Evaluation of five screening tests licensed in Argentina for detection of hepatitis C virus antibodies

Ré, Viviana Elizabeth; Gallego, Sandra; Treviño, Elena; Barbás, Gabriela; Dominguez, Claudia X.; Elbarcha, Osvaldo; Bepre, Héctor; Contigiani, Marta Silvia

doi:10.1590/s0074-02762005000300016

Cited by 5 publications

(1 citation statement)

References 25 publications

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“…(22,23) HCV RNA detection as an early diagnostic means has some disadvantages including RNA instability and contamination problems. (24)(25)(26) However, the HCV antigen appears in the serum earlier than the HCV antibody by about 40 days, almost at the same time as the HCV RNA.…”

Section: Discussionmentioning

confidence: 99%

Characterization and Application of Monoclonal Antibody Against Hepatitis C Virus Nonstructual Protein Three

Shi

Xie²,

Shi³

et al. 2012

Hybridoma

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Developing assays for detecting HCV antigens could be beneficial because viral proteins appear earlier than antibodies and are more stable than RNA in the serum. Monoclonal antibody was prepared by immunization and cell fusion. Subclass, specificity, and efficiency of monoclonal antibodies were determined by ELISA. Epitope specificity of monoclonal antibodies was analyzed by ELISA additivity test. HCV antigen in serum of hepatitis patients was examined by double monoclonal antibody sandwich ELISA. Five hybridoma cell lines were screened and named HCV(1), HCV(2), HCV(3), HCV(4), and HCV(5). These five monoclonal antibodies had high specificity and efficiency. The additivity test showed that HCV(2), HCV(4), and HCV(5) recognized different epitopes, which can be matched in ELISA. Of 173 anti-HCV positive patients, 37 (21.4%) were positive for HCV antigen. Of 1498 anti-HCV negative patients, 10 (0.67%) were positive for HCV antigen. Fifty normal controls were negative for HCV antigen. HCV antigen detection had moderate agreement and correlation with HCV RNA detection (kappa=0.577, p<0.01; r=0.59, p<0.01). This result indicates that the monoclonal antibody against HCV NS(3) may be a potential diagnostic reagent, which would provide a foundation for developing a sandwich ELISA of HCV antigen detection.

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Section: Discussionmentioning

confidence: 99%

Characterization and Application of Monoclonal Antibody Against Hepatitis C Virus Nonstructual Protein Three

Shi

Xie²,

Shi³

et al. 2012

Hybridoma

View full text Add to dashboard Cite

show abstract

The serological markers of acute infection with hepatitis A, B, C, D, E and G viruses revisited

Pondé

2017

Arch Virol

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Viral hepatitis is a liver infection caused by one of the six hepatitis viruses: hepatitis A, B, C, D, E, and G virus (HAV to HEV and HGV). These agents differ in their biological, immunological, pathological and epidemiological characteristics. They cause infections that, when symptomatic, lead to clinical manifestations and laboratory findings that are not specific to a particular virus, often making differential diagnosis difficult, especially when no knowledge is available regarding the patient's medical history or the epidemiological background. A number of acute-phase serological markers, such as anti-HAV, anti-HBc, anti-HDV and anti-HEV IgM antibodies, are able to provide a clear indication of an infection caused by HAV, HBV, HDV or HEV. Anti-HCV antibodies and HGV/RNA are used for the diagnosis of HCV and HGV infections. The importance of each of these markers will be reviewed, and different factors that can interfere with the diagnosis of acute infections caused by these viruses will be described.

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Clinical application and analysis of hepatitis C virus NS3 antigen detection by ELISA in human serum

Xie

Wu²,

Huang³

et al. 2007

Chinese Medical Journal

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Evaluation of five screening tests licensed in Argentina for detection of hepatitis C virus antibodies

Abstract: Although the sensitivity of the assays is the first parameter to be considered for blood screening, more studies should be carried out to assess the specificity of such assays.

Cited by 5 publications

References 25 publications

Characterization and Application of Monoclonal Antibody Against Hepatitis C Virus Nonstructual Protein Three

Characterization and Application of Monoclonal Antibody Against Hepatitis C Virus Nonstructual Protein Three

The serological markers of acute infection with hepatitis A, B, C, D, E and G viruses revisited

Clinical application and analysis of hepatitis C virus NS3 antigen detection by ELISA in human serum

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