Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study

Abstract

ObjectiveWe investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development.MethodsA total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination.ResultsNo significant differences were observed between the two groups regarding clinical outcomes (p>0.05). ICSI-derived embryos showed significantly faster morphokinetics than those derived from conventional IVF, from the time to pronuclear fading to the time to 6 cells (p<0.05). However, no significant differences were found from the time to 7 cells to the time to expanded blastocyst (p>0.05). There were no differences in abnormal cleavage events between the two groups (p>0.05); they showed the same rates of direct cleavage from 1 to 3 cells, 2 multinucleated cells, 2 uneven cells, and reverse cleavage.ConclusionThe morphokinetics of embryo development was found to vary between IVF- and ICSI-fertilized oocytes, at least until the 6-cell stage. However, these differences did not affect the clinical outcomes of the embryo. Additionally, no significant differences in abnormal cleavage events were found according to the fertilization method.

Time-lapse imaging derived morphokinetic variables reveal association with implantation and live birth following in vitro fertilization: A retrospective study using data from transferred human embryos

“…The IVF embryos exhibited a delay of 0.28 hours in tPNf compared to ICSI embryos. This average time lag is lower than in previously published studies [ 36 , 37 ]. The analysis does not distinguish between IVF and ICSI embryos.…”

IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

“…It also runs morphological, dynamic and morphokinetic parameters. These factors can be used to estimate embryo viability and implantation potential (Coticchio et al, ; Kim et al, ; Wu et al, ).…”

In assisted reproduction by IVF or ICSI, the rate at which embryos develop to the blastocyst stage is influenced by the fertilization method used: a split IVF/ICSI study

“…Lemmen et al [ 15 ] using a time-lapse monitoring system supported this observation. The faster development of ICSI-derived embryos is reported to last until the 5-cell [ 20 ] or 6-cell [ 13 ] stage.…”

“…This theory, that IVF-derived embryos are fertilized by sperm superior to those fertilizing ICSI, has an advantage over any attempt to explain the slower rate of blastocyst formation by ICSI on proposed delays related to the ICSI process. The superior-sperm theory explains why ICSI-derived embryos start their development faster than IVF-derived embryos and only become slower at the 5–6-cell stage when the male genes fully participate in the genome [ 4 , 13 , 15 , 20 ].…”