Evaluation of ionic liquid stationary phases for one dimensional gas chromatography–mass spectrometry and comprehensive two dimensional gas chromatographic analyses of fatty acids in marine biota

Gu, Qun; David, Frank; Lynen, Frédéric; Vanormelingen, Pieter; Vyverman, Wim; Rumpel, Klaus; Xu, Guowang; Sandra, Pat

doi:10.1016/j.chroma.2011.03.011

Cited by 73 publications

(61 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the last stage, the upper layer was moved to a vial for analysis. The operating conditions for FAME analysis were carried out according to Gu et al, (2011) with a few modifications. Separations were performed on a HP-5MS (30m×0.25mm×0.25µm, Agilent) capillary column.…”

Section: Determination Of the Fatty Acid Composition By Gc-fidmentioning

confidence: 99%

Cold-pressed pumpkin seed (<em>Cucurbita pepo</em> L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

et al. 2018

View full text Add to dashboard Cite

SUMMARY:There is a growing interest in cold-pressed oils because they present high contents of bioactive compounds. These oils have the characteristic properties of seeds and are specific products of their regions. The aim of this study was to determine the compositions and contents of fatty acids, phytosterols, squalene, tocols, phenolic acids, carotenoids and phenolic bioactives, and the free radical scavenging as well as antioxidant activities of coldpressed pumpkin (Cucurbita pepo L.) seed oils. Oil samples from raw pumpkin seeds cultivated in four different central Anatolia regions of Turkey were prepared using a laboratory screw-pressing machine. The results indicate that coldpressed pumpkin seed oils (PSO) have excellent quality with high contents of polyunsaturated fatty acids (%∑PUFAs) (53.60 ±0.06−53.73 ±0.05), total phytosterols (782.1±9.7-805.2 ±11.3 mg/100 g oil), squalene (591.3±10.6-632.5±11.4 mg/100 g oil), tocols (97.79 ±0.76−94.29 ±0.34 mg/100 g oil), phenolic acids (22.73 ±0.41-23.98 ±0.46 mg/100 g oil), carotenoids (6.95 ±0.03-7.60 ±0.03 mg/100 g oil), total phenolics (3.96 ±0.13 -5.82±0.15 mg GAE/100 g), free radicalscavenging activity (5.70 ±0.13−7.35 ±0.15 mg GAE/100 g) and total antioxidant activity (26.67±0.97−38.89±1.41 mg GAE/100 g) values. Thus, this study demonstrates that the cold-pressed PSOs from the central Anatolia regions of Turkey are an excellent source of natural bioactive compounds, free of chemical contaminants and nutritious.KEYWORDS: Antioxidant activity; Bioactive compound; Cold-pressed oil; Cucurbita pepo L.; Phenolic acid; Tocol profile RESUMEN: Aceites de semillas de calabaza prensados en frío (Cucurbita pepo L.) de la región central de Anatolia en Turquía: Caracterización de fitosteroles, escualeno, tocoles, ácidos fenólicos, carotenoides y compuestos bioactivos de ácidos grasos. Existe un interés creciente en los aceites prensados en frío, ya que presentan altos contenidos de compuestos bioactivos. Estos aceites tienen las propiedades características de las semillas y son productos específicos de las regiones. El objetivo de este estudio fue determinar las composiciones y contenidos de ácidos grasos, fitoesteroles, escualeno, tocoles, ácidos fenólicos, carotenoides y bioactivos fenólicos, la actividad de eliminación de radicales libres, así como las actividades antioxidantes de aceites de semillas de calabaza prensados en frío (Cucurbita pepo L.). Las muestras de aceite de semillas de calabaza cultivadas en cuatro regiones diferentes de Anatolia central de Turquía se prepararon utilizando una máquina de laboratorio de prensado mediante tornillo. Los resultados indican que los aceites de semilla de calabaza prensados en frío (PSO) tienen una excelente calidad, con altos contenidos de ácidos grasos poliinsaturados (%ΣPUFA) (53,60±0,06-53,73±0,05), fitoesteroles totales (782,1±9,7-805,2±11,3 mg/100 g de aceite), escualeno (591,3 ± 10,6-632,5 ± 11,4 mg/100 g de aceite), tocols (97,79±0,76-94,29±0,34 mg/100 g de aceite), ácidos fenólicos (22,73 ± 0,41-23,98 ± 0.46 mg/10...

