Evaluation of New Genetic Toolkits and Their Role for Ethanol Production in Cyanobacteria

Gundolf, R.; Oberleitner, Sandra; Richter, Juliane

doi:10.3390/en12183515

Cited by 7 publications

(4 citation statements)

References 77 publications

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“…Compared to the DF pathway, the PF pathway results in a more specific and efficient bioethanol production [186]. The PF pathway comprises two steps: photosynthesis and fermentation.…”

Section: Alcoholic Fermentationmentioning

confidence: 99%

From Microalgae to Bioenergy: Recent Advances in Biochemical Conversion Processes

et al. 2023

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Concerns about rising energy demand, fossil fuel depletion, and global warming have increased interest in developing and utilizing alternate renewable energy sources. Among the available renewable resources, microalgae biomass, a third-generation feedstock, is promising for energy production due to its rich biochemical composition, metabolic elasticity, and ability to produce numerous bioenergy products, including biomethane, biohydrogen, and bioethanol. However, the true potential of microalgae biomass in the future bioenergy economy is yet to be realized. This review provides a comprehensive overview of various biochemical conversion processes (anaerobic digestion, direct biophotolysis, indirect biophotolysis, photo fermentation, dark fermentation, microalgae-catalyzed photo fermentation, microalgae-catalyzed dark fermentation, and traditional alcoholic fermentation by ethanologenic microorganisms) that could be adapted to transform microalgae biomass into different bioenergy products. Recent advances in biochemical conversion processes are compiled and critically analyzed, and their limitations in terms of process viability, efficacy, scalability, and economic and environmental sustainability are highlighted. Based on the current research stage and technological development, biomethane production from anaerobic digestion and bioethanol production from traditional fermentation are identified as promising methods for the future commercialization of microalgae-based bioenergy. However, significant challenges to these technologies’ commercialization remain, including the high microalgae production costs and low energy recovery efficiency. Future research should focus on reducing microalgae production costs, developing an integrated biorefinery approach, and effectively utilizing artificial intelligence tools for process optimization and scale-up to solve the current challenges and accelerate the development of microalgae-based bioenergy.

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“…Compared to the DF pathway, the PF pathway results in a more specific and efficient bioethanol production [186]. The PF pathway comprises two steps: photosynthesis and fermentation.…”

Section: Alcoholic Fermentationmentioning

confidence: 99%

From Microalgae to Bioenergy: Recent Advances in Biochemical Conversion Processes

et al. 2023

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“…Cyanobacteria derived ethanol do not produce any remnant, ash, smoke and greenhouse gases in any form therefore serves as an eco-friendly fuel (Luo et al, 2010 ). Multiple ethanologenic cassette insertions inside the Synechocystis genome have been shown to be a successful technique for increasing ethanol titers (Gundolf et al, 2019 ; Wang et al, 2020 ). However, in cyanobacteria, plasmid-based ethanol-generating systems have been highly effective and have frequently resulted in rather large ethanol titers (Dühring et al, 2017 ).…”

Section: Cyanobacteria: a Sustainable Biofactory For Biofuel And Biop...mentioning

confidence: 99%

Cyanobacteria as a Promising Alternative for Sustainable Environment: Synthesis of Biofuel and Biodegradable Plastics

et al. 2022

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With the aim to alleviate the increasing plastic burden and carbon footprint on Earth, the role of certain microbes that are capable of capturing and sequestering excess carbon dioxide (CO2) generated by various anthropogenic means was studied. Cyanobacteria, which are photosynthetic prokaryotes, are promising alternative for carbon sequestration as well as biofuel and bioplastic production because of their minimal growth requirements, higher efficiency of photosynthesis and growth rates, presence of considerable amounts of lipids in thylakoid membranes, and cosmopolitan nature. These microbes could prove beneficial to future generations in achieving sustainable environmental goals. Their role in the production of polyhydroxyalkanoates (PHAs) as a source of intracellular energy and carbon sink is being utilized for bioplastic production. PHAs have emerged as well-suited alternatives for conventional plastics and are a parallel competitor to petrochemical-based plastics. Although a lot of studies have been conducted where plants and crops are used as sources of energy and bioplastics, cyanobacteria have been reported to have a more efficient photosynthetic process strongly responsible for increased production with limited land input along with an acceptable cost. The biodiesel production from cyanobacteria is an unconventional choice for a sustainable future as it curtails toxic sulfur release and checks the addition of aromatic hydrocarbons having efficient oxygen content, with promising combustion potential, thus making them a better choice. Here, we aim at reporting the application of cyanobacteria for biofuel production and their competent biotechnological potential, along with achievements and constraints in its pathway toward commercial benefits. This review article also highlights the role of various cyanobacterial species that are a source of green and clean energy along with their high potential in the production of biodegradable plastics.

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“…This displayed the highest published ethanol productivity of 0.29 g/L/d [ 29 ]. This approach of multiple ethanologenic cassette insertions within the genome of Synechocystis has become a proven strategy to increase ethanol titres [ 24 , 28 , 29 , 37 , 41 ]. However, plasmid-based ethanol-producing systems are also quite successful in cyanobacteria, and have often led to relatively high ethanol titres [ 33 , 40 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria

Andrews

Faulkner

Toogood

et al. 2021

Biotechnol Biofuels

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Current industrial bioethanol production by yeast through fermentation generates carbon dioxide. Carbon neutral bioethanol production by cyanobacteria uses biological fixation (photosynthesis) of carbon dioxide or other waste inorganic carbon sources, whilst being sustainable and renewable. The first ethanologenic cyanobacterial process was developed over two decades ago using Synechococcus elongatus PCC 7942, by incorporating the recombinant pdc and adh genes from Zymomonas mobilis. Further engineering has increased bioethanol titres 24-fold, yet current levels are far below what is required for industrial application. At the heart of the problem is that the rate of carbon fixation cannot be drastically accelerated and carbon partitioning towards bioethanol production impacts on cell fitness. Key progress has been achieved by increasing the precursor pyruvate levels intracellularly, upregulating synthetic genes and knocking out pathways competing for pyruvate. Studies have shown that cyanobacteria accumulate high proportions of carbon reserves that are mobilised under specific environmental stresses or through pathway engineering to increase ethanol production. When used in conjunction with specific genetic knockouts, they supply significantly more carbon for ethanol production. This review will discuss the progress in generating ethanologenic cyanobacteria through chassis engineering, and exploring the impact of environmental stresses on increasing carbon flux towards ethanol production.

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Evaluation of New Genetic Toolkits and Their Role for Ethanol Production in Cyanobacteria

Cited by 7 publications

References 77 publications

From Microalgae to Bioenergy: Recent Advances in Biochemical Conversion Processes

From Microalgae to Bioenergy: Recent Advances in Biochemical Conversion Processes

Cyanobacteria as a Promising Alternative for Sustainable Environment: Synthesis of Biofuel and Biodegradable Plastics

Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria

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