2017
DOI: 10.1016/j.jviromet.2017.01.008
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Evaluation of multiplex assay platforms for detection of influenza hemagglutinin subtype specific antibody responses

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Cited by 11 publications
(14 citation statements)
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“…Typically ≥ 4‐fold rise in HI and/or MN titers from paired sera collection indicates a positive antibody response to recent influenza infection or vaccination . In the multiplex MAGPIX assay, previously we used ≥ 2‐fold rise in MFI as the cutoff to determine serologic responses from paired sera, and the influenza viruses with the highest fold rise in MFI correlated with viruses of potential exposure . In the current study, no seroconversions (≥4‐fold rise) were detected by HI/MN for paired sera collected from A(H5N1) study.…”
Section: Resultsmentioning
confidence: 56%
“…Typically ≥ 4‐fold rise in HI and/or MN titers from paired sera collection indicates a positive antibody response to recent influenza infection or vaccination . In the multiplex MAGPIX assay, previously we used ≥ 2‐fold rise in MFI as the cutoff to determine serologic responses from paired sera, and the influenza viruses with the highest fold rise in MFI correlated with viruses of potential exposure . In the current study, no seroconversions (≥4‐fold rise) were detected by HI/MN for paired sera collected from A(H5N1) study.…”
Section: Resultsmentioning
confidence: 56%
“…The reliability and high-throughput of BLI platforms can assist in a multitude of vaccine research and immunotherapeutics applications. Recently, Li et al [ 25 ] found its use for serologic surveys and seroepidemiologic investigations for the identification of influenza hemagglutinin (HA) subtypes specific antibody responses. The influenza A virus HA glycoprotein is important during initial stages of infection, in which it is responsible for binding the virus to sialic acid present on the host cell membrane [ 26 ].…”
Section: Antibody Design and Antibody Capture Strategiesmentioning
confidence: 99%
“…Traditional serological surveys are performed by hemagglutination inhibition (HI) and virus microneutralization (MN) assays [ 27 , 28 ]. When evaluating potential new technologies, Li et al [ 25 ] found that BLI presents high sensitivity and expected specificity for human sera from patients exposed or unexposed to heterologous subtype influenza HA.…”
Section: Antibody Design and Antibody Capture Strategiesmentioning
confidence: 99%
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“…From serological tests, the most commonly used are hemagglutination inhibition assay (HAI), microneutralization/virus neutralization assay (VN), single radial hemolysis (SRH), complement fixation assay, enzyme-linked immunosorbent assay (ELISA) and Western blotting [55]. These kinds of tests are generally not recommended because of paired serum samples necessity.…”
Section: Influenza Virus Conventional Detection Methodsmentioning
confidence: 99%