystic Fibrosis Pathogens

Evaluation of Antibiotics by Disk Diffusion and Minimum Inhibitory Concentration Breakpoints in Urinary Tract Infections

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