Evaluation of methods to extract and quantify lipids from Synechocystis PCC 6803

Sheng, Jie; Vannela, Raveender; Rittmann, Bruce E.

doi:10.1016/j.biortech.2010.08.007

Cited by 167 publications

(129 citation statements)

References 33 publications

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“…The total membrane lipids were extracted by Folch method (18) and transesterified into fatty acid methyl ester by methanolic HCl (Supelco). The fatty acid profile of the total lipids in 6803 wild type was analyzed by GC (19).…”

Section: Methodsmentioning

confidence: 99%

CO ₂ -limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass

Liu

Fallon

Sheng

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Using genetically modified cyanobacterial strains, we engineered a Green Recovery strategy to convert membrane lipids into fatty acids for economical and environmentally sustainable biofuel production. The Green Recovery strategy utilizes lipolytic enzymes under the control of promoters induced by CO 2 limitation. Data indicate that strains of the cyanobacterium Synechocystis sp. PCC6803 engineered for Green Recovery underwent degradation of membrane diacylglycerols upon CO 2 limitation, leading to release of fatty acids into the culture medium. Recovered fatty acid yields of 36.1 × 10 -12 mg/cell were measured in one of the engineered strains (SD239). Green Recovery can be incorporated into previously constructed fatty-acid-secretion strains, enabling fatty acid recovery from the remaining cyanobacterial biomass that will be generated during fatty acid biofuel production in photobioreactors.

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Section: Methodsmentioning

confidence: 99%

CO ₂ -limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass

Liu

Fallon

Sheng

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…S9; López et al, 2013). The present model used 16 different lipid types corresponding to the four major groups present in cyanobacteria: phosphatidylglycerol (PG), monogalactosyl-diacylglycerol (MGDG), digalactosyl-diacylglycerol (DGDG), and sulfoquinovosyldiacylglycerol (SQDG), in a ratio as experimentally determined in Synechocystis (Supplemental Table S6; Sheng et al, 2011). The system contained a total of 2400 lipids, resulting in a large membrane slab with dimensions of approximately 21 3 27 nm.…”

Section: The Absence Of Hydrocarbons Has Minor Effects On Photosynthementioning

confidence: 99%

“…The in silico cyanobacterial membrane lipid compositions were based on the experimental lipid extractions and characterization of Synechocystis by Sheng et al (2011). Four major classes of cyanobacterial lipids were used: PG, MGDG, DGDG, and SQDG, with various acyl tails differing in length and degree of saturation.…”

Section: Modeling Of Synechocystis Membranesmentioning

confidence: 99%

“…Standard bonded parameters were used. The compositions determined by Sheng et al (2011) are shown in Supplemental Table S6, and compared to the number of lipids used in our model membranes to reproduce as closely as possible this composition. The hydrocarbon heptadecane was added in varying quantities to study the effects of this compound on membrane properties.…”

Section: Modeling Of Synechocystis Membranesmentioning

confidence: 99%

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Hydrocarbons Are Essential for Optimal Cell Size, Division, and Growth of Cyanobacteria

et al. 2016

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Cyanobacteria are intricately organized, incorporating an array of internal thylakoid membranes, the site of photosynthesis, into cells no larger than other bacteria. They also synthesize C15-C19 alkanes and alkenes, which results in substantial production of hydrocarbons in the environment. All sequenced cyanobacteria encode hydrocarbon biosynthesis pathways, suggesting an important, undefined physiological role for these compounds. Here, we demonstrate that hydrocarbon-deficient mutants of Synechococcus sp. PCC 7002 and Synechocystis sp. PCC 6803 exhibit significant phenotypic differences from wild type, including enlarged cell size, reduced growth, and increased division defects. Photosynthetic rates were similar between strains, although a minor reduction in energy transfer between the soluble light harvesting phycobilisome complex and membrane-bound photosystems was observed. Hydrocarbons were shown to accumulate in thylakoid and cytoplasmic membranes. Modeling of membranes suggests these compounds aggregate in the center of the lipid bilayer, potentially promoting membrane flexibility and facilitating curvature. In vivo measurements confirmed that Synechococcus sp. PCC 7002 mutants lacking hydrocarbons exhibit reduced thylakoid membrane curvature compared to wild type. We propose that hydrocarbons may have a role in inducing the flexibility in membranes required for optimal cell division, size, and growth, and efficient association of soluble and membrane bound proteins. The recent identification of C15-C17 alkanes and alkenes in microalgal species suggests hydrocarbons may serve a similar function in a broad range of photosynthetic organisms.

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“…It should be noticed that obtaining lipid is a prerequisite for the production of microalgal fuel. More and more investigations have showed that microalgae are potentially good biomass materials for development of clean green energy [28][29][30][31][32][33][34][35][36][37][38][39][40] .…”

Section: Introductionmentioning

confidence: 99%

Optimalization of Extraction Conditions for Increasing Microalgal Lipid Yield by Using Accelerated Solvent Extraction Method (ASE) Based on the Orthogonal Array Design

Rulong¹,

Wenxuan²,

Xing³

et al. 2012

Energy Conservation

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Evaluation of methods to extract and quantify lipids from Synechocystis PCC 6803

Cited by 167 publications

References 33 publications

CO ₂ -limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass

CO ₂ -limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass

Hydrocarbons Are Essential for Optimal Cell Size, Division, and Growth of Cyanobacteria

Optimalization of Extraction Conditions for Increasing Microalgal Lipid Yield by Using Accelerated Solvent Extraction Method (ASE) Based on the Orthogonal Array Design

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Evaluation of methods to extract and quantify lipids from Synechocystis PCC 6803

Cited by 167 publications

References 33 publications

CO 2 -limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass

CO 2 -limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass

Hydrocarbons Are Essential for Optimal Cell Size, Division, and Growth of Cyanobacteria

Optimalization of Extraction Conditions for Increasing Microalgal Lipid Yield by Using Accelerated Solvent Extraction Method (ASE) Based on the Orthogonal Array Design

Contact Info

Product

Resources

About

CO ₂ -limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass

CO ₂ -limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass