Evaluation of human chaperonin 10 and high-sensitivity C-reactive protein levels of infertile women who underwent ovulation induction and intra-uterine insemination

Abstract

The implantation of embryo is one of the crucial steps of a successful pregnancy. The foetus should be protected from maternal immune system, for the appropriate implantation and modification in maternal immunity is crucial. We investigated high-sensitivity C-reactive protein (hs CRP), which is an indicator of low-grade inflammation and Cp10 that has immunosuppressant and growth-promoting capabilities at embryo levels in ovulation induction and intra-uterine insemination (IUI)applied in infertile women. The ovulation induction was maintained by clomiphene citrate or gonadotropins for 42 infertile patients. After successful ovulation induction, IUI was carried out. The blood samples were taken 2 and 8 days after IUI to evaluate Cp10 and hs CRP levels. The pregnant and non-pregnant groups' results were analyzed. The Cp10 levels 8 days after IUI were higher in pregnant group, whereas there was no difference for the 2 days after levels between pregnant and non-pregnant group. The hs CRP levels were similar for both 2nd and 8th days when we compared pregnant and non-pregnant groups. The Cp10 levels increased from day 2 to day 8 in pregnant group. In contrast, the Cp10 levels decreased in non-pregnant group. The change in hs CRP levels from day 2 to day 8 was not significant in pregnant and non-pregnant groups. The Cp10 levels were higher in early phases of fertilisation and elevated through the preceding days of conception in pregnant patients, while it decreased in non-pregnant patients with failed cycles.

High-sensitivity C-reactive protein levels and pregnancy outcomes in women with unexplained infertility after ovarian stimulation with intrauterine insemination in a multicenter trial

“…However, the group that achieved pregnancy was significantly younger on average (25.5 ± 5.4 vs. 29.2 ± 5.4 years, P =.012), which was not adjusted for in the analysis ( 21 ). Another similar study compared the hs-CRP levels 2 and 8 days after IUI and found no difference between pregnant and non-pregnant patients, but this study was limited by its small sample size (n = 42), and only 8 patients became pregnant ( 22 ).…”

Serum CRP level in day of ovum pickup and embryo transfer as a predictor for success in patients undergoing IVF/ ICSI

“…these changes may affect the success rate of IVF/ICSI, implantation, and pregnancy, also adminstration of human chorionic gondotropin (HCG) regardless of the response rate of the ovaries, causes activation of endothelial cells and neutrophils. [7][8][9] The level of CRP increases in ovulation stimulation cycle with standard long protocol. Wunder et al in 2005 showed that leptin and CRP in ovulation stimulation cycle increase until the day of puncture, 9 Orvieto et al showed the same results.…”