Evaluation of plasma circ_0006282 as a novel diagnostic biomarker in colorectal cancer

Abstract

Background Nowadays, non‐invasive and rapid detection of cancers through molecular biomarkers has received much attention. Therefore, this study investigated the non‐invasive and rapid diagnosis of colorectal cancer through one of the newest biomarkers (circular RNA). Methods For this purpose, we collected tumoral, adjacent normal tissue, and plasma samples from 100 colorectal cancer (CRC) patients, 25 postoperative CRC patients, 28 colitis patients, and 108 healthy donors. First Illumina high‐throughput (Hi Seq 2000) sequencing was performed to identify known and novel differentially expressed circRNAs in the cancerous and adjacent normal tissues (n = 3). We used quantitative real‐time fluorescent polymerase chain reaction (qRT‐PCR) to detect the expression level of hsa_circ_0006282 among the different samples. Moreover, inter‐ and intra‐assays were performed to evaluate the potential of hsa_circ_0006282 as being a biomarker. The receiver operating characteristic curve (ROC) was drawn to appraise its diagnostic efficacy, and the sensitivity of this circ RNA was evaluated. Results Based on RNA‐sequencing results circ_0006282, cirs7, circ‐0001313, circ_0055625, circ_000984, circ_0055625, circ_0001178, circ_0071589, circ‐001569 were upregulated, and circ‐ITGA7, circ‐CDYL, circITCH, circ_0026344, circ_0000038, circ_0002220, circ_0067480, circIGHV3‐20‐1, circ_104916, circ_0009361 were downregulated circRNA. The hsa_circ_0006282 was the highest upregulated differentially expressed circRNA. Expression evaluation of this circRNA on different samples showed upregulation in CRC tissues (p < 0.0001) and plasma samples of CRC patients in comparison to healthy controls (p < 0.0001), while the area under the curve (AUC) was 0.831 (95% CI: 0.779–0.883). Expression of hsa_circ_0006282 in CRC patients decreased to normal after surgery (p < 0.0001). Our results showed high specificity and sensitivity of CRC detection when hsa_circ_0006282, carcinoembryonic antigen (CEA), and carbohydrate antigen199 (CA199) are combined. Conclusion Plasma hsa_circ_0006282 can be used as a novel diagnostic and dynamic monitoring biomarker in CRC patients.

Identification and diagnostic potential of hsa_circ_101303 in colorectal cancer: unraveling a regulatory network

“…Moreover, our findings revealed that plasma levels of hsa_circ_101303 offer moderate diagnostic accuracy for CRC (AUC: 0.738) as determined through RT-qPCR assay. While this aligns with the observations by Radanova et al [ 23 ], our results suggest a slightly lower diagnostic accuracy compared to the studies conducted by Ye et al [ 22 ] and Zhu et al reports [ 24 ]. These collective results highlight the potential of hsa_circ_101303 as a promising diagnostic biomarker for CRC.…”

“…Radanova et al [ 23 ] reported moderated diagnostic values for plasma levels of hsa_circ_0001445, hsa_circ_0003028, hsa_circ_0007915, and hsa_circ_0008717 in CRC, with AUC values of 0.739, 0.693, 0.776, and 0.626, respectively. Furthermore, another study demonstrated that plasma levels of hsa_circ_0006282 hold significant diagnostic potential for CRC, with an AUC of 0.831 [ 24 ]. In our investigation, we observed an upregulation of hsa_circ_101303 in both plasma and tissue samples of CRC, indicating its consistent expression in these compartments.…”

Biological functions, mechanisms, and clinical significance of circular RNA in colorectal cancer

“…Furthermore, the study revealed that plasma circ_0006282 can differentiate between patients with colorectal cancer and colitis. Taken together, these findings suggest that plasma circ_0006282 can serve as a promising diagnostic and dynamic monitoring biomarker for CRC ( 160 ).…”

HSPB6 Is Depleted in Colon Cancer Patients and Its Expression Is Induced by 5-aza-2′-Deoxycytidine In Vitro

“…Our geneMANIA result indicates the strongest genetic interaction with HSPB6 is ITGA7 [ 52 , 53 ], which has been upregulated in cells derived from embryonic stem cells [ 54 ]. Interestingly, HSPB6 [ 21 ] and ITGA7 [ 55 , 56 ] were downregulated in colorectal cancer, suggesting potential transcriptional positive regulation. In contrast, HSPB1 has the strongest physical interaction with HSPB6 [ 57 , 58 ].…”