Evaluation of lethality temperature and use of different wall materials in the microencapsulation process of Trichoderma asperellum conidias by spray drying

Braga, Alinne Brandão Andalécio Camargos; Costa, Cleiver Júnio Martins; Pomella, Alan William Vilela; Ribeiro, Eloízio Júlio; Santos, Líbia Diniz; Zotarelli, Marta Fernanda

doi:10.1016/j.powtec.2019.02.037

Cited by 20 publications

(7 citation statements)

References 60 publications

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“…Powder culture at 90 ºC demonstrated the highest growth in early incubation days before declining after day 10, which may indicate that the heating level had affected the cell's metabolisms. This trend was also shown by Trichoderma asperellum conidia when a reduction in survival percentage was observed at high temperatures (80-90 ºC) and the survival was mostly supported by the application of a protectant [4]. Powder culture at 80 ºC was able to sustain growth up to 12 days before reducing.…”

Section: Effect Of Powder Formation On Mycelium Growth Curvementioning

confidence: 52%

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Growth Revival of Grey Oyster (Pleurotus Pulmonarius) Powder Culture Mushroom From the Effect of Spray Drying Temperature

Zakaria,

Aziz,

Md Sarip

et al. 2024

IIUMEJ

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In mushroom cultivation, spawn is among the most important factors that ensure a successful production. Considering several drawbacks from both solid and liquid spawn in shelf life and preservation matters, it is suggested that the dry powder form of spawn is a good potential to explore. A study of powder culture formation using a spray dryer involved various inlet temperatures of 80 oC, 90 oC, 100 oC, 110 oC, 120 oC and 130 oC. The yield % of the dried powder culture was measured and it was found that 130 oC yielded the highest percentage of 50.33%. The lowest temperature yielded the lowest percentage. On the other hand, the lowest temperature of 80 oC revived the highest mycelium dry weight at 1.68 g which was obtained on the 10th day of the incubation periods. The revival ability was decreased with the increase of temperature. The study proved that the powder culture of P. pulmonarius was able to perform and revive whereby it holds a potential to be preserved over a longer period, which is beneficial for the mushroom cultivator. ABSTRAK: Dalam penanaman cendawan, benih yang baik adalah antara faktor penting yang memastikan penghasilan tinggi. Terdapat beberapa kelemahan daripada benih pepejal dan cecair dalam memastikan jangka hayat berpanjangan serta memastikan benih dalam keadaan berkualiti sepanjang masa. Oleh itu, penghasilan benih cendawan dalam bentuk serbuk kering sangat berpotensi untuk diterokai. Kajian mengenai penghasilan kultur serbuk menggunakan semburan kering telah melibatkan pelbagai suhu salur masuk seperti 80 oC, 90 oC, 100 oC, 110 oC, 120 oC dan 130 oC. Peratus penghasilan kultur serbuk kering telah diukur dan didapati pada suhu 130 oC menghasilkan peratusan tertinggi iaitu sebanyak 50.33%, manakala suhu terendah menghasilkan peratusan terendah. Sebaliknya, suhu terendah pada 80 oC menumbuhkan semula berat kering miselium dengan bacaan tertinggi iaitu sebanyak 1.68 g diperoleh pada hari ke-10 tempoh inkubasi. Keupayaan menumbuh berkurangan dengan peningkatan suhu. Kajian membuktikan bahawa kultur serbuk P. pulmonarius mampu berfungsi dan tumbuh semula di mana ianya berpotensi disimpan dalam tempoh lama, ini berfaedah kepada penanaman cendawan.

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Section: Effect Of Powder Formation On Mycelium Growth Curvementioning

confidence: 52%

“…The advantage of this type of spawn is that it can be dispersed equally [3]. Nevertheless, it is difficult to transport and store for long periods and is prone to contamination [4]. Considering several drawbacks from both solid and liquid spawn, it is suggested that other forms of spawn, such as in dry powder form, should be explored.…”

Section: Introductionmentioning

confidence: 99%

Growth Revival of Grey Oyster (Pleurotus Pulmonarius) Powder Culture Mushroom From the Effect of Spray Drying Temperature

Zakaria,

Aziz,

Md Sarip

et al. 2024

IIUMEJ

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“…These results are consistent with those reported in the literature for the same matrices. Braga et al investigated different matrices for encapsulating Trichoderma asperellum using a spray drying technique. The authors also found mean mass diameter values { d (0.5)}, between 10 and 40 μm, for the evaluated polymers.…”

Section: Resultsmentioning

confidence: 99%

Encapsulation of Beauveria bassiana in Polysaccharide-Based Microparticles: A Promising Carrier System for Biological Control Applications

de Oliveira,

Savassa

et al. 2023

ACS Agric. Sci. Technol.

