Evaluation of physiochemical factors affecting high-frequency plant regeneration of Blyttia spiralis (synonym: Pentatropis spiralis), a threatened climber of medicinal value

Patel, Ashok Kumar; Lodha, Deepika; Ram, Kheta; Shekhawat, S. S.; Shekhawat, N. S.

doi:10.1007/s11627-015-9738-1

Cited by 17 publications

(10 citation statements)

References 36 publications

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“…Similarly, NOA was also found less efficient for ex vitro rooting in comparison to IBA. This study reveals the advantageous role of IBA in rooting and is in accordance with many preceding reports on different plants such as Salvadora oleoides (Phulwaria et al 2014), Cadaba fruticosa (Lodha et al 2015), Blyttia spiralis (Patel et al 2016), etc. This is the first successful report of ex vitro rooting in M. dioica and probably in family Cucurbitaceae with a significant response rate (90%).…”

Section: Ex Vitro Rooting and Acclimatizationsupporting

confidence: 92%

“…Although shoots were formed after the second and third passages, however their number decreased with each passage. This method of shoot multiplication has also been used in Cadaba fruticosa (Lodha et al 2015) and Blyttia spiralis (Patel et al 2016). In the second approach, in vitro raised shoots were multiplied through subculturing.…”

Section: Shoot Bud Induction Multiplication and Maintenance Of Culturesmentioning

confidence: 99%

“…In this study, we observed that incorporation of an auxin (IAA) at a lower concentration (0.1 mg L -1 ) significantly increased the number of shoots by promoting axillary branching. There are several researches indicating that an appropriate auxin(s)-cytokinin(s) combination is mandatory for a better shoot proliferation/growth (Phulwaria et al 2014;Masondo et al 2015;Patel et al 2016). Su and Zhang (2014) explained that plant regeneration is not individually regulated by either auxins or cytokinins alone but it is the product of a complex mechanism of interconnecting hormonal signaling pathways.…”

Section: Shoot Bud Induction Multiplication and Maintenance Of Culturesmentioning

confidence: 99%

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An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Choudhary

Patel

Harish

et al. 2017

Physiol Mol Biol Plants

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Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L BAP, 0.1 mg L IAA and additives (50 mg L ascorbic acid, 25 mg L each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female , a popular summer vegetable crop.

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Section: Ex Vitro Rooting and Acclimatizationsupporting

confidence: 92%

Section: Shoot Bud Induction Multiplication and Maintenance Of Culturesmentioning

confidence: 99%

Section: Shoot Bud Induction Multiplication and Maintenance Of Culturesmentioning

confidence: 99%

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An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Choudhary

Patel

Harish

et al. 2017

Physiol Mol Biol Plants

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“…Indole-3aceticacidwasreportedtobelesseffectiveforthe ex vitro rooting of the shoots of M. mercurialisand83%of shootswererootedwith3.0rootspershootwhenpulsed with 100 mg l -1 IAA (Table 5). The superiority of IBA in ex vitro rooting has also been reported in Hemidesmus indicus (Shekhawat & Manokari 2016b) and Blyttia spiralis (Patel et al 2016) because the absorption of IBA was reported to befasterthanotherauxins (VanDerKriekenetal.1993).…”

Section: Ex Vitro Rooting Of Shoots and Field Transfer Of Plantletsmentioning

confidence: 69%

Optimization of in vitro and ex vitro regeneration and micromorphological studies of Micrococca mercurialis (L.) Benth.

Manokari¹,

Shekhawat²

2017

Bot Pac

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A regeneration protocol has been successfully developed using nodal segments and leaf explants of Micrococca mercurialis (L.) Benth. The surface sterilized leaf explants were induced to proliferative green colored callus on Murashige and Skoog (MS) me dium supplemented with 0.5 mg l -1 of 2,4-dichlorophenoxyacetic acid (2,4-D). Shoots were regenerated from the callus on MS medium sup plemen ted with 0.5 mg l -1 6-benzylaminopurine (BAP). The nodal shoot segments res ponded on MS medium augmented with 1.0 mg l -1 BAP. The shoots were mul ti pliedonMSmediumfortifiedwith0.5mgl -1 each of BAP, kinetin (Kin) and 0.1 mg l -1 α-naphthaleneaceticacid(NAA).Themaximum17.3shootswith 8.58 cm average length were resulted on this media combination. The shoots were rooted ex vitro when pulse treated with 100 mg l -1 indole-3 butyric acid (IBA).Theplant letsweresuccessfullyestablishedinthefieldwith96%success rate. Mic ro mo rphological studies were conducted for the successive stages of in vitro, ex vitro and in vivo grown plants to analyze the foliar developmental changes taken place across the environments. It has been concluded that the abnormal mor phophysiology of in vitro plantlets were repaired while acclimatization under thegreenhouseconditionsbeforetransferringtothefield,whichspeedupthe micropropagation process and increase the survival rate of the plantlets.

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“…Salt strength of MS medium is an important factor in influencing the rooting efficiency in several plant species (Kher et al, 2015;Shekhawats and Manokari, 2016;Patel et al, 2016). Half strength MS medium was reported superior (100% response) as compared to the full strength (74% response) and 1/4 th strength (81% response) MS media for rhizogenesis in T. ulmifolia shoots (Tab.…”

Section: In Vitro Rooting and Hardening Of Plantletsmentioning

confidence: 99%

Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

Manokari

Shekhawat²

2018

Folia Horticulturae

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The present study reports an efficient in vitro propagation system for Turnera ulmifolia using nodal segments as explants. Turnera ulmifolia (Passifloraceae) is an important garden plant with multipotent medicinal values. Effective shoot proliferation was achieved on agar gelled MS medium (Murashige and Skoog, 1962). The maximum number of shoots (8.3 ± 0.57) per initial explant was obtained on MS medium supplemented with 8.88 mM of 6-benzylaminopurine (BAP) and 0.54 mM of α-naphthalene acetic acid (NAA). The highest number of shoots (59.5 ± 2.10) proliferated on semi-solid MS medium (with agar) augmented with 2.22 mM of BAP and 2.32 mM of kinetin (Kin) along with 0.54 mM of NAA. Longer (4-5 cm) and healthy shoots were rooted (12.0 ± 0.10 roots per shoot) on half-strength MS medium fortified with 9.84 mM of indole-3 butyric acid (IBA). The in vitro regenerated plantlets were hardened in the greenhouse and transferred to the field. Significant developmental changes were observed in the foliar micromorphology of in vitro raised plantlets when these were transferred to the field. The stomatal index was gradually reduced (26.72 to 21.25) in the leaves from in vitro to field environments. But, vein-islets and veinlet terminations (13.4 and 7.6) were increased (39.7 and 18.4) respectively from in vitro to in vivo grown plants. Simple, unicellular, less frequent and underdeveloped trichomes were observed with the leaves of in vitro plants but fully developed trichomes recorded in the field transferred plants. The study could help in understanding the response and adaptation of tissue culture raised plantlets towards changed environmental conditions.

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Evaluation of physiochemical factors affecting high-frequency plant regeneration of Blyttia spiralis (synonym: Pentatropis spiralis), a threatened climber of medicinal value

Cited by 17 publications

References 36 publications

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Optimization of in vitro and ex vitro regeneration and micromorphological studies of Micrococca mercurialis (L.) Benth.

Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

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