Evaluation of plasma microsampling for dried plasma spots (DPS) in quantitative LC-MS/MS bioanalysis using ritonavir as a model compound

Li, Wenkui; Doherty, John; Favara, Sarah; Breen, Christopher; Flarakos, Jimmy; Tse, Francis L. S.

doi:10.1016/j.jchromb.2015.03.026

Cited by 21 publications

(18 citation statements)

References 6 publications

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“…A major advantage of plasma microsampling is sample homogeneity and absence of blood cells. Therefore, the HCT effect can be ignored in analysis (Parker et al 2015a; Li et al 2015a). In some cases, the concentration of molecules in plasma alone provides sufficient information for disease diagnosis.…”

Section: Blood Samplingmentioning

confidence: 99%

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A review of microsampling techniques and their social impact

Lei

Prow

2019

Biomed Microdevices

108

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Conventional skin and blood sampling techniques for disease diagnosis, though effective, are often highly invasive and some even suffer from variations in analysis. With the improvements in molecular detection, the amount of starting sample quantity needed has significantly reduced in some diagnostic procedures, and this has led to an increased interest in microsampling techniques for disease biomarker detection. The miniaturization of sampling platforms driven by microsampling has the potential to shift disease diagnosis and monitoring closer to the point of care. The faster turnaround time for actionable results has improved patient care. The variations in sample quantification and analysis remain a challenge in the microsampling field. The future of microsampling looks promising. Emerging techniques are being clinically tested and monitored by regulatory bodies. This process is leading to safer and more reliable diagnostic platforms. This review discusses the advantages and disadvantages of current skin and blood microsampling techniques.

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Section: Blood Samplingmentioning

confidence: 99%

“…The artificial interstitial fluid was located in a reservoir under a piece of excised mouse skin and was sampled by the microneedle array after penetrating the skin. Pictures retrieved from Li et al 2015a, b and Mohan et al 2017…”

Section: Blood Samplingmentioning

confidence: 99%

A review of microsampling techniques and their social impact

Lei

Prow

2019

Biomed Microdevices

108

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“…However, DPS/DSS samples, which are more homogenous in comparison with DBS, do not contain blood cells and thus do not show the haematocrit dependence. These samples are often used in different fi elds of clinical and pre-clinical analyses (94)(95)(96)(97)(98)(99).…”

Section: Similar Types Of Samplesmentioning

confidence: 99%

The dried blood spot sampling method in the laboratory medicine

Andrlová¹,

Kanďár²

2019

BLL

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The minimization is currently expanding in all fi elds of human life. The smaller size of analytical instruments and their higher sensitivity enables an analysis of small amounts of a biological material with a very low consumption of chemicals, what is economically and also environmentally benefi cial. A patient friendly and minimal invasive sample collection is therefore more than required. The dried blood spot (DBS) sampling standardly used in the newborn screening (NS) may be an option. The sample collection is simple, non-invasive, does not require trained medical personnel assistance and also storing and transportation of these samples is much easier in comparison with the whole blood samples. The DBS sampling method is used in the therapeutic drug monitoring or in the diagnosis of infectious diseases and its usage is still spreading. In some cases, it is still only a research object, but it shows a big potential for a future use. It could completely replace the whole venous blood collection in some cases, for example in the metabolic screening of diabetic patients or monitoring of treatment response, and it can overall simplify the sample collection and the transportation process (Tab. 1, Ref. 152).

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“…Plasma microsampling is currently on the horizon for quantitative bioanalysis in support of drug development (Bowen et al, 2013;Li et al, 2015). Briefly, a small volume of blood is collected into a microsampling capillary or plastic device which is then centrifuged for plasma.…”

mentioning

confidence: 99%

“…48 μl) of the resultant diluted plasma sample being subjected to extraction for LC-MS/MS analysis . Alternatively, the small volume of plasma can be spotted onto cellulose cards to obtain dried plasma spots (DPS) for multiple analyses (Li et al, 2015). DPS retains the many advantages of DBS but without the issues that hinder DBS application, as mentioned above.…”

mentioning

confidence: 99%

Application of tail vein serial microsampling for plasma or dried plasma spots in toxicokinetic assessment in rats using acetaminophen as the model compound

Dugyala

Devine

et al. 2020

Biomedical Chromatography

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In the current study, two groups of rats (five per group) were administered a single oral dose of 500 mg/kg acetaminophen. For toxicokinetic assessment, the Group 1 animals were bled via conventional sparse (two animals/time point) sublingual vein bleeding (~0.5 ml) with anesthesia, while the Group 2 animals were bled via serial tail vein microsampling (~0.075 ml) without anesthesia. All collected blood was processed for plasma. Each Group 2 plasma sample (~30 μl) was divided into ‘wet’ and ‘dried’ (dried plasma spots). All plasma samples were analyzed by LC–MS/MS for acetaminophen and its major metabolites acetaminophen glucuronide and acetaminophen sulfate. In addition, plasma and urine samples were collected for analysis of corticosterone and creatinine to assess stress levels. Comparable plasma exposure to acetaminophen and its two metabolites was observed in the plasma obtained via conventional sparse sublingual vein bleeding and serial tail vein microsampling and between the ‘wet’ and ‘dried’ plasma obtained by the latter. Furthermore, comparable corticosterone levels or corticosterone/creatinine ratios between the two groups suggested that serial microsampling without anesthesia did not increase the levels of stress as compared with conventional sampling with anesthesia, confirming the utility of microsampling for plasma or dried plasma spots in rodent toxicokinetic assessment.

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Evaluation of plasma microsampling for dried plasma spots (DPS) in quantitative LC-MS/MS bioanalysis using ritonavir as a model compound

Cited by 21 publications

References 6 publications

A review of microsampling techniques and their social impact

A review of microsampling techniques and their social impact

The dried blood spot sampling method in the laboratory medicine

Application of tail vein serial microsampling for plasma or dried plasma spots in toxicokinetic assessment in rats using acetaminophen as the model compound

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