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Beauveria bassiana is an entomopathogenic fungus that is widely used as a biocontrol agent against various insect pests in agriculture. However, its efficacy is limited by several factors such as ultraviolet radiation, high temperatures, and low humidity. These limitations can be overcome by microencapsulating B. bassiana, which protects the fungus and facilitates sustained release of the fungus. In the present study, we aimed to develop microencapsulated formulations of B. bassiana using a spray-drying technique and to evaluate their physicochemical properties, shelf life, and biological activity against two-spotted spider mites (Tetranychus urticae). Temperature and matrices influenced the physicochemical characteristics of B. bassiana formulations, with matrix mixing being the most promising strategy for more stable formulations. The formulations presented a mean size of 8−30 μm, indicating good potential for biocontrol applications. Biological assays demonstrated that the microencapsulated formulations increased the performance of B. bassiana against two-spotted spider mites, and even low conidial concentrations of B. bassiana in the formulations resulted in high mite mortality rates. These findings indicated that microencapsulation can improve the efficacy of B. bassiana as a biocontrol agent and overcome some of its limitations. Therefore, the development of microencapsulated formulations has promising prospects for use in integrated pest management programs, providing sustainable and environment-friendly alternatives to conventional pesticides.

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“…formulations. In each case, processing parameters, type of propagules, additives and spore viability are listed Drying technique Processing parameters Conidia species Additives Spore viability (%) Ref Spray drying Inlet/outlet temp 90/65 °C T. asperellum Maltodextrin DE20 93% after drying (Braga et al 2019 ) Spray drying Inlet/outlet temp. 80/50 °C T. harzianum - 25% after drying (Fernández-Sandoval et al 2012 ) Spray drying Inlet/outlet temp.…”

Section: Drying Techniquesmentioning

confidence: 99%

Biotechnological development of Trichoderma-based formulations for biological control

Martínez

Ribera

Schwarze

et al. 2023

Appl Microbiol Biotechnol

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Trichoderma spp. are a genus of well-known fungi that promote healthy growth and modulate different functions in plants, as well as protect against various plant pathogens. The application of Trichoderma and its propagules as a biological control method can therefore help to reduce the use of chemical pesticides and fertilizers in agriculture. This review critically discusses and analyzes groundbreaking innovations over the past few decades of biotechnological approaches to prepare active formulations containing Trichoderma. The use of various carrier substances is covered, emphasizing their effects on enhancing the shelf life, viability, and efficacy of the final product formulation. Furthermore, the use of processing techniques such as freeze drying, fluidized bed drying, and spray drying are highlighted, enabling the development of stable, light-weight formulations. Finally, promising microencapsulation techniques for maximizing the performance of Trichoderma spp. during application processes are discussed, leading to the next-generation of multi-functional biological control formulations. Key points • The development of carrier substances to encapsulate Trichoderma propagules is highlighted. • Advances in biotechnological processes to prepare Trichoderma-containing formulations are critically discussed. • Current challenges and future outlook of Trichoderma-based formulations in the context of biological control are presented.

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Evaluation of lethality temperature and use of different wall materials in the microencapsulation process of Trichoderma asperellum conidias by spray drying

Cited by 20 publications

References 60 publications

Growth Revival of Grey Oyster (Pleurotus Pulmonarius) Powder Culture Mushroom From the Effect of Spray Drying Temperature

Growth Revival of Grey Oyster (Pleurotus Pulmonarius) Powder Culture Mushroom From the Effect of Spray Drying Temperature

Encapsulation of Beauveria bassiana in Polysaccharide-Based Microparticles: A Promising Carrier System for Biological Control Applications

Biotechnological development of Trichoderma-based formulations for biological control

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