show abstract

Section: Determination Of the Fatty Acid Composition By Gc-fidmentioning

confidence: 99%

Cold-pressed pumpkin seed (<em>Cucurbita pepo</em> L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

et al. 2018

View full text Add to dashboard Cite

show abstract

“…polar cyanopropylsiloxane columns [23,27,28]. Gu et al [20] demonstrated the separation of a 37-component FAME test mixture on HP-5ms, IL82, IL100, and HP-88 columns, and evaluated the performance of separation of FAMEs as excellent on the IL100 column for separation of cis/trans-isomers.…”

Section: H/c Mdgc Analysis: C18 Famementioning

confidence: 98%

Integrated multidimensional and comprehensive 2D GC analysis of fatty acid methyl esters

Zeng

Chin

Marriott

2013

J of Separation Science

View full text Add to dashboard Cite

Fatty acid methyl ester (FAME) profiling in complex fish oil and milk fat samples was studied using integrated comprehensive 2D GC (GC × GC) and multidimensional GC (MDGC). Using GC × GC, FAME compounds--cis- and trans-isomers, and essential fatty acid isomers--ranging from C18 to C22 in fish oil and C18 in milk fat were clearly displayed in contour plot format according to structural properties and patterns, further identified based on authentic standards. Incompletely resolved regions were subjected to MDGC, with Cn (n = 18, 20) zones transferred to a (2)D column. Elution behavior of C18 FAME on various (2)D column phases (ionic liquids IL111, IL100, IL76, and modified PEG) was evaluated. Individual isolated Cn zones demonstrated about four-fold increased peak capacities. The IL100 provided superior separation, good peak shape, and utilization of elution space. For milk fat-derived FAME, the (2)D chromatogram revealed at least three peaks corresponding to C18:1, more than six peaks for cis/trans-C18:2 isomers, and two peaks for C18:3. More than 17 peaks were obtained for the C20 region of fish oil-derived FAMEs using MDGC, compared with ten peaks using GC × GC. The MDGC strategy is useful for improved FAME isomer separation and confirmation.

show abstract

“…Ionic liquids (ILs), due to their many unique properties such as low vapor pressure, high thermal stability, non-flammability and good solubility for inorganic and organic compounds, are emerging as desirable alternatives for traditional volatile organic solvents. Nowadays, ILs have been widely used in extraction processes [1][2][3], chemical synthesis and catalysis [4,5], chromatographic methods [6][7][8] and electrochemical applications [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we synthesized a hydrophobic amino-functionalized IL, 1-(2-aminoethyl)-3-butylimidazolium hexafluorophosphate ([NH 2 C 2 C 4 im] [PF 6 ]). The interaction behavior of [NH 2 C 2 C 4 im][PF 6 ] and copper(II) ion (Cu 2+ ) was investigated. The extractability of the amino-functionalized IL was evaluated by using Cu 2+ as a model metal ion.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Extraction behavior of copper(II) ion with a hydrophobic amino-functionalized ionic liquid

et al. 2012

View full text Add to dashboard Cite

We have prepared the hydrophobic aminofunctionalized ionic liquid (IL) 1-(2-aminoethyl)-3-butylimidazolium hexafluorophosphate and investigated its extraction behavior for copper(II) ion as a model cation. The IL, due to the presence of an amino group, is capable of complexing Cu(II) in a ratio of 6:1. The parameters affecting the extraction efficiency were optimized. The IL-based liquid-liquid microextraction was successfully applied to the analysis of Cu(II) in an environmental water standard reference material. The results are promising in terms of liquidliquid microextraction, separation, and preconcentration of Cu(II).

show abstract

Evaluation of ionic liquid stationary phases for one dimensional gas chromatography–mass spectrometry and comprehensive two dimensional gas chromatographic analyses of fatty acids in marine biota

Cited by 73 publications

References 40 publications

Cold-pressed pumpkin seed (<em>Cucurbita pepo</em> L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

Cold-pressed pumpkin seed (<em>Cucurbita pepo</em> L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

Integrated multidimensional and comprehensive 2D GC analysis of fatty acid methyl esters

Extraction behavior of copper(II) ion with a hydrophobic amino-functionalized ionic liquid

Contact Info

Product

Resources

About