Evaluation of High-Resolution Typing Methods for Chlamydia trachomatis in Samples from Heterosexual Couples

Bom, Reinier J. M.; Christerson, Linus; Loeff, Maarten F. Schim van der; Coutinho, Roel A.; Herrmann, Björn; Bruisten, Sylvia M.

doi:10.1128/jcm.00128-11

Cited by 76 publications

(120 citation statements)

References 19 publications

Supporting

Mentioning

114

Contrasting

Order By: Relevance

“…Although high-resolution genotyping methods, such as multilocus variable number of tandem repeat analysis (MLVA), multilocus sequence typing (MLST) or microarray assay (Christerson et al, 2011;Ruettger et al, 2011;Bom et al, 2011;Wang et al 2011;Satoh et al, 2014;Hermann et al, 2015), have been recently described, their use in routine diagnostic practice is largely restricted by their complexity and high costs. So far these assays have basically played a role in epidemiological and phylogenetic analysis or in the evolutionary surveillance of specific clones, but what is really needed in the near future is the advent of rapid and cost-saving methods for faster and reliable management of patients (Pedersen et al, 2009;Xia & Xiong, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The gene encoding the major outer membrane protein (omp1), is widely used as a molecular target, since it contains four spaced variable domains (VDI to VDIV) flanked and interspaced by five conserved domains (Bom et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Genotyping of CT strains within a population may provide useful information on the pathogenesis of the infection and enables associations with clinical and epidemiological findings (Lagerga rd et al, 2010;Bom et al, 2011). Furthermore, genotyping can allow monitoring of contact tracing or evaluation of treatment success (Kapil et al, 2015), and may play a role in developing strategies for vaccine design (Geisler et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chlamydia trachomatis infection prevalence and serovar distribution in a high-density urban area in the north of Italy

Foschi

Nardini

Banzola

et al. 2016

Journal of Medical Microbiology

View full text Add to dashboard Cite

The aim of this study was to assess Chlamydia trachomatis (CT) infection prevalence and serovar distribution in a high-density urban area in the north of Italy, by comparing different groups of subjects divided on the basis of the type of care provider they referred to (STI Clinic, gynaecologists or general practitioners). From January 2011 to May 2014, all the specimens submitted to the Microbiology Laboratory of St Orsola Hospital in Bologna for CT detection were tested by PCR assay. For positive specimens, molecular genotyping based on RFLP analysis was performed. Total prevalence of CT infection was 8.1 %, with significant differences between subgroups (P<0.01) but stable during the study period. The STI Clinic was mainly responsible for CT diagnosis, whereas the lowest infection prevalence was detected in gynaecological clinics, despite the high number of tests performed. Extra-genital samples were almost exclusively collected from males at the STI Clinic. Interestingly, 13.3 % of patients providing extra-genital specimens were positive for CT on rectal and/or pharyngeal swabs, and 4.4 % of cases would have been missed if extra-genital sites had not been tested. The most common serovar was E, and serovar distribution was influenced by gender (P<0.01), age (P<0.01), care provider (P=0.01) and anatomical site (P<0.01). The L2 serovar was detected only in extragenital samples from males at the STI Clinic. Knowledge about care providers' contributions in CT testing and diagnosis is essential for infection control. CT typing is crucial for appropriate management of specific infections, such as lymphogranuloma venereum in extra-genital samples of high-risk populations.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chlamydia trachomatis infection prevalence and serovar distribution in a high-density urban area in the north of Italy

Foschi

Nardini

Banzola

et al. 2016

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…The availability of the whole genome sequence for C. trachomatis has led to the development of several new genotyping systems, such as multilocus sequence typing (MLST) and multilocus variable number tandem repeat analyses (MLVA), with a higher discriminating capacity [10][11][12]. However, these approaches have been used within the framework of the current ompA-based C. trachomatis classification system, as there is not yet a standardized nomenclature or classification system based on these novel typing methods [8].…”

Section: Introductionmentioning

confidence: 99%

Genotyping and prevalence of Chlamydia trachomatis infection among women in Belém, Pará, northern Brazil

Brasiliense

Borges

Ferreira

2016

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Genital Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases in women, and undetected cases of the disease are highly associated with long-term complications. Despite the high prevalence of infections in Brazil, very little is known about the distribution of C. trachomatis genovars. In this study, we determined the prevalence and genotypes of C. trachomatis in women treated at a public hospital in the Brazilian city of Belém, the capital of the state of Pará. Methodology: A total of 154 women were tested for chlamydial infection by PCR using specific primers for the C. trachomatis cryptic plasmid. Genotyping of positive samples was performed by sequencing the ompA gene and conducting further phylogenetic analysis. Results: Out of the 154 samples, 17 were found to be positive using C. trachomatis cryptic plasmid PCR. The overall prevalence of C. trachomatis infection was 11%, with the highest prevalence observed in women between 16 and 20 years of age. Five genotypes were found to be associated with endocervical infection. Genotype F was most frequently found (37.5%), followed by genotypes J (25%), E (25%), I (6.25%), and D (6.25%). Conclusions: This study emphasizes the relevance of C. trachomatis infection in the young female population of the Brazilian Amazon region. It also demonstrates the diversity of genotypes involved in genital infection in this population.

show abstract

“…It has been applied to specimens from heterosexual populations in a number of studies and has shown up to a 5-fold-higher resolution than ompA genotyping, revealing a large diversity of clinical strains missed by ompA genovar typing (7,11,16,18). In a modified version, high resolution was retained but sensitivity was im-proved by targeting smaller parts of the polymorphic genetic regions (4).…”

mentioning

confidence: 99%

Chlamydia trachomatis Strains Show Specific Clustering for Men Who Have Sex with Men Compared to Heterosexual Populations in Sweden, the Netherlands, and the United States

et al. 2012

Self Cite

View full text Add to dashboard Cite

f High-resolution genotyping of Chlamydia trachomatis improves the characterization of strains infecting different patient groups and sexual networks. In this study, multilocus sequence typing (MLST) and ompA sequence determination were used for an analysis of C. trachomatis strains from 203 men who have sex with men (MSM) from Sweden, the Netherlands, and the United States. The results obtained were compared with data from 153 heterosexual women from Sweden and the Netherlands. The overlap in MLST/ompA profiles between MSM from Sweden and the Netherlands was 68%, while the overlap between heterosexual populations from these countries was only 18%. The distribution of genotypes in MSM from the United States was less similar to that in MSM from the European countries, with 45% and 46% overlaps for MSM in Sweden and the Netherlands, respectively. Minimum-spanning-tree analysis of MLST/ompA sequence types identified two large clusters that contained almost exclusively samples from MSM and comprised 74% of all MSM samples. Three other clusters were predominated by samples from women but also contained MSM specimens. Of 19 detected variants of the MLST target CT144, three variants were highly associated with MSM. Our study supports the hypotheses of both tissue tropism as well as epidemiological network structures as explanations for the linkage between specific genetic variants and sexual orientation.

show abstract

Evaluation of High-Resolution Typing Methods for Chlamydia trachomatis in Samples from Heterosexual Couples

Cited by 76 publications

References 19 publications

Chlamydia trachomatis infection prevalence and serovar distribution in a high-density urban area in the north of Italy

Chlamydia trachomatis infection prevalence and serovar distribution in a high-density urban area in the north of Italy

Genotyping and prevalence of Chlamydia trachomatis infection among women in Belém, Pará, northern Brazil

Chlamydia trachomatis Strains Show Specific Clustering for Men Who Have Sex with Men Compared to Heterosexual Populations in Sweden, the Netherlands, and the United States

Contact Info

Product

Resources

About