Evaluation of four commercial transport media for the survival of Neisseria Gonorrhoeae

Arbique, Judy C.; Forward, Kevin R.; LeBlanc, John Randolph

doi:10.1016/s0732-8893(99)00134-0

Cited by 32 publications

(14 citation statements)

References 11 publications

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“…In this study, storage at low temperatures contributed to better performance of all swabs with transport medium, like previous reports [5,6,[16][17][18]. At room temperature, the metabolism of most bacteria is hasty, resulting in multiplication and negative variations in bacterial populations, like accumulation of bacterial toxins, pH changes and nutrient depletion.…”

Section: Discussionsupporting

confidence: 82%

Assessment of different storage conditions for Staphylococcus hyicus survival

Takeuti

Bernardi

Moreno

et al. 2018

J Infect Dev Ctries

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Introduction: Collecting swabs from skin lesions for bacteriological examination is frequently performed to the diagnosis of exudative epidermitis. This method is fast and non-invasive, but it depends directly on the viability of bacteria in clinical samples, which can be influenced by storage and shipment temperatures and the time of transportation. The aim of this study was to assess the capacity of four commercial transport media and swabs with no transport medium to preserve Staphylococcus hyicus (S. hyicus) for up to 10 days at room temperature and under refrigeration. Methodology: Samples were stored in swabs with no transport medium and four transport media (Amies, Amies with charcoal, Cary Blair and Stuart) for 10 days at room temperature and under refrigeration. Swabs were plated in Tween 80 Agar and colonies counted. Results: Samples kept in transport media showed better performance (P < 0.05) under refrigeration. Storage under refrigeration in Amies medium showed better results than all other transport media and swabs (P < 0.05). Amies medium and swabs with no transport medium showed comparable results in room temperature (P > 0.05). In additional, refrigerated Amies medium and swabs with no transport medium at room temperature showed high performance for up to nine and three storage days, respectively. Conclusions: The recovery of S. hyicus in samples stored in Amies medium under refrigeration was higher when compared to other transport media. In addition, swabs with no transport medium could also be indicated when samples are stored at room temperature within three days.

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Section: Discussionsupporting

confidence: 82%

Assessment of different storage conditions for Staphylococcus hyicus survival

Takeuti

Bernardi

Moreno

et al. 2018

J Infect Dev Ctries

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“…Owing to the off-site location of the laboratory, transport time was undoubtedly a contributing factor. Refrigeration of primary specimens may also have affected the yield of GC although some evidence suggests that GC may survive under these conditions 28. The sampling order (NAAT swabs first, followed by GC culture and CT culture) could have also theoretically disadvantaged the yield of culture, although there appeared to be more than adequate testing surface area available as the surface area of the swabs was quite small relative to the area being tested.…”

Section: Discussionmentioning

confidence: 99%

Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in pharyngeal and rectal specimens using the BD Probetec ET system, the Gen-Probe Aptima Combo 2 assay and culture

Ota

Tamari

Śmieja

et al. 2009

Sexually Transmitted Infections

105

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“…21, Table 2) and the other a reference strain (ATCC 19424, Table 2), both with an inoculum size of 1.4 ϫ 10 6 CFU/ml, were both recovered at 24 h, but only one remained viable at 48 h. In strains with lower inocula, 48-h viability was obtained with some strains with an inoculum size as low as 0.6 ϫ 10 5 CFU/ml, but not with others at even higher inocula. There was no consistency in yield within this group, suggesting that viability in some strains was strain dependent and not only contingent on the number of colonies on the swab, an observation noted by other investigators (1). While a few recent investigations of swab transport systems had tested a minimal number of gonococcal strains, often with a single strain (usually an ATCC strain), to study efficacy (6, 7, 12, 13; Hetchler et al, Abstr.…”

mentioning

confidence: 81%

“…The effects of duration of swab storage and of inoculum size on organism viability were also studied (Tables 2 and 3). Although all S. pneumoniae strains were recovered at all holding periods regardless of inoculum size, prolonged storage at ambient temperature reduced the yield of N. gonorrhoeae, a phenomenon universally observed (1,18). As early as 1954, Stuart and his colleagues had remarked that "Over 24 hours, the viability of gonococci deteriorates progressively."…”

mentioning

confidence: 86%

“…While swab systems are considered less optimal than direct plating for culturing purposes, they have become increasingly important in view of the delay of specimen transport necessitated by recent strategies of cost containment and consolidation of laboratory services. As a result, there have been continuous efforts to improve and assess the efficacies of swab systems and to develop industry-wide standards to monitor their performances (1,6,7,12,13,16,18; C. Hetchler, C. Brown, and J. C. Galbraith, Abstr. 100th Gen. Meet.…”

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confidence: 99%

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Efficacy of a Swab Transport System in Maintaining Viability of Neisseria gonorrhoeae and Streptococcus pneumoniae

2001

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The efficacy of swab transport systems in maintaining viability of Neisseria gonorrhoeae and Streptococcus pneumoniae is crucial both for establishing definitive diagnosis and for monitoring emerging resistance. We tested the efficacy of a newly modified Amies charcoal swab transport system, the StarSwab SP131X (Starplex Scientific, Inc., Etobicoke, Ontario, Canada), by using a combined total of 31 clinical and American Type Culture Collection stock reference strains of N. gonorrhoeae and S. pneumoniae in 46 suspensions of concentrations ranging from 10 5 to 10 8 CFU/ml. Triplicate swabs per strain held at room temperature for 0, 24, and 48 h were plated without prior vortexing, and their growths were graded. All 31 strains were viable at 0 and 24 h. Gonococcal viability at 48 h varied considerably, even among strains with comparable inoculum sizes, suggesting that viability might be strain dependent and confirming the different structural and growth profiles of gonococcal strains. S. pneumoniae strains showed consistent viability, with all strains recovered at all holding periods. This study demonstrates that the StarSwab SP131X is capable of maintaining the viability of N. gonorrhoeae and S. pneumoniae for at least 24 and 48 h, respectively, and reinforces the need for adequate sampling and for timely processing of specimens to maintain optimum performance.

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Evaluation of four commercial transport media for the survival of Neisseria Gonorrhoeae

Cited by 32 publications

References 11 publications

Assessment of different storage conditions for Staphylococcus hyicus survival

Assessment of different storage conditions for Staphylococcus hyicus survival

Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in pharyngeal and rectal specimens using the BD Probetec ET system, the Gen-Probe Aptima Combo 2 assay and culture

Efficacy of a Swab Transport System in Maintaining Viability of Neisseria gonorrhoeae and Streptococcus pneumoniae

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