Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron

Matand, Kanyand; Prakash, Channapatna S.

doi:10.1016/j.jbiotec.2007.02.014

Cited by 37 publications

(23 citation statements)

References 27 publications

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“…Recently, the morpho-regulatory potential of TDZ has led to its application in plant tissue culture for the development of feasible morphogenetic systems ( Figure 1). TDZ emerged as an effective bioregulant in cell and tissue cultures in wide array of plant species (Li et al, 2000;Hosseini-Nasr and Rashid, 2000;Svetla et al, 2003;Matand and Prakash, 2007).…”

Section: Introductionmentioning

confidence: 99%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

200

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Thidiazuron (TDZ) has gained a considerable attention during past decades due to its efficient role in plant cell and tissue culture. Wide array of physiological responses were observed in response to TDZapplication in different plant species. TDZ has shown both auxin and cytokinin like effects, although, chemically, it is totally different from commonly used auxins and cytokinins. A number of biological (physiological and biochemical) events in cells are induced or enhanced by TDZ, but the mode of action of TDZ is yet unknown. However, varieties of underlying mechanisms were revealed by reports showing how morphogenic events were induced by application of TDZ. Other reports showed that TDZ may modify endogenous plant growth regulators, either directly or indirectly and produce reactions in cell/tissue, necessary for its division/regeneration. Other possibilities include modification in cell membrane, energy levels, nutrient absorption, transport and assimilation, etc. In this review, recent advancements in TDZ application in plant sciences are discussed.

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Section: Introductionmentioning

confidence: 99%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

200

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“…For legumes, a major limitation of the available protocols for in vitro regeneration is that they are highly genotype-specific and only a few of the genotypes are amenable to regeneration after Agrobacterium-mediated transformation (Matand and Prakash 2007;Bhattacharjee et al 2010). This study involved four phenotypes of peanuts, which were bred independently by us: one (Huayu 17) that matures early and has relatively large fruit, two (Huayu 20 and Huayu 26) that mature early and have relatively small fruit, and one (Luhua 6 hao) that matures extremely early and has relatively small fruit.…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants

ChenMingna¹,

YangQingli²,

WangTong³

et al. 2015

Can. J. Plant Sci.

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S. 2015. Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants. Can. J. Plant Sci. 95: 735Á744. Four genotypes of peanut and two sources of explants (cotyledon and mesocotyl) were tested for their susceptibility to genetic transformation by the Agrobacterium tumefaciens strain LBA4404 that harbored the binary vector pCAMBIA1301. This plasmid contains the hygromycin phosphotransferase (hpt) and b-glucuronidase (GUS) genes, each under the control of a CaMV35S promoter. Comparative analyses of regeneration and transformation efficiencies indicated that mesocotyl was a better target tissue than cotyledon, and peanut genotypes of that mature early and have relatively small seeds (such as Huayu 26 and Huayu 20) were shown to be comparatively responsive to transformation. Sonication of explants soaked with solutions containing Agrobacterium was shown to optimize transformation. Culture of explants on medium supplemented with 3 mg L(1 indole-3-butyric acid and 0.1 mg L (1 napthaleneacetic acid enabled vigorous rooting from almost all transgenic shoots. More than 85% of the transplanted plants could produce morphologically normal flowers and pods with viable seeds. Phenotypic and genotypic monitoring of the inheritance of hpt and GUS genes through two generations indicated the expected 3:1 inheritance. Our results make Agrobacterium-mediated transformation a viable and useful tool for both breeding and functional genomic analysis of peanut.

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“…Hence, the cultivars like TAG 24 and Western-55 can be applied as a model system for gene transfer techniques. TDZ is a potent cytokinin that promotes shoot formation and plant regeneration via organogenesis and somatic embryogenesis in cultivated peanut within a short duration while in culture [2,11,12]. Visser et al (1992) first described the effective substitution by TDZ of both auxin and cytokinin requirement of the geranium tissue culture system [13].…”

Section: F-value Ns Ns Nsmentioning

confidence: 99%

“…Control explants showed callus significantly. Another report by Matand and Prakash(2007) was that the use of TDZ (0.5 mg/l) for 10 days appeared to be sufficient to promote shoot formation in explants across peanut botanical groups [11].…”

Section: F-value Ns Ns Nsmentioning

confidence: 99%

“…In vitro regeneration, a key requisite in the development of transgenic peanut plants is highly genotype dependent. Differences in the in vitro response among the non Indian genotypes belong to Spanish, Runner, Virginia, and Valencia types had been extensively studied and reported [2]. Though in India, more than a hundred varieties of peanut are released for cultivation based on its agronomic importance (SB11, JL 24, WesternHB-55, TAG 24, ICGS 1, GG 20, K134 etc), literature pertaining to in vitro studies is very much limited to SB11 and JL 24.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Indian Peanut on the Induction of Caulogenic Buds in Vitro

Raman¹,

Dagne²,

Malpathak³

et al. 2012

IJAF

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The present work has been undertaken to study the in vitro response in widely used cultivars WesternHB-55, TAG 24 and SB11 of peanut having desired agronomic characteristics. The mature embryo derived leaflet explants of all three peanut genotypes were found morphogenic in vitro. When comparing the effects of TDZ and BA with respect to caulogenic response, it can be inferred that TDZ is a more potent inducer of caulogenesis in vitro than BA. All genotypes responded equally to different concentrations of BA. WesternHB-55 responded better than others which results in high proliferation rate in vitro. The present study emphasizes on the usage of WesternHB-55 germplasm through adventitious organogenesis pathway in transformation studies (biolistic gun approach), for the production of proteins, vaccines enzymes etc.

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Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron

Cited by 37 publications

References 27 publications

Thidiazuron: A multi-dimensional plant growth regulator

Thidiazuron: A multi-dimensional plant growth regulator

Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants

Evaluation of Indian Peanut on the Induction of Caulogenic Buds <i>i</i><i>n </i><i>Vitro</i>

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