EVALUATION OF HBV DNA LEVELS IN SALIVA IN SUBJECTS WITH DIFFERENT VIREMIA AS WELL AS DURING PEGINTERFERON α-2a THERAPY

Krasteva, Assya; Panov, Vladimir; Jelev, Dejan; Antonov, Krasimir; Ivanova, Aneta; Krastev, Zahariy

doi:10.5272/jimab.2013194.355

Cited by 1 publication

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“…The average viral DNA cp/mL of the set of specimens was determined to be 2.66e11 in the saliva, 4.69e5 in the serum, and 3e4 in the cerumen. These results correlate with the results obtained by Krasteva et al in 2013 ( 27 ), who studied the saliva of 19 patients who had been receiving peg-interferon as a treatment for three months. The results indicated that all patients had HBV-DNA present in their serum samples; the load varied between 494 to 6.3e9 cp/mL.…”

Section: Discussionsupporting

confidence: 91%

“…However, in those whose serum viremia was higher than the 10 4 cp/mL, the viral load was reduced significantly ( 25 ). The difference between the obtained results from the current and the latter study may be related to the intake of peg-interferon ( 23 , 27 ). In this group, the increase in serum viral DNA load of HBeAg negative patients was accompanied by a decrease in salivary loads and an increase in cerumen loads ( Table 4 ).…”

Section: Discussioncontrasting

confidence: 70%

“…According to prior studies, HBs and HBe antigens are present both in the serum and in the saliva of the patients, while HBV-DNA may be found in their serum, cerumen, and saliva ( 24 - 27 ). Zhevachevsky et al (2000) performed a study on saliva gathered from 505 patients known to be infected with HBV.…”

Section: Discussionmentioning

confidence: 98%

“…In 2013, Eftekharian et al performed a study on the serum and cerumen of 30 patients who were all positive for HBsAg. In their study, HBV-DNA was extracted from the cerumen samples gathered from two subjects (6.6%) by employing PCR ( 29 ). The main difference between the current study and this mentioned research rests in the number of subjects and also in the method of extraction.…”

Section: Discussionmentioning

confidence: 99%

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Comparing HBV Viral Load in Serum, Cerumen, and Saliva and Correlation With HBeAg Serum Status in Patients With Chronic Hepatitis B Infection

et al. 2016

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BackgroundHepatitis B is a disease that is prevalent worldwide and is responsible for 10% of the deaths that occur every year. The virus persists in 5% of infected adults and 90% of infected children and can cause chronic hepatitis. In addition to blood, the virus may also be present in other secretions. Transmission through saliva, sexual fluids, and urine has also been confirmed.ObjectivesThe main aim of this study was to compare viral DNA copies in the serum, cerumen, and saliva of patients with HBeAg levels in their sera.Patients and MethodsThis was a cross-sectional study and subjects were selected by non-randomized methods. Serum, cerumen, and saliva samples were collected from 50 patients who were diagnosed with chronic hepatitis B about a year prior to the study. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the presence of HBsAg and HBeAg in the gathered specimens. Viral DNA was extracted from specimens by using a Qiagen kit. The number of viral DNA copies was determined using a real-time polymerase chain reaction (PCR) assay. The study was performed in Ilam province in western Iran.ResultsTwenty-eight percent of the patients were HBeAg positive. The average number of viral copies in serum, cerumen, and saliva was higher in women than in men, and a significant correlation was observed between the gender and average viral copies. However, no significant correlation was observed between viral copies present in the serum and cerumen with the age and gender of patients. In addition, no correlation was observed between serum HBeAg and viral copies present in serum, cerumen, and saliva. The correlation analysis confirmed a direct and definite correlation between viral DNA loads in the patients’ serum and cerumen.ConclusionsA significant direct correlation was observed between the viral DNA copies present in patients’ cerumen and serum. However, the correlation between saliva viral load with serum and cerumen viral load was very low and inverse. These findings suggest that the presence of the hepatitis B virus (HBV) in non-invasive specimens (such as cerumen and saliva) should also be evaluated when monitoring patients to determine the course of infection and disease.

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Section: Discussionsupporting

confidence: 91%