Evaluation of human recombinant bone morphogenetic protein-2-loaded tricalcium phosphate implants in rabbits’ bone defects

Laffargue, P.; Hildebrand, Hartmut F.; Rtaimate, M.; Frayssinet, Patrick; Amoureux, Jean‐Paul; Marchandise, X.

doi:10.1016/s8756-3282(99)00134-9

Cited by 62 publications

(40 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rabbits are also advantageous compared to rats because they exhibit a predictable cessation of longitudinal bone growth after five to six months (Kaweblum et al, 1994) and should not require special processing of images to account for growth as seen with rats (Waarsing et al, 2004a). In vivo micro-CT scanning of rabbits will also complement studies involving bone graft incorporation, implant bone interfaces, and skeletal assessment which commonly use the rabbit femur model (Laffargue et al, 1999;Orr et al, 2001;Ohsawa et al, 2004;Voor et al, 2004;Bourne and van der Meulen, 2004;Saha and Wehrli, 2004;Pihlajamaki et al, 2006a;Pihlajamaki et al, 2006b;Dallari et al, 2006;Fellah et al, 2006;Takahashi et al, 2006;Wang et al, 2006;van der Meulen et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

In vivo micro-CT scanning of a rabbit distal femur: Repeatability and reproducibility

Voor

Yang

Burden

et al. 2008

Journal of Biomechanics

View full text Add to dashboard Cite

Before in vivo micro-CT scanning can be used to investigate femoral trabecular microarchitecture over time in rabbits, its repeatability and reproducibility must be demonstrated. To accomplish this, both distal femurs of two six-month-old New Zealand white rabbits were scanned five times each in one day under different conditions (repeatability). Scanning was done at 28 μm isotropic voxel size to produce five image stacks of each femur. Three operators then followed a standard image processing protocol (reproducibility) to isolate two separate cubes from each anterior femoral condyle [total n = (8 cube sites)(5 scans)(3 operators) = 120]. Bone volume fraction (BV/TV) of the eight different cube sites (sample) ranged from 0.408 to 0.501 (mean: 0.453); trabecular thickness (Tb.Th) ranged from 158.1 μm to 185.5 μm (mean: 168.6 μm); and trabecular separation (Tb.Sp) ranged from 179.4 μm to 233.1 μm (mean: 204.7 μm). Using ANOVA and the variance component method, the total process variation was ± 14.1% of the mean BV/TV of 0.453. The sample variation was ± 13.9% (p<0.001), the repeatability was ± 2.1% (p<0.001), and the reproducibility was ± 0.1% (p>0.05). Results were similar for Tb.Th and Tb.Sp. Though the contribution due to repeatability was statistically significant for each of the three indices, the natural sample differences were far greater than differences caused by repeated scanning under different conditions or by different operators processing the images. These findings suggest that in vivo micro-CT scanning of rabbit distal femurs was repeatable and reproducible and can be used with confidence to measure differences in trabecular bone microarchitecture at a single location in a longitudinal study design.

show abstract

Section: Introductionmentioning

confidence: 99%

In vivo micro-CT scanning of a rabbit distal femur: Repeatability and reproducibility

Voor

Yang

Burden

et al. 2008

Journal of Biomechanics

View full text Add to dashboard Cite

show abstract

“…PLA and PGA are two well-known polymers of alphahydroxyl esters and copolymers of these monomers (PLGA) are good vehicles to carry osteoinductive factors (for review see Behravesh et al, 1999;Saito et al, 2001a, as cited in Lee et al, 2007). Calcium phosphate cement (CPC), bioactive glasses, HA and beta-TCP are non-polymeric, inorganic materials that have also been processed to deliver growth factors such as TGF, beta1 and BMPs (for review see Hedberg et al, 2005;Laffargue et al, 1999;Ripamonti et al, 1992). The design of a delivery system, in the form of three-dimensional matrices, injectable gels, and micro/nano particulates determines release kinetics and stability of growth factors in constructs (Lee et al, 2007).…”

