Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Clinically Important Yeast Species

Stevenson, Lindsay; Drake, Steven K.; Shea, Yvonne R.; Zelazny, Adrian M.; Murray, Patrick R.

doi:10.1128/jcm.00687-09

Cited by 216 publications

(199 citation statements)

References 30 publications

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“…Yet, direct identification of environmental yeast isolates by MALDI-TOF MS is still limited. As identification successes obtained for clinical yeast isolates are very promising (Marklein et al 2009;Stevenson et al 2010;van Veen et al 2010;Dhiman et al 2011), an expansion of the database toward environmental isolates is desirable. With these data becoming available, it will be possible to fully exploit the capacity of this rapid and costeffective method for environmental studies.…”

Section: Yeast Identification and Classification By Maldi-tof Msmentioning

confidence: 99%

“…Furthermore, reference spectra created from measurements of one isolate can be compared with those of other isolates to analyze phylogenetic relationships and thus classify unknown organisms. This mass spectrometric technique has been extensively applied to the study of clinically important yeasts, yielding high rates of correct species-level identifications (e.g., Marklein et al 2009;Stevenson et al 2010;van Veen et al 2010;Dhiman et al 2011). Yet, to our knowledge, it has not been applied to yeasts in the context of ecological studies.…”

Section: Introductionmentioning

confidence: 99%

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Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

et al. 2013

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Marine yeasts play an important role in biodegradation and nutrient cycling and are often associated with marine flora and fauna. They show maximum growth at pH levels lower than present-day seawater pH. Thus, contrary to many other marine organisms, they may actually profit from ocean acidification. Hence, we conducted a microcosm study, incubating natural seawater from the North Sea at present-day pH (8.10) and two near-future pH levels (7.81 and 7.67). Yeasts were isolated from the initial seawater sample and after 2 and 4 weeks of incubation. Isolates were classified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) and representative isolates were identified by partial sequencing of the large subunit rRNA gene. From the initial seawater sample, we predominantly isolated a yeast-like filamentous fungus related to Aureobasidium pullulans, Cryptococcus sp., Candida sake, and various cold-adapted yeasts. After incubation, we found more different yeast species at near-future pH levels than at present-day pH. Yeasts reacting to low pH were related to Leucosporidium scottii, Rhodotorula mucilaginosa, Cryptococcus sp., and Debaryomyces hansenii. Our results suggest that these yeasts will benefit from seawater pH reductions and give a first indication that the importance of yeasts will increase in a more acidic ocean.

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Section: Yeast Identification and Classification By Maldi-tof Msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

et al. 2013

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“…First, samples can be prepared directly from positive blood cultures [45]. Secondly, there may be more information to be gained from the same assays because the data currently utilized are only a fraction of the available signal, albeit that fraction with the lowest confounding noise relative to the gold standards of identification.…”

Section: Traumatic Wound Microbiome Workhop 845mentioning

confidence: 99%

Traumatic Wound Microbiome Workshop

et al. 2012

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“…В настоящем исследовании для верификации данных бактериологической идентификации штаммов S. aureus использован новый молекулярный подход, получивший название прямого масс-спектрометрического профилирования. Метод прямого масс-спектрометрического профилирования зарекомендовал себя, как надежный и воспроизводимый метод, используемый для быстрой идентификации клинических изолятов бактерий и грибов [15,18].…”

Section: рисунокunclassified

Strain differentiation of staphylococcus aureus by means of direct maldi tof mass spectrometry profiling

et al. 2012

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Staphylococcus aureus - one of the most interesting for clinical studies of microbial species with extensive strain diversity, primarily due to the variability of virulence factors and pathogenicity. The aim of this study was approbation of a method for the rapid strain differentiation of S. aureus on the basis of bacterial cell direct protein profiling approach by means of MALDI TOF MS. Beta-lactamase and alpha-hemolysin productions, cording by the blaZ and hla genes, respectively, were selected as markers for the strain differentiation. Mathematical analysis of MALDI mass spectra from 53 isolates allowed the construction of two independent classification models that can differentiate the strains on the presence/absence of blaZ or hla genes. A number of the most significant peaks (masses), which can be considered as markers of the strain differences in S. aureus, were identified using a statistical contribution of each mass peak in the models. These diagnostic models differ the sensitivity and the specificity, which were 97.5% and 82.5% for the classification of strains on the basis of beta-lactamase production, and 90.0% and 88.7% by the presence of alpha-hemolysin.

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Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Clinically Important Yeast Species

Cited by 216 publications

References 30 publications

Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

Traumatic Wound Microbiome Workshop

Strain differentiation of staphylococcus aureus by means of direct maldi tof mass spectrometry profiling

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