Evaluation of highly sensitive indigenous milk ELISA kit with fecal culture, milk culture and fecal-PCR for the diagnosis of bovine Johne's disease (BJD) in India

Singh, Shoor Vir; Singh, A. V.; Singh, Rajbir; Sandhu, K. S.; Singh, Pravin Kumar; Sohal, J.S.; Gupta, Vinod K.; Vihan, V. S.

doi:10.1016/j.cimid.2006.12.002

Cited by 33 publications

(23 citation statements)

References 20 publications

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“…Kit had high sensitivity and specificity as compared to commercial ELISA kits (Singh et al, 2009b). Indigenous ELISA was optimally correlated with culture and was good for estimating the sero-prevalence (Singh et al, 2007c). Indigenous g-ELISA was superior than AGPT for the diagnosis of MAP infection in cattle (Pahangchopi et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

Chaubey¹,

Singh²,

Gupta³

et al. 2015

Asian J. of Animal and Veterinary Advances

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Cross reactivity of three antigens of Mycobacterium avium subspecies paratuberculosis with sera of sheep endemic for Johne's disease was evaluated. Out of 40 sheep tested by fecal microscopy, 72.5% were shedding MAP. Using protoplasmic antigens (PPA) from three MAP strains isolated from different livestock species and geographical regions, 90, 77.5 and 2.5% sheep were positive in goat (Indigenous g-ELISA) and cattle (b-ELISA) based ELISA kits and ELISA kit for small ruminant (sr-ELISA), respectively. Only 2.5 and 10% sheep were positive and negative in all the four tests. Native species specific (goat origin novel 'Indian Bison Type' MAP) semi-purified whole cell PPA based ELISA (Indigenous g-ELISA) was superior in reacting with sera of native sheep than the commercial PPA of bovine origin (Allied Monitor Inc., USA) and commercial ELISA kit for small ruminants (ID Vet, France). Lower cross reactivity of antigens originated from US and France emphasized the need to develop tests based on local strain of MAP than strains from different livestock species and geographical regions. This is an important finding against the use of 'Global kits' without validating in local conditions. Study showed that kits developed from local strains of MAP were not only superior but also cost effective and will significantly contribute in programs for the control of JD in native sheep population.

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Section: Discussionmentioning

confidence: 99%

Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

Chaubey¹,

Singh²,

Gupta³

et al. 2015

Asian J. of Animal and Veterinary Advances

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show abstract

“…Zoonotic potential of MAP has been generated renewed concerns, since bacilli has been frequently reported from the foods of animal origin, specially milk and milk products made from pasteurized milk (Singh et al, 2007). Human exposure to MAP by consumption of pasteurized milk and milk products has been regarded as a major risk factor for the development of number of chronic, incurable and auto-immune type disorders (Crohn's disease, type-1 diabetes, Sarcoidosis, Multiple sclerosis and Hashimoto's thyroiditis among others) (Sechi & Dow, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The test was performed as per the method of Singh et al (2007). Briefly, Milk based indigenous "p-ELISA" kit was used as standard test for screening of animals for MAP infection and parallel test was used as for the for comparative studies.…”

Section: Indigenous Milk Elisa Testmentioning

confidence: 99%

Evalution of “indirect fluorescent antibody test‟ as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock

Singh¹,

Stephen²,

Singh³

et al. 2016

JEBAS

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In the present study, Indirect Fluorescent antibody test (iFAT) has been used as the "screening test" for the detection of MAP bacilli in the milk samples of lactating domestic livestock. A total of 372 milk samples from lactating animals were screened by iFAT and results were compared with microscopy of milk samples and milk ELISA test. Comparative analysis of the results of three tests showed that iFAT had fairly good sensitivity (84.7%) and specificity (90.4%), with respect to ZN staining (microscopy) with kappa value of 0.735 and "good" strength of agreement. Similar comparison with milk ELISA test revealed, sensitivity (73.3%) and specificity of (72.6%) with kappa value of 0.443 and strength of agreement was moderate. Lower or higher difference in sensitivity and specificity of iFAT with respect to ZN staining and milk ELISA may be due to the difference in detection "target" of the test i.e., antigen or antibody. It was concluded that iFAT was a reliable and sensitive diagnostic test for the detection of MAP in milk and can also be used for the "mass screening" of the milk samples.

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“…Country has huge population (>500 million) of domestic livestock, endemically infected with MAP Rawat et al, 2014). We have reported high to very high bio-incidence of MAP in the raw milk (individual animals and pooled milk) of domestic livestock (Singh et al, 2007;Shankar et al, 2010;Singh et al, 2014). At 155.5 million tonnes (MT) of milk produced in 2016-17, India is leading milk producer in the world (Annual Report, DADF, 2016 and approximately 40.0% of milk is pasteurized, predominantly by state cooperatives, multi-national companies, or government dairy plants (India in business, Investment and technology promotion and energy security, Delhi: Ministry of External Affairs, Government of India, 2008) for the preparation of various milk products.…”

Section: Introductionmentioning

confidence: 95%

BIO-INCIDENCE OF Mycobacterium avium SUBSPECIES paratuberculosis IN THE PASTEURIZED LIQUID MILK, FLAVOURED MILK AND MILK POWDER COMMERCIALLY SOLD BY LEADING MARKET BRANDS IN INDIA

Singh¹,

Singh²,

Gupt³

et al. 2018

JEBAS

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Evaluation of highly sensitive indigenous milk ELISA kit with fecal culture, milk culture and fecal-PCR for the diagnosis of bovine Johne's disease (BJD) in India

Cited by 33 publications

References 20 publications

Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

Evalution of “indirect fluorescent antibody test‟ as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock

BIO-INCIDENCE OF Mycobacterium avium SUBSPECIES paratuberculosis IN THE PASTEURIZED LIQUID MILK, FLAVOURED MILK AND MILK POWDER COMMERCIALLY SOLD BY LEADING MARKET BRANDS IN INDIA

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