Evaluation of miRNA-21 and miRNA Let-7 as Prognostic Markers in Patients With Breast Cancer

Elghoroury, Eman A.; ElDine, Hesham G.; Kamel, Solaf; Abdelrahman, Amany H.; Mohammed, Asmaa; Kamel, Mahmoud M.

doi:10.1016/j.clbc.2017.11.022

Cited by 66 publications

(43 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In BG circulating miRNA-21 tends to be higher in menopause subjects, which was in line with previous findings [25] Separated analysis on HG revealed a significant decrease of circulating miRNA-21 expression in menopause women. This finding might indicate that circulating miRNA-21 expression tends to decrease after menopause in normal settings.…”

supporting

confidence: 91%

Early detection breast cancer: role of circulating plasma miRNA-21 expression as a potential screening biomarker

Diansyah¹,

Ashariati²,

Sedana³

et al. 2021

Turk J Med Sci

View full text Add to dashboard Cite

Aim: To explore the circulating plasma miRNA-21 potential as an early detection biomarker by comparing early-stage breast cancer (BG) and healthy control (HG) in Indonesian population. Materials and methods: The enlisted patients were twenty-six adult female early-stage breast cancer patients (stage 1A, 1B, 2A, and 2B) of Airlangga University Hospital from August until October 2019. Sixteen volunteers were recruited as matched healthy subjects. MiRNA-21 expression was quantified by plasma qRT-PCR. Data analysis performed using IBM SPSS Statistics v.24. MiRNA-21 cutoff , sensitivity, and specificity were analyzed using receiver operating characteristic (ROC) curve. Results: The subject includes 26 BG and 16 HG subjects. The miRNA-21 expression in BG group was 3.933 (1.181-11.794) and 0.905 (0.164-4.532) in HG group (4.34 folds; p=0.001), with 1.66 cutoff (92.3% sensitivity; 81.2% specificity). MiRNA-21 expression separated analysis in HG showed a 0.578 times lower expression in menopause subjects [0.651 (0.164-0.414)] compared to pre-menopause [(1.123 (0.758-4.532); p=0.031]. Yet, in BG group 1.729 times higher miRNA-21 expression was observed in menopause subjects (6.021±3.583) compared to pre-menopause subjects (3.500±1.517;p=0.022). Conclusion: Circulating miRNA-21 expression is a potential biomarker for early detection of breast cancer and might act as a breast cancer risk predictor.

show abstract

supporting

confidence: 91%

Early detection breast cancer: role of circulating plasma miRNA-21 expression as a potential screening biomarker

Diansyah¹,

Ashariati²,

Sedana³

et al. 2021

Turk J Med Sci

View full text Add to dashboard Cite

show abstract

“…13 Furthermore, the expressions of serum miR-21 and miR-134 are increased in patients with BC and their levels could be used as promising markers for cancer diagnosis. 15 These findings confirm the necessity for further identification of key miRNAs involved in BC development. 15 These findings confirm the necessity for further identification of key miRNAs involved in BC development.…”

Section: Introductionsupporting

confidence: 56%

Retracted: Downregulation of microRNA‐1469 promotes the development of breast cancer via targeting HOXA1 and activating PTEN/PI3K/AKT and Wnt/β‐catenin pathways

Zhang

Fang

Zhao

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Breast cancer (BC) is a common malignancy which is the most frequently diagnosed cancer in women all over the worldwide. This study aimed to investigate the roles of miR‐1469 in the development of BC, as well as its regulatory mechanism. The expression levels of miR‐1469 in BC tissues, serum, and cell lines were determined. Effects of overexpression of miR‐1469 on MCF7 cell viability, colony‐forming ability, apoptosis, migration, and invasion were then investigated. Furthermore, the potential target of miR‐1469 in MCF7 cells was explored. Besides, the association between miR‐1469, PTEN/PI3K/AKT, and Wnt/β‐catenin pathways was elucidated. Notably, confirmatory experiments by downregulation of miR‐1469 in SK‐BR‐3 cells were further performed. The miR‐1469 expression was significantly downregulated in BC tissues, serum, and cell lines. The overexpression of miR‐1469 significantly inhibited the proliferation, arrested cell‐cycle at G2/M phase, increased apoptosis, suppressed migration, and invasion of MCF‐7 cells. In addition, HOXA1 was verified as a direct target of miR‐1469, and the effects of overexpression of miR‐1469 on the malignant behaviors of MCF7 cells were significantly counteracted by overexpression of HOXA1 concurrently. Furthermore, the overexpression of miR‐1469 suppressed the activation of PTEN/PI3K/AKT and Wnt/β‐catenin pathways, which was reversed overexpression of HOXA1 concurrently. Besides, confirmatory experiments showed that the inhibition of miR‐1469 promoted the malignant behaviors of SK‐BR‐3 cells, which was inversed after miR‐1469 inhibition and HOXA1 knockdown at the same time. Our findings reveal that downregulation of miR‐1469 may promote the development of BC by targeting HOXA1 and activating PTEN/PI3K/AKT and Wnt/β‐catenin pathways. MiR‐1469 may serve as a promising target for BC therapy.

show abstract

“…miRNAs are differentially expressed in cancer and play important roles in regulating gene expression by base-pairing to the complementary sites on the target mRNAs, consequently blocking translation or triggering the degradation of the target mRNAs [ 6 , 7 ]. Growing evidence has demonstrated the role of miRNAs in many human tumors, including breast [ 8 ], ovarian [ 9 ], pancreatic [ 10 ], thyroid [ 11 ], and lung cancers [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

HSP90: A Novel Target Gene of miRNA-628-3p in A549 Cells

Pan

Jiang

Zhou

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

Lung cancer is one of the leading causes of cancer-related death in the world. MicroRNA- (miR-) 628-3p plays critical roles in many cancers, including lung cancer. We investigated how miR-628-3p affected migration and apoptosis in A549 cells. We used bioinformatics algorithms to predict the miR-628-3p target gene to study the molecular mechanism by which miR-628-3p contributes to lung cancer. Then, we used the luciferase reporter assay to identify whether heat shock protein 90a (HSP90) is a direct target of miR-628-3p. Western blotting and quantitative real-time PCR showed that miR-628-3p downregulated HSP90a protein expression via a posttranscriptional mechanism. We confirm that miR-628-3p promotes apoptosis and inhibits migration in A549 cells by negatively regulating HSP90. Our results may reveal a novel strategy for lung cancer treatment.

show abstract

Evaluation of miRNA-21 and miRNA Let-7 as Prognostic Markers in Patients With Breast Cancer

Cited by 66 publications

References 21 publications

Early detection breast cancer: role of circulating plasma miRNA-21 expression as a potential screening biomarker

Early detection breast cancer: role of circulating plasma miRNA-21 expression as a potential screening biomarker

Retracted: Downregulation of microRNA‐1469 promotes the development of breast cancer via targeting HOXA1 and activating PTEN/PI3K/AKT and Wnt/β‐catenin pathways

HSP90: A Novel Target Gene of miRNA-628-3p in A549 Cells

Contact Info

Product

Resources

About