Evaluation of indigenous Trichoderma isolates from Manipur as biocontrol agent against Pythium aphanidermatum on common beans

Kamala, Th.; Indira, S.

doi:10.1007/s13205-011-0027-3

Cited by 37 publications

(24 citation statements)

References 30 publications

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“…(B) its preparation is easy and inexpensive; (C) the possibility of interference of BCP is minimal under the environmental conditions used ordinarily in the chitinase assays; (E) as colloidal chitin (CC)-BCP is not toxic for microorganisms, it can be added simultaneously as a carbon source and as a chitinase inducer in the culture media. This fact allows a simpler, fast and accurate one-step process for the selection of chitinolytic microorganisms and was therefore also followed by Kamala and IndiraDevi (Kamala & IndiraDevi 2011; Kamala & IndiraDevi 2012) to evaluate the chitinolytic properties of Trichoderma isolates from Manipur (North-East India) against Pythium aphanidermatum , Fusarium oxysporum and Rhizoctonia solani .…”

Section: Resultsmentioning

confidence: 99%

“…More sensitive techniques require more expensive substrates, which are suitable to study specificity of chitinases more than to select chitinolytic strains (O’Brien & Collwell 1987; McCreath & Gooday 1992; Fra¨ndberg & Schnu¨rer 1994; Barboza Corona et al 1999). Agrawal and Kotasthane (Agrawal & Kotasthane 2009) proposed a sensitive, easy, reproducible and economic option to determine chitinases (available via Dialog, http://www.isth.info/methods/method.php?method_id=11 ) which was also followed by Kamala and IndiraDevi (Kamala & IndiraDevi 2011; Kamala & IndiraDevi 2012) to evaluate the chitinolytic properties of Trichoderma isolates from Manipur (North-East India) against Pythium aphanidermatum , Fusarium oxysporum and Rhizoctonia solani .…”

Section: Introductionmentioning

confidence: 99%

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Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India

2012

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Chitin is the second most abundant polymer in nature after cellulose and plays a major role in fungal cell walls. As a producer of variety of chitinase enzymes Trichoderma has become an important means of biological control of fungal diseases. A simple and sensitive method based on the use of basal medium with colloidal chitin as sole carbon source supplemented with Bromo cresol purple (pH indicator dye) is proposed to evaluate large populations of Trichoderma for chitinase activity. The soluble substrate with pH indicator dye (Bromo cresol purple, BCP) for the assay of chitinase activity on solid media is sensitive, easy, reproducible semi-quantitative enzyme diffusion plate assay and economic option to determine chitinases. Colloidal chitin derived from Rhizoctonia cell wall and commercial chitin included as a carbon source in broth also allowed selection and comparison of chitinolytic and exochitinase activity in Trichoderma spectrophotometrically. Released N-acetyl-β--D-glucosamine (NAGA) ranged from 37.67 to 174.33 mg/ml and 37.67 to 327.67 mg/ml and p-nitrophenol (pNP) ranged from 0.17 to 35.78 X 10-3 U/ml and 0.62 to 32.6 X 10-3 U/ml) respectively with Rhizoctonia cell wall and commercial chitin derived colloidal chitin supplemented broth.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India

2012

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“…solani (Figure 3b ). It could be due to the production of hydrolytic enzymes by certain fungal isolates, or the production of antibiotics by the antagonists (Kamala and Indira, 2011 ). The various types of interactions we observed are dependent on the specific fungal species combination, because of differences in compounds of the volatile mixture, substrates, or abilities to detoxify the volatile metabolites such as enzymatic activity (Kai et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity and Characterization of Endophytic Fungi Isolated From the Tropical Mangrove Species, Rhizophora mucronata, and Identification of Potential Antagonists Against the Soil-Borne Fungus, Fusarium solani

