Evaluation of four rapid methods for hemoglobin screening of whole blood donors in mobile collection settings

Gómez-Simón, Antonia; Navarro-Núñez, Leyre; Pérez‐Ceballos, Elena; Lozano, Marı́a Luisa; Candela, M. J.; Cascales, Almudena; Martı́nez, Constantino; Corral, Javier; Vicente, Vicente; Rivera, José

doi:10.1016/j.transci.2007.01.010

Cited by 43 publications

(46 citation statements)

References 16 publications

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“…Notwithstanding its simplicity, our paper-based Hb assay demonstrated excellent comparison (SD = 0.62 g/dL, n = 54) with the Medonic hematology analyzer. The performance of our assay also compares favorably with conventional POC devices (14, 19, 37), particularly in the context of our target applications: (a) providing the measurement of [Hb] when no other methods are available, and (b) enabling public health screening in resource-limited settings where the lowest cost, rather than the highest accuracy, is most important.…”

Section: Discussionmentioning

confidence: 95%

Simple Paper-Based Test for Measuring Blood Hemoglobin Concentration in Resource-Limited Settings

et al. 2013

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Background The measurement of hemoglobin concentration ([Hb]) is performed routinely as a part of a complete blood cell count to evaluate the oxygen-carrying capacity of blood. Devices currently available to physicians and clinical laboratories for measuring [Hb] are accurate, operate on small samples and provide results rapidly, but may be prohibitively expensive for resource-limited settings. The unavailability of accurate but inexpensive diagnostic tools often precludes proper diagnosis of anemia in low-income developing countries. Therefore, we developed a simple paper-based assay for measuring [Hb]. Methods A 20-μL droplet of a mixture of blood and Drabkin’s reagent was deposited onto patterned chromatography paper. The resulting blood stain was digitized with a portable scanner and analyzed. The mean color intensity of the blood stain was used to quantify [Hb]. We compared the performance of the paper-based Hb assay with a hematology analyzer (comparison method) using blood samples from 54 subjects. Results The values of [Hb] measured using the paper-based assay and the comparison method were highly correlated (R2 = 0.9598); the standard deviation of the difference between the two measurements was 0.62 g/dL. The assay was accurate within 1 g/dL 90.7% of the time, overestimating [Hb] by ≥1 g/dL in 1.9% and underestimating [Hb] by ≥1 g/dL in 7.4% of the subjects. Conclusions This study demonstrates the feasibility of the paper-based Hb assay. This simple, low-cost test should be useful for diagnosing anemia in resource-limited settings, particularly in the context of care for malaria, HIV and sickle cell disease patients in sub-Saharan Africa.

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Section: Discussionmentioning

confidence: 95%

Simple Paper-Based Test for Measuring Blood Hemoglobin Concentration in Resource-Limited Settings

et al. 2013

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“…On the other hand, there was a good correlation between venous HemoCue values and cell counter values, questioning the reliability of capillary blood. Gómez-Simón et al59 evaluated the performance of three PHs (HemoCue, STAT-Site MHgb and CompoLab HB system) and attributed the inaccuracy in their performance mainly to the use of capillary blood.…”

Section: Methods For Hb Estimation In Blood Donorsmentioning

confidence: 99%

Techniques used for the screening of hemoglobin levels in blood donors: current insights and future directions

Chaudhary¹,

Sonker²

2017

JBM

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Blood donor hemoglobin (Hb) estimation is an important donation test that is performed prior to blood donation. It serves the dual purpose of protecting the donors’ health against anemia and ensuring good quality of blood components, which has an implication on recipients’ health. Diverse cutoff criteria have been defined world over depending on population characteristics; however, no testing methodology and sample requirement have been specified for Hb screening. Besides the technique, there are several physiological and methodological factors that affect accuracy and reliability of Hb estimation. These include the anatomical source of blood sample, posture of the donor, timing of sample and several other biological factors. Qualitative copper sulfate gravimetric method has been the archaic time-tested method that is still used in resource-constrained settings. Portable hemoglobinometers are modern quantitative devices that have been further modified to reagent-free cuvettes. Furthermore, noninvasive spectrophotometry was introduced, mitigating pain to blood donor and eliminating risk of infection. Notwithstanding a tremendous evolution in terms of ease of operation, accuracy, mobility, rapidity and cost, a component of inherent variability persists, which may partly be attributed to pre-analytical variables. Hence, blood centers should pay due attention to validation of test methodology, competency of operating staff and regular proficiency testing of the outputs. In this article, we have reviewed various regulatory guidelines, described the variables that affect the measurements and compared the validated technologies for Hb screening of blood donors along with enumeration of their merits and limitations.

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“…In comparison to these devices, 13,14 the HemoCue ® shows the highest level of agreement with automated haematology analysers, with a reported correlation of 99% when used by trained operators. 15 It complies with the International Committee on Standardization in Haematology's standards for haemoglobin measurement (ICSH, 1996).…”

Section: Introductionmentioning

confidence: 97%

Point-of-care estimation of haemoglobin concentration in all age groups in clinical practice

Schapkaitz

Mahlangu

2011

South African Family Practice

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Evaluation of four rapid methods for hemoglobin screening of whole blood donors in mobile collection settings

Cited by 43 publications

References 16 publications

Simple Paper-Based Test for Measuring Blood Hemoglobin Concentration in Resource-Limited Settings

Simple Paper-Based Test for Measuring Blood Hemoglobin Concentration in Resource-Limited Settings

Techniques used for the screening of hemoglobin levels in blood donors: current insights and future directions

Point-of-care estimation of haemoglobin concentration in all age groups in clinical practice

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