Evaluation of osteoinductive and endothelial differentiation potential of Platelet‐Rich Plasma incorporated Gelatin‐Nanohydroxyapatite Fibrous Matrix

Anjana, Jain; Kuttappan, Shruthy; Keyan, Kripa S.; Nair, Manitha B.

doi:10.1002/jbm.b.33605

Cited by 13 publications

(3 citation statements)

References 39 publications

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“…In a former study, we reported a positive effect of PRP on endothelial cells due to the released growth factors from PRP . We and others demonstrated that PRP induces homing, adhesion, cell proliferation, migration and the osteoinductive potential of osteoblasts and mesenchymal stem cells as well as the proliferation and endothelial differentiation capacity of endothelial cells …”

Section: Discussionmentioning

confidence: 86%

Influence of different calcium phosphate ceramics on growth and differentiation of cells in osteoblast-endothelial co-cultures

Ritz

Götz

Baranowski

et al. 2016

J. Biomed. Mater. Res.

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Strategies for improvement of angiogenesis and vasculogenesis using different cells and materials are paramount aims in the field of bone tissue engineering. Thereby, the interaction between different cell types and scaffold materials is crucial for growth, differentiation, and long-term outcomes of tissue-engineered constructs. In this study, we evaluated the interaction of osteoblasts and endothelial cells in three-dimensional tissue-engineered constructs using beta tricalciumphosphate (β-TCP, [ß-Ca (PO ) ]) and calcium-deficient hydroxyapatite (CDHA, [Ca (PO ) (HPO )OH]) ceramics as scaffolds. We focused on initial cell organization, cell proliferation, and differential expression of osteoblastic and endothelial markers employing monocultures and co-cultures of endothelial cells of two different origins [human umbilical vein endothelial cells (HUVECs) and outgrowth endothelial cells (OECs)] with primary human osteoblasts (hOBs). Despite different chemical and physical characteristics of CDHA and β-TCP ceramics, similar patterns in cell growth, differentiation, and gene expression were detected in tissue-engineered constructs consisting of hOB, HUVEC, and HUVEC/hOB-co-cultures. Under dynamic cell culture conditions we found proliferation of these cells with stable endothelial and osteoblastic differentiation patterns. Both material types are highly biocompatible with these cells providing a promising perspective for the future research. In this study, both materials did not support growth and differentiation of OEC. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1950-1962, 2017.

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Section: Discussionmentioning

confidence: 86%

Influence of different calcium phosphate ceramics on growth and differentiation of cells in osteoblast-endothelial co-cultures

Ritz

Götz

Baranowski

et al. 2016

J. Biomed. Mater. Res.

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“…Furthermore, gelatin-calcium phosphate nanofibers, composed of β-TCP nanoparticles incorporated into electropunk gelatin nanofibers, improved proliferation and osteogenic differentiation of rat MSCs compared to pure calcium phosphate nanofibers (Kuttappan et al, 2016). Lastly, gelatin is also an ideal carrier for proteins and various growth factors (Krishnan et al, 2015;Anjana et al, 2016). For example, Gelatin/HA fibrous scaffold with platelet-rich plasma gel was able to induce the differentiation of MSCs into an osteogenic cell line (Anjana et al, 2016).…”

Section: Gelatin: a Collagen Degradation Product For Cell Adhesion Dmentioning

confidence: 99%

“…Lastly, gelatin is also an ideal carrier for proteins and various growth factors (Krishnan et al, 2015;Anjana et al, 2016). For example, Gelatin/HA fibrous scaffold with platelet-rich plasma gel was able to induce the differentiation of MSCs into an osteogenic cell line (Anjana et al, 2016).…”

Section: Gelatin: a Collagen Degradation Product For Cell Adhesion Dmentioning

confidence: 99%

Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine

Zhang

Zhao

et al. 2020

Front. Bioeng. Biotechnol.

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Bone is a dynamic organ with high regenerative potential and provides essential biological functions in the body, such as providing body mobility and protection of internal organs, regulating hematopoietic cell homeostasis, and serving as important mineral reservoir. Bone defects, which can be caused by trauma, cancer and bone disorders, pose formidable public health burdens. Even though autologous bone grafts, allografts, or xenografts have been used clinically, repairing large bone defects remains as a significant clinical challenge. Bone tissue engineering (BTE) emerged as a promising solution to overcome the limitations of autografts and allografts. Ideal bone tissue engineering is to induce bone regeneration through the synergistic integration of biomaterial scaffolds, bone progenitor cells, and bone-forming factors. Successful stem cell-based BTE requires a combination of abundant mesenchymal progenitors with osteogenic potential, suitable biofactors to drive osteogenic differentiation, and cell-friendly scaffold biomaterials. Thus, the crux of BTE lies within the use of cell-friendly biomaterials as scaffolds to overcome extensive bone defects. In this review, we focus on the biocompatibility and cell-friendly features of commonly used scaffold materials, including inorganic compound-based ceramics, natural polymers, synthetic polymers, decellularized extracellular matrix, and in many cases, composite scaffolds using the above existing biomaterials. It is conceivable that combinations of bioactive materials, progenitor cells, growth factors, functionalization techniques, and biomimetic scaffold designs, along with 3D bioprinting technology, will unleash a new era of complex BTE scaffolds tailored to patient-specific applications.

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Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

Zhao

Feng

2020

Adv Healthcare Materials

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Cardiovascular devices have been widely applied in the clinical treatment of cardiovascular diseases. However, poor hemocompatibility and slow endothelialization on their surface still exist. Numerous surface engineering strategies have mainly sought to modify the device surface through physical, chemical, and biological approaches to improve surface hemocompatibility and endothelialization. The alteration of physical characteristics and pattern topographies brings some hopeful outcomes and plays a notable role in this respect. The chemical and biological approaches can provide potential signs of success in the endothelialization of vascular device surfaces. They usually involve therapeutic drugs, specific peptides, adhesive proteins, antibodies, growth factors and nitric oxide (NO) donors. The gene engineering can enhance the proliferation, growth, and migration of vascular cells, thus boosting the endothelialization. In this review, the surface engineering strategies are highlighted and summarized to improve hemocompatibility and rapid endothelialization on the cardiovascular devices. The potential outlook is also briefly discussed to help guide endothelialization strategies and inspire further innovations. It is hoped that this review can assist with the surface engineering of cardiovascular devices and promote future advancements in this emerging research field.

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Evaluation of osteoinductive and endothelial differentiation potential of Platelet‐Rich Plasma incorporated Gelatin‐Nanohydroxyapatite Fibrous Matrix

Cited by 13 publications

References 39 publications

Influence of different calcium phosphate ceramics on growth and differentiation of cells in osteoblast-endothelial co-cultures

Influence of different calcium phosphate ceramics on growth and differentiation of cells in osteoblast-endothelial co-cultures

Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine

Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

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