Section: Growth Factor Delivery In Bone Tissue Engineeringmentioning

confidence: 99%

Mesenchymal Stem Cell-Based Bone Engineering for Bone Regeneration

Eslaminejad¹,

Faghihi²

2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

View full text Add to dashboard Cite

“…17) Various materials as a BMP carrier have already been examined, such as -TCP, 18) hydroxyapatite, 19) biphasic calcium phosphate, 20) collagen, 21) polylactic acid-polyglycolic acid copolymer, 22) and titanium. 23) Several studies 18,[24][25][26] indicate that -TCP can be used as a slow-release delivery system for BMP and potentiates the activity of BMP. In this study, we examined cages filled with -TCP and rhBMP-2 to improve the success rate for bony fusion.…”

Section: Introductionmentioning

confidence: 99%

β-Tricalcium Phosphate Combined with Recombinant Human Bone Morphogenetic Protein-2: A Substitute for Autograft, Used for Packing Interbody Fusion Cages in the Canine Lumbar Spine

et al. 2004

View full text Add to dashboard Cite

The use of -tricalcium phosphate (-TCP) as osteoconductive and bone morphogenetic protein-2 (BMP-2) as osteoinductive bone graft substitutes has recently gained considerable research interest in spine surgery. However, whether the combination can be extrapolated to a successful interbody spinal arthrodesis remains uncertain. In this study, -TCP combined with recombinant human BMP-2 was examined in the canine lumbar spine model as a substitute for autograft used for packing interbody fusion cages. The discectomy and interbody cage fusion were performed at three disc spaces in 8 dogs. The examination of microradiographs and histological sections of the lumbar spine at 16 weeks postsurgery revealed three fusions in autograft group (A), three -TCP group (B), and five in -TCP-BMP-2 (C). The mean percentage of trabecular bone area in the cages was 51.9% in group A, 48.8% in group B and 65.6% in group C. Statistical analyses of the results could not give significance. The young animal model used in this study might suppress difference between the groups. As mentioned, however, actual data of mean percentage of trabecular bone formation and of mechanical stiffness were largest in the cages filled with -TCP and BMP-2. In clinical settings, we expect that a cage filled with -TCP and BMP gives beneficial effects especially for patients with poor fusion statuses.

show abstract

Evaluation of human recombinant bone morphogenetic protein-2-loaded tricalcium phosphate implants in rabbits’ bone defects

Cited by 62 publications

References 8 publications

In vivo micro-CT scanning of a rabbit distal femur: Repeatability and reproducibility

In vivo micro-CT scanning of a rabbit distal femur: Repeatability and reproducibility

Mesenchymal Stem Cell-Based Bone Engineering for Bone Regeneration

β-Tricalcium Phosphate Combined with Recombinant Human Bone Morphogenetic Protein-2: A Substitute for Autograft, Used for Packing Interbody Fusion Cages in the Canine Lumbar Spine

Contact Info

Product

Resources

About

Evaluation of human recombinant bone morphogenetic protein-2-loaded tricalcium phosphate implants in rabbits’ bone defects

Cited by 62 publications

References 8 publications

In vivo micro-CT scanning of a rabbit distal femur: Repeatability and reproducibility

In vivo micro-CT scanning of a rabbit distal femur: Repeatability and reproducibility

Mesenchymal Stem Cell-Based Bone Engineering for Bone Regeneration

&beta;-Tricalcium Phosphate Combined with Recombinant Human Bone Morphogenetic Protein-2: A Substitute for Autograft, Used for Packing Interbody Fusion Cages in the Canine Lumbar Spine

Contact Info

Product

Resources

About

β-Tricalcium Phosphate Combined with Recombinant Human Bone Morphogenetic Protein-2: A Substitute for Autograft, Used for Packing Interbody Fusion Cages in the Canine Lumbar Spine