Evaluation of Mannitol Salt Agar for Detection of Oxacillin Resistance in
            <i>Staphylococcus aureus</i>
            by Disk Diffusion and Agar Screening

Kampf, Günter; Lecke, Christoph; Cimbal, Ann-Katrin; Weist, Klaus; Rüden, H.

doi:10.1128/jcm.36.8.2254-2257.1998

Cited by 24 publications

(6 citation statements)

References 32 publications

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“…As per previous research, Staphylococcus aureus is the most common organism responsible for orthopaedic infections [ 16 ]. Mannitol salt agar (MSA) has been used as a selective medium for the isolation of pathogenic staphylococci since 1945; hence, Mannitol Salt Agar (MSA) was also used in addition to MAC and BA [ 17 , 18 ]. Bacterial growth was studied after incubating the culture plates aerobically for 24 hours at 37.0°C.…”

Section: Methodsmentioning

confidence: 99%

Current pathogens infecting open fracture tibia and their antibiotic susceptibility at a tertiary care teaching hospital in South East Asia

Islam

Sultana³

et al. 2022

Infection Prevention in Practice

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Section: Methodsmentioning

confidence: 99%

Current pathogens infecting open fracture tibia and their antibiotic susceptibility at a tertiary care teaching hospital in South East Asia

Islam

Sultana³

et al. 2022

Infection Prevention in Practice

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“…Growth of yellow coloured colonies indicated the presence of Staphylococcus organisms. The test was performed following the procedure of Kampf et al, (1998) with certain modifications. The isolates were stored in 50% glycerol solution in eppendroff tubes for further use.…”

Section: Isolation and Identification Of Staphylococcus Aureusmentioning

confidence: 99%

Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

Ramasamy¹,

Keerthana²,

Srinivasan³

et al. 2020

IJAR

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Background: Antimicrobial resistance is one of the latest challenges facing the scientific community. Raising the drug resistance is caused mainly by indiscriminate usage of antibiotics in human and animal subjects and the spread of antibiotic resistance between the two has an emerging global threat. Hence, current study aimed to study the antimicrobial resistance pattern and molecular detection of antibiotic resistance genes in Staphylococcus aureus and Escherichia coli isolated from mastitis affected cows. Methods: Milk samples from mastitis affected cows were subjected to antibiotic sensitivity test and screened for presence of Staphylococcus aureus and Escherichia coli using differential growth media. Molecular characterization of Staphylococcus aureus and Escherichia coli was done with the help of PCR by amplification of ‘nuc’ and ‘uspA’ gene respectively. MICs of Penicillin and Tetracycline were determined using microdilution method. Result: Antibiotic sensitivity pattern for Penicillin G, Ampicillin, Amoxycillin, Cefotaxime, Ceftriaxone, Azithromycin, Ciprofloxacin, Gentamicin, Oxytetracycline, Tetracycline and Vancomycin were 74.19%, 100%, 93.50%, 61.29%, 29%, 35.48%, 9.70%, 9.70%, 70.96% and 70.96% respectively. More than 87.90% of the S. aureus and 50% of the E. coli isolated were resistant to â-lactam antibiotics while 75% of the E.coli and 65.70% of the S. aureus isolated were resistant to Tetracycline antibiotics. The MICs of Penicillin for S.aureus and E.coli are 26.88 µg/ml and 13.54 µg/ml respectively and the MICs of Tetracycline for S. aureus and E. coli are 243.75 µg/ml and 960.93 µg/ml respectively which is 8-9 folds higher than the standard MICs. From the present study, it can be inferred that bovine mastitis cases are highly resistant to antimicrobial drugs. Results further indicate that Staphylococcus aureus and Escherichia coli are both resistant to Penicillin and Tetracycline with very high MIC.

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“…Em seguida, os testes foram avaliados quanto a sua sensibilidade (Tabela 2), uma vez que a presença do gene mecA foi considerada um "padrão ouro" para detecção das espécies resistentes. Os valores obtidos foram submetidos à análise estatística (IHAKA, R. & GENTLEMAN, R., 1996), na qual foi possível concluir que houve diferença significativa KAMPF et al (1998) e GERBERDING et al (1991. A difusão em disco modificada foi o método que apresentou menor sensibilidade (61,5%).…”

Section: Staphylococcus Aureus Dos 59 Isolados De Amostras Animais unclassified

Mapeamento do perfil de resistência e detecção do gene mecA em Staphylococcus aureus e Staphylococcus intermedius oxacilina-resistentes isolados de espécies humanas e animais

et al. 2007

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Espécies de Staphylococcus spp resistentes a antimicrobianos representam um problema cosmopolita, sendo o controle de sua disseminação um importante desafio. O perfil de resistência a antimicrobianos de isolados de Staphylococcus aureus e Staphylococcus intermedius em amostras clínicas humanas e animais foi avaliado através do método de difusão em disco, no qual foi possível detectar um elevado nível de resistência à ampicilina e à penicilina. A avaliação da resistência à oxacilina, devido à heterogeneidade de resposta do gênero estudado, foi desenvolvida também através das seguintes técnicas: difusão em ágar modificado, ágar screen e microdiluição em caldo, e posteriormente correlacionada com a detecção do gene mecA, pela técnica de PCR, nas amostras consideradas resistentes em pelo menos um dos testes utilizados. A correlação entre os resultados obtidos nos testes fenotípicos com a presença do gene de resistência, considerado um método de referência, foi utilizada para validar a sensibilidade destes. De um total de 80 amostras avaliadas, 28 apresentaram resistência à oxacilina, sendo possível detectar a presença do gene de resistência mecA em 12 destas amostras. Os testes de suscetibilidade à oxacilina apresentaram sensibilidade superior a 50,0%, sendo a difusão em disco simples e o ágar screen considerados mais sensíveis, e a difusão em disco modificada, o de menor sensibilidade.

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Evaluation of Mannitol Salt Agar for Detection of Oxacillin Resistance in Staphylococcus aureus by Disk Diffusion and Agar Screening

Cited by 24 publications

References 32 publications

Current pathogens infecting open fracture tibia and their antibiotic susceptibility at a tertiary care teaching hospital in South East Asia

Current pathogens infecting open fracture tibia and their antibiotic susceptibility at a tertiary care teaching hospital in South East Asia

Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

Mapeamento do perfil de resistência e detecção do gene mecA em Staphylococcus aureus e Staphylococcus intermedius oxacilina-resistentes isolados de espécies humanas e animais

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