Evaluation of microalgae cell disruption by ultrasonic treatment

Gerde, José A.; Montalbo‐Lomboy, Melissa; Yao, Linxing; Grewell, David; Wang, Tong

doi:10.1016/j.biortech.2012.08.110

Cited by 189 publications

(113 citation statements)

References 33 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…Thus, if the original metabolites endured varied degrees of degradation during US treatment to generate new species of products that have much higher or much lower UV absorbance, errors became inevitable. Some cellular metabolites, such as lipids, may form micelles by adhering to the cell debris to form particles and thus separate from the aqueous medium, which would cause UV absorbance under estimation [30].…”

Section: Effectiveness Of Ultrasoundmentioning

confidence: 99%

Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

et al. 2016

View full text Add to dashboard Cite

This study evaluated the effectiveness of pressure-assisted ozonation (PAO) in Chlorella vulgaris (C. vulgaris) cell disruption, and compared the disruption result with that of the ultrasonication (US) by using four quantification indicators: cell counting, ultra violet (UV) absorbance, turbidity and visible light absorbance. It was found that under the condition of 0.8 MPa and 80 cycles, PAO treatment achieved cell rupture of 80.3%, with the power of 1080 W and treatment time of 60 min, US achieved cell rupture of 83.8%. Cell counting was a reliable indicator and applicable to both PAO and US treatments. Turbidity and visible light absorbance gave similar results and featured as the simplest operation. UV absorbance reflected the metabolite release due to cell breakage; however, it was less reproducible when it was applied to quantify the cell rupture by PAO. Its trend indicated that during cell disruption metabolite degradation occurred, especially after significant rupture in the case of excessive PAO treatment. The cellular morphology of C. vulgaris cells during PAO and US treatments was investigated by scanning electron microscope (SEM) which certified that the cells damage was caused by both physical and chemical attack.

show abstract

Section: Effectiveness Of Ultrasoundmentioning

confidence: 99%

Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The amount of chlorophyll extracted from the microalgae has been correlated with cell wall disruption by Gerde et al (2012). The total chlorophyll A and C obtained after the in situ transesterification by the different catalysts was measured using a modified version of the method previously described by Gerde et al (2012).…”

Section: Quantification Of Cell Disruption After In Situ Transesterifmentioning

confidence: 99%

“…The total chlorophyll A and C obtained after the in situ transesterification by the different catalysts was measured using a modified version of the method previously described by Gerde et al (2012). To study the extent of cell disruption in N. occulata, 0.47 mL of methanol was added to a 100 mg of dried microalgae in a 2.5 mL tube followed by the addition of 100 % H2SO4 (w/w oil).…”

Section: Quantification Of Cell Disruption After In Situ Transesterifmentioning

confidence: 99%

Surfactant-assisted direct biodiesel production from wet Nannochloropsis occulata by in situ transesterification/reactive extraction

2016

View full text Add to dashboard Cite

Please cite this article as: Salam K.A., Velasquez-Orta S.B., Harvey A.P. Effect Surfactant-assisted direct biodiesel production from wet Nannochloropsis occulata by in situ transesterification/reactive extraction. Biofuel Research Journal 9 (2016) HIGHLIGHTSSurfactant assisted in situ transesterification of wet algae was studied.A surfactant catalyst ("ZDS") produced high yields in Nannochloropsis occulata.Inclusion of SDS in increased FAME production in the wet algae.The process was not adversely affected by water in the algae up to 20%. This article reports an in situ transesterification/reactive extraction of Nannochloropsis occulata for fatty acid methyl ester (FAME) production using H2SO4, sodium dodecyl sulphate (SDS) plus H2SO4 and zirconium dodecyl sulphate (ZDS). A maximum 67 % FAME yield was produced by ZDS. Effect of inclusion of sodium dodecyl sulphate (SDS) in H2SO4 for FAME enhancement and water tolerance was also studied by hydrating the algae with 10 % -30 % distilled water (w/w) dry algae. Treatment with SDS in H2SO4 increases the FAME production rate and water tolerance of the process. Inclusion of SDS in H2SO4 produced a maximum 98.3 % FAME yield at 20 % moisture in the algae. The FAME concentration began to diminish only at 30 % moisture in the algae. Furthermore, the presence of a small amount of water in the biomass or methanol increased the lipid extraction efficiency, improving the FAME yield, rather than inhibiting the reaction. ARTICLE INFO ABSTRACT

show abstract

“…O método combinado foi eficiente para a extração da enzima em escala laboratorial. Gerde et al (2012) utilizaram o método de extração ultrassônico para extração de lipídeos do interior da célula de microalgas autotróficas e heterotróficas, o qual se mostrou eficiente. Em ambos os tipos de algas, a extração de material intracelular aumentou com o aumento do tempo e da energia dissipada.…”

Section: Bbr -Biochemistry and Biotechnology Reportsunclassified

Extração de B-galactosidase de Kluyveromyces marxianus CCT 7082 por método ultrassônico

Lemes

Álvares

Kalil

2013

BBR

View full text Add to dashboard Cite

The β-galactosidase is an intracellular enzyme obtained from the yeast Kluyveromyces marxianus and its role is hydrolyse lactose and therefore, is important in the dairy industry. However, for their effective use is necessary to better understand the extraction methods available. The ultrasonic disruption method has been applied as a separation step of intracellular products on laboratory scale and consists of ultrasonic waves dissipation in the solution through cavitation bubbles and produce velocity gradient that creates a force capable of breaking the cells. The aim of this study was to evaluate the rupture ultrasonic extraction of the enzyme β-galactosidase obtained from the yeast Kluyveromyces marxianus CCT 7082 compared with abrasion method using glass beads. The method using ultrasonic ruptor (150 W) with an intermediate tip (70% power) was effective for cell disruption of yeast, showing high enzyme activity and yield, similar values to the extraction method using abrasive glass beads.

show abstract

Evaluation of microalgae cell disruption by ultrasonic treatment

Cited by 189 publications

References 33 publications

Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

Surfactant-assisted direct biodiesel production from wet Nannochloropsis occulata by in situ transesterification/reactive extraction

Extração de B-galactosidase de Kluyveromyces marxianus CCT 7082 por método ultrassônico

Contact Info

Product

Resources

About