Evaluation of genetic variability in Symplocos laurina Wall. from two biodiversity hotspots of India

Bhagwat, Rasika M.; Banu, Sofia; Dholakia, Bhushan B.; Kadoo, Narendra Y.; Lagu, M. D.; Gupta, Vidya S.

doi:10.1007/s00606-014-1046-4

Cited by 8 publications

(3 citation statements)

References 35 publications

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“…Undoubtedly, SSR and RAPD dendrogram obtained by UPGMA analysis shows a location based clustering pattern suggesting the existence of intra-specific diversity (44,18,45). Literature survey reveals that RAPD is inconsistent and not reproducible though it amplifies both coding and non-coding regions of the genome.…”

Section: Resultsmentioning

confidence: 99%

Assessment of genetic diversity in five populations of Solanum torvum Swartz. from Tripura using SSR and RAPD markers

2020

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Polymerase Chain reaction (PCR) based molecular characterization has been undertaken for assessing the genetic diversity in five populations of Solanum torvum using SSR and RAPD markers. In this study, 8 SSRs produced 151 fragments of which 131 bands were polymorphic (86.38%). The primers, At5 amplified the highest number of polymorphic loci (27) and the highest PIC was recorded in CBT08 (0.54). In comparison, RAPD assay produced 70 bands with 79.16% polymorphism. The PIC value was highest in OPC14 (0.41). UPGMA clustering for SSR and RAPD markers grouped all the populations into two clusters. Our findings on SSR profile suggests that though different populations of S. torvum are inherited from a common ancestor eventually the population (STP5) with greater genetic diversity is stabilized in the high altitude of Sub - Himalayan region of Tripura in the due course of evolution.

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Section: Resultsmentioning

confidence: 99%

Assessment of genetic diversity in five populations of Solanum torvum Swartz. from Tripura using SSR and RAPD markers

2020

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“…(Peakall and Smouse 2012). The Polymorphism Information Content (PIC) was calculated using the formula 1 − p 2 − q 2 , where q is the frequency of no bands and p is the frequency of present bands (Rajwade et al 2010;Bhagwat et al 2014). The primer index (SPI) was then calculated using loci amplified by the same primer with summing up the PIC values (Bhagwat et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The Polymorphism Information Content (PIC) was calculated using the formula 1 − p 2 − q 2 , where q is the frequency of no bands and p is the frequency of present bands (Rajwade et al 2010;Bhagwat et al 2014). The primer index (SPI) was then calculated using loci amplified by the same primer with summing up the PIC values (Bhagwat et al 2014). The Marker Index (MI) for each primer was calculated as a product of PIC and effective multiplex ratio (EMR) (Zhang et al 2018); MI = EMR × PIC (Pecina-Quintero et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity of Saraca asoca (Roxb.) De Wilde: a commercially important, but endangered, forest tree species in Western Ghats, India

Saini

Hegde

et al. 2018

N.Z. j. of For. Sci.

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Background: Saraca asoca (Roxb.) De Wilde is a valuable tree used in traditional medicine against a variety of ailments. Almost all parts of the tree are used for various commercial herbal preparations. Due to overexploitation, the species is rapidly disappearing from Western Ghats where it is native and grew extensively until recently. Conservation of this important medicinal plant is therefore an urgent need. To plan effective conservation strategies, a scientific assessment of the genetic diversity and distribution is needed. Methods: Random Amplified Polymorphic DNA fingerprinting was employed and the population genetic structure of seven wild and three cultivated populations totalling 160 individuals of S. asoca in the Western Ghats region of Karnataka, Maharashtra and Goa for analysis. Results: Variation of 89% was observed within populations while 11% was observed among the populations of S. asoca. Gene flow (Nm) of 2.01 was observed, and 0.19 was the degree of genetic differentiation recorded. Unweighted Pair Group Method with Arithmetic average (UPGMA) cluster analysis generated a dendrogram that showed an admixture of all genotypes but with two major clusters, which was also supported by a STRUCTURE-based Bayesian model. One wild population was well, but inexplicably, differentiated from the rest. Conclusions: The study shows that there is still considerable genetic diversity existing in natural populations of S. asoca, suggesting good natural cross-pollination, giving encouraging indications that the gene pool is under no immediate threat. Any conservation strategy should utilise the observed genetic variation in the choice of planting stock for programmes of conservation, propagation and reforestation.

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Diversification and biogeography of Dawkinsia (Teleostei: Cyprinidae) in the Western Ghats-Sri Lanka biodiversity hotspot

et al. 2021

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Evaluation of genetic variability in Symplocos laurina Wall. from two biodiversity hotspots of India

Cited by 8 publications

References 35 publications

Assessment of genetic diversity in five populations of Solanum torvum Swartz. from Tripura using SSR and RAPD markers

Assessment of genetic diversity in five populations of Solanum torvum Swartz. from Tripura using SSR and RAPD markers

Assessment of genetic diversity of Saraca asoca (Roxb.) De Wilde: a commercially important, but endangered, forest tree species in Western Ghats, India

Diversification and biogeography of Dawkinsia (Teleostei: Cyprinidae) in the Western Ghats-Sri Lanka biodiversity hotspot

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