Evaluation of haploidization efficiency in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) through anther culture

Kurtar, Ertan Sait; Balkaya, Ahmet; Kandemir, Dilek

doi:10.1007/s11240-016-1074-6

Cited by 26 publications

(16 citation statements)

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“…The different responses to haploid induction reflect differences between species as well as differences among genotypes of an individual species. For example, genotype proved to be a key factor influencing in vitro gynogenesis and androgenesis in summer squash (Cucurbita pepo), cucumber (Cucumis sativus), winter squash (Cucurbita maxima), pumpkin (Cucurbita moschata), and watermelon (Citrullus lanatus) 13,[15][16][17] . A previous study of Gerbera jamesonii including ten different Gerbera genotypes showed that genotype affected callus induction, which ranged from 5% to 43%, as well as the shoot regeneration rate, which ranged from 57% to 95% 18 .…”

Section: Discussionmentioning

confidence: 99%

Haploid induction via unpollinated ovule culture in Gerbera hybrida

Fan

Cheng

Zhao

et al. 2020

Sci Rep

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Ovule-derived haploid culture is an effective and important method for genetic study and plant breeding. Gerbera hybrida is a highly heterozygous species, and the lack of homozygous lines presents a challenge for molecular genetic research. Therefore, we performed haploid induction through unpollinated ovule culture and evaluated the effects of several important factors on this culturing procedure in G. hybrida, including genotype, low temperature, and the development seasons of the ovules. Among 45 G. hybrida cultivars analyzed, 29 cultivars exhibited adventitious bud induction via in vitro unpollinated ovule culture with significant different responses, indicating that the genotype of donor plants was a vital factor for inducibility. Four cultivars with significantly different induction rates, including one non-induced cultivar, were selected to analyze seasonal effects. Ovules extracted in the summer consistently had the highest induction rates, and even the non-induced cultivar included in the analysis could be induced at low levels when ovules from summer were used. Low temperature treatment could also promote adventitious bud induction, and in particular, a strong and significant effect was detected after 7 days of cold treatment. Ploidy level measurements by flow cytometry revealed that 288 ovule-derived regenerants were haploid (55.17%) and 218 lines were diploid (41.76%). Moreover, genetic stability analysis of the regenerants indicated 100% similarity to the marker profile of the mother plant. This is the first report of ovule-derived haploids in G. hybrida, which may facilitate the development of homozygous lines for molecular research and plant breeding. Gerbera hybrida is one of the most popular ornamental plants worldwide 1. Since most of the commecial cultivars were bred from the crossing of wild-type G. jamesonii and G. viridifolia, the genome of G. hybrida is highly heterozygous 2. Hundreds of G. hybrida varieties with extremely rich flower color patterns are available in the flower market, including white, yellow, red, pink, purple, and brown, and G. hybrida ranks fourth in cut flowers after rose, chrysanthemum, and tulip 3. As a highly heterozygous species, G. hybrida naturally harbors genetic diversity, which is beneficial for hybridization breeding. However, the lack of a homozygous genome is challenging for molecular genetic research, including forward genetic screening and mutation mapping. Ploidy breeding, the modification of the number of chromosome sets in a plant genome, is frequently used in ornamental plant breeding to induce novel variation and to create homogenous lines 4. A commonly used protocol for ploidy breeding is haploid induction, which refers to haploid regeneration via a single gamete cell under specific conditions, using either pollen (anther or microspore culture) or egg cells (ovule culture) 5. Essentially, the applicability of haploid induction is the main trigger for ornamental breeding, and some haploids of ornamentals have been commercially developed, including the ...

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Section: Discussionmentioning

confidence: 99%

Haploid induction via unpollinated ovule culture in Gerbera hybrida

Fan

Cheng

Zhao

et al. 2020

Sci Rep

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“…Plant material and preparation of anti-mitotic agents. The ploidy levels of all plantlets of winter squash and pumpkin breeding lines derived from anther culture were checked by root tip chromosome counting [Kurtar et al 2016]. Nodal explants of haploid plantlets, having a leaf and a node, were transferred to modified solid E20A medium with the addition of 0.1 mg l -1 IAA and 0.1 mg l -1 IBA for micropropagation.…”

Section: Methodsmentioning

confidence: 99%

“…The plants were grown in controlled greenhouse then were transferred to the open field conditions. Ploidy status of treated plants was identified via chromosome counting in root tips as described by Kurtar et al [2016]. A plantlet for each Magenta box and ten Magenta boxes for three replicates (totally 30 plantlets) were cultured for each treatment.…”