et al. 2018

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Rhizophora mucronata is an important ecosystem entity of the Malaysian mangrove forest. Since the species grows in a harsh environment, any organism that is isolated from this species would be of huge interest due to its potential in having novel bioactive compounds. In the present work, we isolated, identified and characterized, a total of 78 fungal isolates harboring inside the leaf tissues of R. mucronata. Molecular identification using the nuclear ribosomal DNA internal transcribe spacer (ITS) sequences returned with high similarity matches to known sequences in the GenBank. Maximum likelihood analysis revealed the phylogenetic relationship of all isolates from this study. Most of the dominating fungal endophytes were from the genera Pestalotiopsis, followed by Alternaria and Cladosporium. Six isolates representing the genera Alternaria, Fusarium, Nigrospora, Pestalotiopsis, Phoma, and Xylaria, were further screened for their antagonism activities. Dual culture test assay revealed their inhibition percentages against the phytopathogenic fungus Fusarium solani between 45–66%, and 0.8–23% when using non-volatile test assay. Of the six isolates, only Fusarium lateritium and Xylaria sp. showed antibacterial activities against the pathogenic bacteria, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, with the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) ranging from 0.5 to 2 mg/mL. The DPPH radical scavenging assay recorded a high level of antioxidant activity in Xylaria sp., 3-fold above that of F. lateritium. We demonstrate for the first time, two members belonging to the endophytic fungal community in the tropical mangrove species that have potential use as antagonists and antibacterial agents for future biotechnological applications.

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“…Trichoderma genus has been shown effective as biocontrol agent against a range of economically important aerial and soil borne fungal plant pathogens [8]. Although mutations of Tricho derma spp.…”

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confidence: 99%

Novel mutations in β-tubulin gene in Trichoderma harzianum mutants resistant to methyl benzimidazol-2-YL carbamate

Zhang

Liang

2013

Appl Biochem Microbiol

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Twelve low resistant (LR) mutants of Trichoderma harzianum with the capability of grow fast at 0.8 μg/mL methyl benzimidazol 2 yl carbamate (MBC) were obtained using UV mutagenesis. MR and HR mutants which could grow fast at 10 and 100 μg/mL MBC, respectively, were isolated by step up selection protocols in which UV treated mutants were induced and mycelial sector screening was made in plates with growth medium. Subsequently, β tubulin genes of 14 mutants were cloned to describe the molecular lesion likely to be responsible for MBC resistance. Comparison of the β tubulin sequences of the mutant and sen sitive strains of T. harzianum revealed 2 new MBC binding sites differed from those in other plant pathogens. A single mutation at amino acid 168, having Phe (TTC) instead of Ser (TCC), was demonstrated for the HR mutant; a double mutation in amino acid 13 resulting in the substitution of Gly (GGC) by Val (GTG) was observed in β tubulin gene of MR mutant. On the other hand, no substitutions were identified in the β tubu lin gene and its 5' flanking regions in 12 LR mutants of T. harzianum. 165Ala to Val A. nidulans [ 23] 167 Phe to Tyr Cochliobolus heterostrophus [24], Neurospora crassa [25], Penicillium expansum [26], Saccharomyces cerevisiae [27] 198 Glu to Ala Botrytis cinera [28], M. fructicola [19], Penicillium aurantiogriseum [29], P. expansum [26], Tapesia yallundae [30], Venturia inaequalis [29] Glu to Asp A. nidulans [18] Glu to Gln A. nidulans [18], T. yallundae [30] Glu to Gly N. crassa [31], T. yallundae [30] Glu to Lys A. nidulans [18], Colletotrichum.gloeosporioides [32], M. fructicola [19], P. aurantiogri seum [29], P. expansum [26], Sclerotinia homoeocarpa [29], T. yallundae [30], V. inaequalis [29] Glu to Val P. expansum [26] 200 Glu to Lys B. cinera [33] Phe to Tyr P. aurantiogriseum [29], T. yallundae [30], V. inaequalis [29] 240 Leu to Phe M. laxa [34], T. yallundae [30] 241 Arg to His S. cerevisiae [35]

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Evaluation of indigenous Trichoderma isolates from Manipur as biocontrol agent against Pythium aphanidermatum on common beans

Cited by 37 publications

References 30 publications

Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India

Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India

Diversity and Characterization of Endophytic Fungi Isolated From the Tropical Mangrove Species, Rhizophora mucronata, and Identification of Potential Antagonists Against the Soil-Borne Fungus, Fusarium solani

Novel mutations in β-tubulin gene in Trichoderma harzianum mutants resistant to methyl benzimidazol-2-YL carbamate

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