Evaluation of microbial communities in peels of Brazilian tropical fruits by amplicon sequence analysis

Abstract

Elucidation of the distinctive microbial taxonomic profiles of tropical fruit peels is the indispensable component of investigations aimed at the detection of microorganisms responsible for the post-harvest loss. The objective of the present work was to dissect the bacterial and fungal community of five tropical fruit peels (banana, guava, mango, papaya, and passion fruit) in wild (noncultivated) and conventionally produced samples from Brazil. To that end, 16S rRNA-encoding gene and ITS rDNA amplicon analysis of the five tropical fruit peels were performed to discriminate the bacterial and fungal communities, respectively. The result showed that bacterial communities of the five types of fruit peels were by far more diversified than that of fungal communities, independent of the type of production system involved. Among the investigated fruits, non-cultivated papaya peels hosted the most diversified bacterial community while the least bacterial community diversity was found in the conventionally produced papaya fruit peels. The gene amplicon analysis clearly discriminated the bacterial community into their respective classes, while fungal communities were better classified in their phyla, yet with clearer component discrimination of fungal community based on the type of cultivation system practiced. Conventionally produced banana and non-cultivated passion fruit peels were characteristically dominated by fungal and bacterial groups, respectively. Overall, in conventionally produced fruit peels, bacterial community was mainly composed of Proteobacteria, Actinobacteria, and Bacilli. The result provided a broad microbial diversity profile that could be used as an important input for seeking alternative fruit spoilage control and post-harvest treatments.

Diversity of the microbial community and antioxidant activity during fermentation of red raspberry Enzymes

“…Alpha diversity (Chao1 richness and Ace richness estimators, Simpson and Shannon diversity indices) was evaluated using the Mothur v.1.11.0 program, and beta diversity (principal component analysis, UPGMA, heat map) was performed using QIIME software. Ultimately, redundancy analysis (RDA) and canonical correspondence analysis (CCA) were performed using Canoco software to reflect the relationship between the core microorganisms and antioxidant activity (Cruz et al., 2019).…”

Use of Liquid Organic Fertilizer from Kepok Banana Peel (Musa acuminata) on the Growth of Mustard Plants (Brassica juncea)

“…Hasil uji ANOVA menunjukkan bahwa pada tinggi tanaman dan berat basah tanaman terdapat adanya beda nyata. Selain karena adanya unsur hara yang terkandung dalam kulit pisang, menurut Cruz et al, (2019) kulit pisang ternyata memiliki bakteri Actinobakteria yang mengandung senyawa bioaktif yang bermanfaat untuk pertumbuhan tanaman. Diketahui pula bahwa kulit pisang mengandung hormon giberelin dari asam giberelat yang memicu pemanjangan batang dan pematangan daun (Advinda, 2018;Omojasola & Adejoro, 2018).…”

Bacterial Community Structure in Sago Pith and Sago Waste Water and Its Potential Uses as Organic Acids Producer

“…Research on bacterial communities in plants using metagenomebased approaches has been carried out on peels of banana, guava, mango, papaya, and passion fruit. [12], tomato [13,14], apple [15], watermelon [8], grapes, lettuce, mushrooms, spinach, sprouts, strawberries, peaches, and peppers [16]. Research on the bacterial community in sago plants using metagenome-based approaches has not been done.…”