Section: Methodsmentioning

confidence: 99%

“…In Cucurbitaceous, irradiated pollen technique is the efficient method to generate the genetically uniform DH's in melon [Sari et al 2010] and cucumber [Lotfi et al 1999], but this technique has not yet been fully optimized for production of high quantity haploids in squash [Kurtar et al 2002], pumpkin [Kurtar et al 2009] and winter squash [Kurtar and Balkaya 2010]. Thus, the alternative method, we conducted to anther culture with the aim of developing high quantity DH's in winter squash and pumpkin [Kurtar et al 2016].…”

Section: Introductionmentioning

confidence: 99%

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THE EFFECTS OF ANTI-MITOTIC AGENTS ON DIHAPLOIDIZATION AND FERTILITY IN WINTER SQUASH (Cucurbita maxima Duch.) AND PUMPKIN (Cucurbita moschata Duch.) ANDROGENIC HAPLOIDS

Kurtar¹

2018

Acta Sci.Pol.Hortorum Cultus

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Double-haploidization (DH) is one of the favorable techniques to obtain 100% pure double haploid plants (DH's) for generating the new F 1 cultivars in a short time. The fecundity of this technique depends on the high quantity of haploids and also fertile DH's. However, there are no comprehensive reports on the chromosome doubling and fertility (fruit and seed-set) of winter squash and pumpkin haploids, currently. Thus, to obtain high frequency and fertile DH's, the efficiency of different anti-mitotic agents (colchicine, amiprophos methyl, trifluralin and oryzalin) was tested at various concentrations and exposure times for both in vitro and in vivo conditions. Haploid plantlets recovered from anther cultures of winter squash and pumpkin lines were used for DH program. The haploid plants were wholly immersed in aqueous solutions of antimitotic agents in vitro and apical parts of haploid plantlets were treated with anti-mitotic agents three times in vivo. Since some plants remained haploid, and the highest DH efficiency was obtained from multiple treatments of colchicine to shoot tips of haploid plants in the rate of 93.3%. In vivo multiple treatments of 1% colchicine for an hour was found to be the best doubling procedure for the recovery of high-frequency fertile DH's in our winter squash and pumpkin breeding program.

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“…Kumar et al, 2003; AshokKumar and Murthy, 2004;Song et al, 2007;Zhan et al, 2009;Rakha et al, 2012;Hamidvand et al, 2013; Abdollahi et al, 2015Abdollahi et al, , 2016Kurtar et al, 2016;Asadi et al, 2018;Amirian et al, 2019). Однако выход растений-регенерантов остается достаточно низким и очень сильно зависит от генотипа донорного растения(Abdollahi et al, 2016;Kumar & Murthy, 2004;Kumar et al, 2003;Song et al, 2007) Asadi et al, 2018…”

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Production of Doubled Haploids in cucumber

et al. 2019

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Implementation of cell technologies has essentially improved the plant breeding process in agricultural crops in the world. The production of pure lines in cultivated crops, particularly among cross-pollinated species such as cucumber (Cucumis sativus L.) requires much time, labor and expense. Thus, the use of DH-plants for production of fully homozygous lines for one year becomes a very promising method for near cucumber breeding program. The major factor limiting the wide use of DH is a lack of effective protocol for large-scale plant production. In this review the historical facts with description of three main methods of DH-plant production were presented. By now these three methods have been such as parthenogenesis in situ induced by pollination with irradiated or chemically treated pollen; androgenesis in vitro including anther and isolated microspore cultivation in vitro; gynogenesis through ovule cultivation in vitro. Comparative analysis of published data with regard to the efficiency of the technology for DH-plant production was shown as well as advantages and limitations of each technology were described.

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Evaluation of haploidization efficiency in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) through anther culture

Cited by 26 publications

References 24 publications

Haploid induction via unpollinated ovule culture in Gerbera hybrida

Haploid induction via unpollinated ovule culture in Gerbera hybrida

THE EFFECTS OF ANTI-MITOTIC AGENTS ON DIHAPLOIDIZATION AND FERTILITY IN WINTER SQUASH (Cucurbita maxima Duch.) AND PUMPKIN (Cucurbita moschata Duch.) ANDROGENIC HAPLOIDS

Production of Doubled Haploids in cucumber

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