Evaluation of Phage Delivery Systems on Induced Motile<i>Aeromonas</i>Septicemia in<i>Oreochromis niloticus</i>

Gordola, Kathleen May C.; Boctuanon, Fatima Alesandra U.; Diolata, Raylene Audrey A.; Pedro, Maria Bernadette D.; Gutierrez, Tracey Antaeus D.; Papa, Rey Donne S.; Papa, Donna May D.

doi:10.1089/phage.2020.0020

Cited by 5 publications

(1 citation statement)

References 33 publications

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“…One is the lack of adequately documented clinical research. Although there is already a history of phage therapy studies (El-Shibiny and El-Sahhar 2017) -including administration, delivery, and pharmacokinetics of phages (Ahmadi et al 2016;Gordola et al 2020;Dabrowska 2019;Qadir et al 2018) -there are no established protocols for the fixed amount of dose and frequency and the recommended course of administration and duration of the treatment, especially to the therapy of Salmonella. The knowledge concerning phage behavior in vivo is minimal.…”

Section: Introductionmentioning

confidence: 99%

Comparing the Efficacy of Bacteriophages and Antibiotics in Treating Salmonella enterica Serovar Typhimurium on Streptomycin-pretreated Mice

Acebes¹,

Cuento²,

Serna³

et al. 2021

Philipp J Sci

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The misuse of antibiotics had contributed significantly to the development of antibiotic resistance, hence decreasing its efficacy. Non-typhoidal Salmonella is a global problem causing gastroenteritis in humans. Since there are various antibiotics in treating Salmonella infections, its potential to become multidrug-resistant remains a considerable problem. The use of host-specific bacteriophages as a biocontrol is one possible intervention by which Salmonella colonization could be reduced. Isolated and purified from sewage water, a dose of 4.30 x 1010 PFU (plaque-forming units) Salmonella phages were used to treat gastroenteritis on streptomycin-mouse models. Lack of treatment yielded 2.06 x 106 CFU (colony-forming units)/g of Salmonella 24 h post-infection, which exponentially dropped to 3.80 x 103 CFU/g 24 h post-infection in bacteriophage treatment before gradually decreasing. However, the antibiotic treatment inhibited growth with 3.58 x 102 CFU/g 24 h post-infection, but it did not retain the decrease of the pathogen. Results also showed that the microflora of the gut was preserved with the appearance of various colonies in phage treatment, which aided recovery in a phenomenon known as “microbial interference.” In contrast, antibiotic treatment depleted the normal microflora, allowing colonization of Salmonella and resurgence of pathogenic growth in the gut. Bacteriophage therapy demonstrated a significant decrease in bacterial load when compared to the antibiotic ciprofloxacin. The high specificity of the treatment also allowed the phages to synergize with the gut microbiota, thereby inhibiting colonization and further increase in bacterial growth.

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Section: Introductionmentioning

confidence: 99%

Comparing the Efficacy of Bacteriophages and Antibiotics in Treating Salmonella enterica Serovar Typhimurium on Streptomycin-pretreated Mice

Acebes¹,

Cuento²,

Serna³

et al. 2021

Philipp J Sci

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Bacteriophage therapy: an emerging paradigm in fish disease management

Nokhwal

Anand

Ravikant³

et al. 2022

Aquacult Int

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Multilocus sequence typing, antimicrobial susceptibility, and phage discovery of motile Aeromonas species from the pond-cultured goldfish Carassius auratus on two farms in Japan

Fudeshima,

Kitaoka,

Kaneko

et al. 2024

Fish Sci

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Motile Aeromonas spp., including Aeromonas hydrophila, A. caviae, and A. veronii biovar sobria can cause motile aeromonad disease and pose serious threats to the aquaculture industry. Motile aeromonads showing high minimum inhibitory concentrations (MICs) to widely used various antibiotics in global aquaculture production have been reported. Several studies have shown that A. hydrophila phages are effective in treating infected fish. However, in Japan, epidemiological reports and phage studies of motile Aeromonas spp. isolated from fish are scarce. In this work, we visited two goldfish farms in Japan between 2020 and 2021, and obtained 33 motile Aeromonas isolates from Carassius auratus exhibiting symptoms of motile aeromonad disease. Multilocus sequence typing analysis revealed that the isolated motile aeromonads belonged to a wide variety of sequence types (STs) including new STs. The ST2007 accounted for 50% (6/12) of A. hydrophila isolates, and each was isolated at different timepoints and farms. The isolated bacteria exhibited high MICs for oxolinic acid, nitrofural, sulfamerazine, and oxytetracycline; 13 isolates (39%) which presented with MICs above commercially used concentrations of oxolinic acid, nitrofural, and sulfamerazine in Japan and were classified as non-wild types for oxytetracycline. The subsequently isolated phages showed high specificity for the motile aeromonads isolates.

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Evaluation of Phage Delivery Systems on Induced MotileAeromonasSepticemia inOreochromis niloticus

Cited by 5 publications

References 33 publications

Comparing the Efficacy of Bacteriophages and Antibiotics in Treating Salmonella enterica Serovar Typhimurium on Streptomycin-pretreated Mice

Comparing the Efficacy of Bacteriophages and Antibiotics in Treating Salmonella enterica Serovar Typhimurium on Streptomycin-pretreated Mice

Bacteriophage therapy: an emerging paradigm in fish disease management

Multilocus sequence typing, antimicrobial susceptibility, and phage discovery of motile Aeromonas species from the pond-cultured goldfish Carassius auratus on two farms in Japan

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