Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein

Chandra, Sangita; Chatterjee, Priyanka; Dey, Protapaditya; Bhattacharya, Sanjib

doi:10.1016/s2221-1691(12)60154-3

Cited by 332 publications

(186 citation statements)

References 5 publications

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“…After cooling, the absorbance was measured at 660 nm. Diclofenac sodium was used as reference drug [22].…”

Section: Albumin Denaturation Assaymentioning

confidence: 99%

Evaluation of Antioxidant and Anti-Inflammatory Properties of Aqueous Extract of Wild Mushrooms Collected From Himachal Pradesh

Bains¹,

Tripathi²

2017

Asian J Pharm Clin Res

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Objective: The objective of this study is to elucidate phytochemical contents, antioxidant, and anti-inflammatory activity of wild mushrooms collected from Himachal Pradesh.Methods: Quantitative analysis of phytochemical constituents was carried out using standard methods while 1,1 diphenylpicrylhydrazyl nitric oxide, hydrogen peroxide-free radical scavenging assay were used to evaluate the antioxidant properties of selected wild mushrooms. Anti-inflammatory capacity of samples was evaluated by human red blood cell membrane stabilization and albumin denaturation inhibition method. Results:The results obtained revealed that Pleurotus floridanus showed higher total phenol, flavonoid carotenoid, and ascorbic acid contents of 61.13±2.3 mg/g, 15.2±1.13 mg/g, 12.42±0.42 µg/g, 17.36±0.40 µg/g, and 14.55±0.58 mg/g. All the species showed antioxidant potential, but P. floridanus proved to be more active while Macrocybe sp. proved to be least one. Conclusion:The mushroom species analyzed have been showed to be good source of phytoconstituents, antioxidants, and also possess antiinflammatory properties, thus can be used in the management of oxidative stress-induced disease.

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“…After cooling, the absorbance was measured at 660 nm. Diclofenac sodium was used as reference drug [22].…”

Section: Albumin Denaturation Assaymentioning

confidence: 99%

Evaluation of Antioxidant and Anti-Inflammatory Properties of Aqueous Extract of Wild Mushrooms Collected From Himachal Pradesh

Bains¹,

Tripathi²

2017

Asian J Pharm Clin Res

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“…The Inhibition of albumin denaturation was assessed according to the modified method of (Chandra et al, 2012). Briefly, one mL of 1% bovine serum albumin prepared in PBS (phosphate-buffered saline, pH 6.4) was added to one mL of varying concentrations of plant filtrate.…”

Section: Inhibition Of Albumin Denaturationmentioning

confidence: 99%

In vitro Antioxidant and anti-inflammatory properties of selected Moroccan medicinal plants

Bouhlali¹,

Sellam²,

Bammou³

et al. 2016

J App Pharm Sci

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The aim of the present study was to examine the antioxidant and anti-inflammatory activities of three Moroccan ethno medicinally important plants. The antioxidant activity was performed using β-Carotene bleaching assay, DPPH, ABTS and Ferric reducing assays. In vitro anti-inflammatory assays was also studied through the evaluation of membrane stabilization potential and inhibition of protein denaturation. Results revealed that Lawsonia inermis L leaves extract had significantly higher phenolics-content (5.23 g GAE/100g DW) and exhibited the highest antioxidant activity based on DPPH (IC50 = 18.26 µg/mL). The highest flavonoids content was found in Rosa damascena. L flower (3.97 RE/100g DW) which shows the highest antioxidant activity based on ABTS (30.39 mmol TE/100g DW), FRAP (38.54 mmol TE/100gDW) and β-Carotene bleaching assay (IC50 = 56.33 µg/mL). Concerning the anti-inflammatory potency, the highest membrane stabilization effect was found in Rosa damascena. L flower (IC50 = 125.02 µg/mL) and the highest anti-denaturation of protein effect was found in Lawsonia inermis L leaves extract (IC50 = 103.21 µg/mL). Consequently, to conclude flavonoids and related phenolics present in studied plant materials may possibly be responsible for the important antiinflammatory and antioxidant activities.

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“…While, the viscosities of the cream formulation of all concentrations were always found less than that of control. The effect of concentration of test agent on the viscosity behaviour of denatured protein dispersion requires further studies 27,28 . It was also noted that the reducing power anti-oxidant activity correlated strongly with anti-inflammatory activity as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Untitled

2017

IJPSR

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ABSTRACT:Biologically active compounds identified as flavonoids, glycosides, alkaloids, saponins, terpenoids and polyphenolics from plant extracts possess health promoting properties. The use of natural antioxidants in the treatment of various skin diseases has gained utmost importance in recent years. Therefore, present study was designed and conducted with the aim to evaluate the anti-inflammatory activity of cream formulation containing fruit extracts of Musa acuminata, Psidium guajava and Pyrus communis using in vitro model. Fruit of M. acuminata, P. guajava and P. communis were extracted by cold percolation, comprising of ethanol, methanol and distilled water. The cream formulation and the reference drug (Diclofenac sodium) of varying concentrations were incubated with fresh egg albumin under specified experimental conditions and subjected to determination of absorbance and viscosity to assess the in vitro antiinflammatory activity. Linear regression analysis was used to calculate IC 50 value. The results revealed that cream formulation and diclofenac sodium inhibited protein denaturation in a dose-dependent manner. The IC 50 value of cream formulation 1254.13±16.91µg/ml and diclofenac sodium was recorded as 845.82±16.94µg/ml at correlation coefficient value (r) of 0.900 and 0.983 respectively. The study gives an idea that the fruits extract can be used as a lead compound for designing a potent topical anti-inflammatory drug.

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Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein

Cited by 332 publications

References 5 publications

Evaluation of Antioxidant and Anti-Inflammatory Properties of Aqueous Extract of Wild Mushrooms Collected From Himachal Pradesh

Evaluation of Antioxidant and Anti-Inflammatory Properties of Aqueous Extract of Wild Mushrooms Collected From Himachal Pradesh

In vitro Antioxidant and anti-inflammatory properties of selected Moroccan medicinal plants

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