Evaluation of fixation time using Diff‐Quik for staining of canine mast cell tumor aspirates

Jackson, D E; Selting, Kimberly Anne; Spoor, Melanie S.; Henry, Carolyn J.; Wiedmeyer, Charles E.

doi:10.1111/vcp.12012

Cited by 15 publications

(9 citation statements)

References 5 publications

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“…20,21 However, this was not confirmed in a recent study. 22 In the present study, an accurate MCT diagnosis could not be made in 5% cases with RS. While this number is not high enough to discourage the use of RS in canine skin tumours cytology, practitioners should be aware that, in a minority of cases, RS may lead to a wrong or inconclusive diagnosis.…”

Section: Discussioncontrasting

confidence: 66%

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Comparison between May‐Grünwald‐Giemsa and rapid cytological stains in fine‐needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications

Sabattini

Renzi

Marconato

et al. 2018

Vet Comparative Oncology

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Mast cell tumours (MCTs) are often diagnosed by cytology based on the identification of purple intracytoplasmic granules with methanolic Romanowsky stains, including May-Grünwald-Giemsa (MGG). In clinical practice, aqueous rapid stains (RS) are commonly used, but mast cell granules may not stain properly. Aim of this prospective study was to investigate the frequency of MCT hypogranularity with RS and its potential implications in tumour identification, cytological grading assessment and recognition of nodal metastatic disease. Cytological preparations of canine primary MCTs and metastatic lymph nodes with subsequent histopathological confirmation were included. For each case, good-quality smears were stained with both MGG and RS and comparatively assessed. Eleven of 60 (18.3%) primary MCTs were hypogranular with RS; 9 of them were histologically high-grade tumours and in 3 cases (5%) a definitive MCT diagnosis could not be made. Accuracy in cytological grading assessment (85%) did not differ between RS and MGG. Thirteen of 28 (46.4%) metastatic lymph nodes were hypogranular with RS and 3 independent observers failed to identify nodal MCT metastases in 7% to 18% of RS-stained smears. This study confirms that, in limited cases, RS can be ineffective in staining MCT granules, particularly in high-grade tumours, thus making diagnosis more dependent on experience and quality of preparations. In dubious cases, methanolic stains should be applied. The use of RS is discouraged for the search of nodal metastases, as the identification of isolated mast cells can be more challenging.

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Section: Discussioncontrasting

confidence: 66%

“…It was suggested that more prolonged fixation (eg, minutes) would result in a more effective staining . However, this was not confirmed in a recent study …”

Section: Discussionmentioning

confidence: 99%

Comparison between May‐Grünwald‐Giemsa and rapid cytological stains in fine‐needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications

Sabattini

Renzi

Marconato

et al. 2018

Vet Comparative Oncology

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“…Romanowsky stains have advantages over Papanicolaou stains, including excellent depiction of cytoplasmic detail and superior visibility of bacteria, mucin and colloid, whilst limitations of Romanowsky stains include reduced nuclear and nucleolar detail (Jörundsson et al 1999 ). Mast cell granules are not always easily seen when using Diff-Quik (Jackson et al 2013 ).…”

Section: Stainingmentioning

confidence: 99%

Fine needle aspiration of abdominal organs: a review of current recommendations for achieving a diagnostic sample

Liffman

Courtman

2017

J of Small Animal Practice

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With the improved accessibility to ultrasound, fine needle aspiration of abdominal organs is now performed frequently in many veterinary practices. Despite this, there are many ‘unknowns’ as to the best method to produce a high‐quality sample. This report begins by highlighting recent literature on the risks and benefits of abdominal fine needle aspiration. It follows with recommendations about the equipment and method best suited to the procedure, including needle and syringe size and aspiration versus non‐aspiration techniques. Various smear preparations and laboratory submission requirements are also discussed. The final aspect of the review more specifically discusses fine needle aspiration of specific abdominal organs: the liver, kidney, spleen, pancreas, urinary bladder, prostate and abdominal lymph nodes.

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“…At the first inspection before the study initiation, a gingival, ear and dermal swab was done for each cat, rolled over a microscope slide and allowed to dry before staining with Diff-Quik stain (Mercedes Medical, Sarasota, FL, USA) (Jackson et al 2013).…”

Section: Clinical Evaluationmentioning

confidence: 99%

Clinical efficacy of nutraceutical diet for cats with clinical signs of cutaneus adverse food reaction (CAFR)

Mazzeranghi¹,

Zanotti²,

Cerbo³

et al. 2017

Polish Journal of Veterinary Sciences

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Food allergies and food intolerances are clinically difficult to discriminate. Most often, along with cutaneous adverse food reactions or CAFR, they are classified as adverse food reactions, whose causes are numerous, including toxic compounds.Eighteen indoor-housed domestic cats with evident clinical symptoms related to CAFR (drooling, back and neck intense itching, neck eczema, chronic conjunctivitis and stomatitis) involving skin lesions were studied. Cytological evaluations of ear, skin and gingival swabs revealed an increased turnover of keratinocytes while the oxytetracycline ELISA determination showed an unexpected high amount of oxytetracycline in all cats at the first visit. All cats were then randomly assigned to receive a standard (SD group) or a nutraceutical diet (ND group) for 60 days.In the ND group a significant reduction of the mean serum concentration of oxytetracycline, pruritus intensity and skin lesion severity (**p<0.01, ***p<0.001, and ***p<0.001, respectively) was observed after 60 days, and associated with a significant improvement in the clinical picture.Although a direct correlation between oxytetracycline presence within cat sera and CAFR-related symptoms has never been described, this study highlights the benefit of a specific nutraceutical diet supplementation in improving clinical symptoms and skin lesions in cats with CARF.

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Evaluation of fixation time using Diff‐Quik for staining of canine mast cell tumor aspirates

Abstract: Alteration in fixation time using Diff-Quik does not improve staining characteristics of mast cell tumors.

Cited by 15 publications

References 5 publications

Comparison between May‐Grünwald‐Giemsa and rapid cytological stains in fine‐needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications

Comparison between May‐Grünwald‐Giemsa and rapid cytological stains in fine‐needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications

Fine needle aspiration of abdominal organs: a review of current recommendations for achieving a diagnostic sample

Clinical efficacy of nutraceutical diet for cats with clinical signs of cutaneus adverse food reaction (CAFR)

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